modB Family assigned · medium auto-curated
H37Rv Rv1858 · MTBC0 mtbc0_001971 ·
264 aa · 2123811–2124605 (+) ·
RefSeq NP_216374.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | molybdenum ABC transporter permease ModB |
|---|---|
| MTBC0 PGAP re-annotation | ABC transporter permease |
| Revised (this work) | ABC transporter permease. Pfam: BPD_transp_1 (PF00528.28). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WG13
SwissProt · reviewed
· Inferred from homology
|
|---|---|
| UniProt name | Molybdenum transport system permease protein ModB |
| Curated function | Part of the binding-protein-dependent transport system ModABCD for molybdenum; probably responsible for the translocation of the substrate across the membrane. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
P Inorganic ion transport and metabolism
|
|---|---|
| Preferred name | modB |
| eggNOG description | ABC transporter |
| Orthologous group | COG4149 |
| EC number |
EC 3.6.3.29
|
| KEGG orthology |
K02017, K02018
|
| KEGG pathways |
map02010
|
| KEGG modules |
M00189
|
| Gene Ontology (6) |
GO:0005575, GO:0005623, GO:0005886, GO:0016020, GO:0044464, GO:0071944
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
BPD_transp_1 | PF00528.28 | 4.3e-15 | 69–250 | Binding-protein-dependent transport system inner membrane component |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: modC (molybdenum ABC transporter ATP-binding protein ModC), high confidence from genomic context alone (score 1000 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1859 modC |
molybdenum ABC transporter ATP-binding protein ModC | 999 | 1000 ctx | neighborhood:816 fusion:891 cooccurence:599 coexpression:662 database:900 |
Rv1857 modA |
molybdate ABC transporter substrate-binding lipoprotein ModA | 999 | 999 ctx | neighborhood:816 cooccurence:772 coexpression:886 database:800 textmining:902 |
Rv2397c cysA1 |
sulfate ABC transporter ATP-binding protein CysA | 937 | 929 ctx | fusion:503 cooccurence:563 coexpression:658 |
Rv1860 apa hyp |
hypothetical protein | 628 | 620 ctx | neighborhood:620 |
Rv2038c ugpC |
sugar ABC transporter ATP-binding protein | 563 | 563 ctx | fusion:474 |
Rv1856c |
oxidoreductase | 559 | 559 ctx | neighborhood:532 |
Rv2400c subI |
sulfate ABC transporter substrate-binding lipoprotein SubI | 553 | 536 ctx | cooccurence:516 |
Rv0869c moaA2 |
molybdenum cofactor biosynthesis protein MoaA | 522 | 464 | |
Rv1855c |
oxidoreductase | 459 | 459 ctx | neighborhood:457 |
Rv2920c amt |
ammonium transporter integral membrane protein | 436 | 436 | coexpression:426 |
Rv3859c gltB |
glutamate synthase large subunit | 433 | 433 | coexpression:400 |
Rv0865 mog |
molybdopterin biosynthesis protein | 447 | 427 | |
Rv0438c moeA2 |
molybdopterin molybdenumtransferase | 479 | 410 | |
Rv2832c ugpC |
sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC | 408 | 408 | |
Rv0866 moaE2 |
molybdopterin synthase catalytic subunit 2 | 439 | 384 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: molybdenum ABC transporter permease ModB
- MTBC0 PGAP product: ABC transporter permease
- Pfam (hmmscan --cut_ga): BPD_transp_1 PF00528.28 (E=4e-15)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216374.1)
- Domains: Pfam-A via hmmscan --cut_ga — BPD_transp_1 (PF00528.28)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG4149 - Curated reference: UniProt P9WG13 (SwissProt, reviewed; Inferred from homology)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
25 functional partner(s); context anchor
modC - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001971|Rv1858|modB MHPPTDLPRWVYLPAIAGIVFVAMPLVAIAIRVDWPRFWALITTPSSQTALLLSVKTAAASTVLCVLLGVPMALVLARSRGRLVRSLRPLILLPLVLPPVVGGIALLYAFGRLGLIGRYLEAAGISIAFSTAAVVLAQTFVSLPYLVISLEGAARTAGADYEVVAATLGARPGTVWWRVTLPLLLPGVVSGSVLAFARSLGEFGATLTFAGSRQGVTRTLPLEIYLQRVTDPDAAVALSLLLVVVAALVVLGVGARTPIGTDTR