ureG Resolved · high auto-curated
H37Rv Rv1852 · MTBC0 mtbc0_001965 ·
224 aa · 2118378–2119052 (+) ·
RefSeq NP_216368.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | urease accessory protein UreG |
|---|---|
| MTBC0 PGAP re-annotation | urease accessory protein UreG |
| Revised (this work) | Urease accessory protein UreG. Pfam: cobW (PF02492.26). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WFE3
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Urease accessory protein UreG |
| Curated function | Facilitates the functional incorporation of the urease nickel metallocenter. This process requires GTP hydrolysis, probably effectuated by UreG. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
K TranscriptionO Post-translational modification, protein turnover, chaperones
|
|---|---|
| Preferred name | ureG |
| eggNOG description | Facilitates the functional incorporation of the urease nickel metallocenter. This process requires GTP hydrolysis, probably effectuated by UreG |
| Orthologous group | COG0378 |
| KEGG orthology |
K03189
|
| Gene Ontology (8) |
GO:0003674, GO:0003824, GO:0003924, GO:0016462, GO:0016787, GO:0016817, GO:0016818, GO:0017111
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.366 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 1 synonymous, 1 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
cobW | PF02492.26 | 7.4e-37 | 28–197 | CobW/HypB/UreG, nucleotide-binding domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: ureF (urease accessory protein UreF), high confidence from genomic context alone (score 997 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1851 ureF |
urease accessory protein UreF | 999 | 997 ctx | neighborhood:874 coexpression:871 experimental:810 textmining:625 |
Rv0087 hycE |
formate hydrogenase HycE | 995 | 992 | experimental:989 textmining:439 |
Rv1850 ureC |
urease subunit alpha | 997 | 991 ctx | neighborhood:874 cooccurence:774 coexpression:722 textmining:692 |
Rv1848 ureA |
urease subunit gamma | 993 | 991 ctx | neighborhood:874 cooccurence:774 coexpression:716 |
Rv1849 ureB |
urease subunit beta | 996 | 989 ctx | neighborhood:874 cooccurence:774 coexpression:656 textmining:704 |
Rv1853 ureD |
urease accessory protein UreD | 992 | 983 ctx | neighborhood:882 coexpression:728 experimental:446 textmining:590 |
Rv1847 |
esterase | 712 | 713 ctx | neighborhood:712 |
Rv0073 |
glutamine ABC transporter ATP-binding protein | 450 | 393 | |
Rv2564 glnQ |
glutamine ABC transporter ATP-binding protein | 449 | 391 | |
Rv1843c guaB1 |
inosine-5'-monophosphate dehydrogenase | 474 | 313 | |
Rv3419c gcp |
O-sialoglycoprotein endopeptidase | 931 | 78 | textmining:929 |
Rv1862 adhA |
alcohol dehydrogenase A | 414 | 55 | textmining:406 |
Rv2362c recO |
DNA repair protein RecO | 410 | 49 | textmining:406 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: urease accessory protein UreG
- MTBC0 PGAP product: urease accessory protein UreG
- Pfam (hmmscan --cut_ga): cobW PF02492.26 (E=7e-37)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216368.1)
- Domains: Pfam-A via hmmscan --cut_ga — cobW (PF02492.26)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0378 - Curated reference: UniProt P9WFE3 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
13 functional partner(s); context anchor
ureF - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001965|Rv1852|ureG MATHSHPHSHTVPARPRRVRKPGEPLRIGVGGPVGSGKTALVAALCRQLRGELSLAVLTNDIYTTEDADFLRTHAVLPDDRIAAVQTGGCPHTAIRDDITANLDAIDELMAAHDALDLILVESGGDNLTATFSSGLVDAQIFVIDVAGGDKVPRKGGPGVTYSDLLVVNKTDLAALVGADLAVMARDADAVRDGRPTVLQSLTEDPAASDVVAWVRSQLAADGV