ureC Family assigned · medium auto-curated
H37Rv Rv1850 · MTBC0 mtbc0_001963 ·
577 aa · 2115999–2117732 (+) ·
RefSeq NP_216366.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | urease subunit alpha |
|---|---|
| MTBC0 PGAP re-annotation | urease subunit alpha |
| Revised (this work) | Urease subunit alpha. Pfam: Urease_alpha (PF00449.27), Amidohydro_1 (PF01979.27). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WFF1
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Urease subunit alpha |
| EC (curated) |
EC 3.5.1.5
|
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
E Amino acid transport and metabolism
|
|---|---|
| Preferred name | ureC |
| eggNOG description | Belongs to the metallo-dependent hydrolases superfamily. Urease alpha subunit family |
| Orthologous group | COG0804 |
| EC number |
EC 3.5.1.5
|
| KEGG orthology |
K01428
|
| KEGG pathways |
map00220, map00230, map00791, map01100, map01120, map05120
|
| Gene Ontology (2) |
GO:0008150, GO:0040007
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.392 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 8 synonymous, 9 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Urease_alpha | PF00449.27 | 3.7e-51 | 3–126 | Urease alpha-subunit, N-terminal domain |
Amidohydro_1 | PF01979.27 | 1.1e-70 | 132–459 | Amidohydrolase family |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: ureB (urease subunit beta), high confidence from genomic context alone (score 1000 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1849 ureB |
urease subunit beta | 999 | 1000 ctx | neighborhood:882 fusion:706 cooccurence:774 coexpression:883 experimental:928 database:900 textmining:707 |
Rv1848 ureA |
urease subunit gamma | 999 | 1000 ctx | neighborhood:882 fusion:900 cooccurence:774 coexpression:860 experimental:928 database:900 |
Rv1852 ureG |
urease accessory protein UreG | 997 | 991 ctx | neighborhood:874 cooccurence:774 coexpression:722 textmining:692 |
Rv1851 ureF |
urease accessory protein UreF | 996 | 991 ctx | neighborhood:882 coexpression:887 textmining:625 |
Rv1853 ureD |
urease accessory protein UreD | 961 | 925 ctx | neighborhood:874 coexpression:431 textmining:513 |
Rv1847 |
esterase | 804 | 805 ctx | neighborhood:804 |
Rv3151 nuoG |
NADH-quinone oxidoreductase subunit G | 612 | 65 | textmining:603 |
Rv0198c zmp1 |
zinc metalloprotease | 450 | 57 | textmining:441 |
Rv1392 metK |
S-adenosylmethionine synthetase | 640 | 55 | textmining:635 |
Rv2605c tesB2 |
acyl-CoA thioesterase II | 661 | 47 | textmining:659 |
Rv1326c glgB |
1,4-alpha-glucan branching protein | 531 | 47 | textmining:528 |
Rv1886c fbpB |
diacylglycerol acyltransferase/mycolyltransferase Ag85B | 531 | 47 | textmining:529 |
Rv3859c gltB |
glutamate synthase large subunit | 518 | 46 | textmining:516 |
Rv2604c snoP |
glutamine amidotransferase SnoP | 517 | 46 | textmining:515 |
Rv1980c mpt64 |
immunogenic protein Mpt64 | 425 | 46 | textmining:422 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: urease subunit alpha
- MTBC0 PGAP product: urease subunit alpha
- Pfam (hmmscan --cut_ga): Urease_alpha PF00449.27 (E=4e-51), Amidohydro_1 PF01979.27 (E=1e-70)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216366.1)
- Domains: Pfam-A via hmmscan --cut_ga — Urease_alpha (PF00449.27), Amidohydro_1 (PF01979.27)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0804 - Curated reference: UniProt P9WFF1 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
20 functional partner(s); context anchor
ureB - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001963|Rv1850|ureC MARLSRERYAQLYGPTTGDRIRLADTNLLVEVTEDRCGGPGLAGDEAVFGGGKVLRESMGQGRASRADGAPDTVITGAVIIDYWGIIKADIGIRDGRIVGIGKAGNPDIMTGVHRDLVVGPSTEIISGNRRIVTAGTVDCHVHLICPQIIVEALAAGTTTIIGGGTGPAEGTKATTVTPGEWHLARMLESLDGWPVNFALLGKGNTVNPDALWEQLRGGASGFKLHEDWGSTPAAIDTCLAVADVAGVQVALHSDTLNETGFVEDTIGAIAGRSIHAYHTEGAGGGHAPDIITVAAQPNVLPSSTNPTRPHTVNTLDEHLDMLMVCHHLNPRIPEDLAFAESRIRPSTIAAEDVLHDMGAISMIGSDSQAMGRVGEVVLRTWQTAHVMKARRGALEGDPSGSQAADNNRVRRYIAKYTICPAIAHGMDHLIGSVEVGKLADLVLWEPAFFGVRPHVVLKGGAIAWAAMGDANASIPTPQPVLPRPMFGAAAATAAATSVHFVAPQSIDARLADRLAVNRGLAPVADVRAVGKTDLPLNDALPSIEVDPDTFTVRIDGQVWQPQPAAELPMTQRYFLF