blaR Family assigned · medium auto-curated
H37Rv Rv1845c · MTBC0 mtbc0_001958 ·
316 aa · 2113256–2114206 (-) ·
RefSeq NP_216361.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | sensor-transducer protein BlaR |
|---|---|
| MTBC0 PGAP re-annotation | M56 family metallopeptidase |
| Revised (this work) | M56 family metallopeptidase. Pfam: Peptidase_M48 (PF01435.25). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P95164
TrEMBL · unreviewed
· Inferred from homology
|
|---|---|
| UniProt name | Possible sensor-transducer protein BlaR |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
M Cell wall / membrane / envelope biogenesis
|
|---|---|
| eggNOG description | Zn-dependent protease with chaperone function |
| Orthologous group | COG0501 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.321 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 4 synonymous, 3 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Peptidase_M48 | PF01435.25 | 7.4e-08 | 151–201 | Peptidase family M48 |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: blaI (transcriptional repressor BlaI), high confidence from genomic context alone (score 976 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1846c blaI |
transcriptional repressor BlaI | 976 | 976 ctx | neighborhood:869 cooccurence:772 |
Rv1844c gnd1 |
6-phosphogluconate dehydrogenase | 848 | 848 ctx | neighborhood:841 |
Rv1843c guaB1 |
inosine-5'-monophosphate dehydrogenase | 826 | 819 ctx | neighborhood:802 |
Rv1842c hyp |
hypothetical protein | 716 | 716 ctx | neighborhood:700 |
Rv1841c hyp |
hypothetical protein | 712 | 713 ctx | neighborhood:700 |
Rv0342 iniA |
isoniazid inductible protein IniA | 663 | 644 | database:643 |
Rv2001 hyp |
hypothetical protein | 583 | 584 ctx | cooccurence:583 |
Rv2673 aftC |
alpha-(1->3)-arabinofuranosyltransferase | 553 | 553 ctx | cooccurence:553 |
Rv1274 lprB |
lipoprotein LprB | 564 | 537 ctx | cooccurence:531 |
Rv0184 hyp |
hypothetical protein | 531 | 532 ctx | cooccurence:528 |
Rv1837c glcB |
malate synthase | 516 | 516 ctx | neighborhood:505 |
Rv0495c hyp |
hypothetical protein | 515 | 515 ctx | cooccurence:504 |
Rv2342 hyp |
hypothetical protein | 505 | 505 ctx | cooccurence:498 |
Rv3346c |
transmembrane protein | 502 | 503 ctx | cooccurence:500 |
Rv0466 hyp |
hypothetical protein | 492 | 493 ctx | cooccurence:490 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: sensor-transducer protein BlaR
- MTBC0 PGAP product: M56 family metallopeptidase
- Pfam (hmmscan --cut_ga): Peptidase_M48 PF01435.25 (E=7e-08)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216361.1)
- Domains: Pfam-A via hmmscan --cut_ga — Peptidase_M48 (PF01435.25)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0501 - Curated reference: UniProt P95164 (TrEMBL, unreviewed; Inferred from homology)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
38 functional partner(s); context anchor
blaI - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001958|Rv1845c|blaR MSALAFTILAVLLAGPTPALLARATWPLRAPRAAMVLWQAIALAAVLSSFSAGIAIASRLLMPGPDGRPTTSFVGAAGRLGWPLWAAYITVFALTVLVGARLAVAVVRVATATRRRRAHHRMVVDLVGVGHNGALAQPCARARDLRVLDVAQPLAYCLPGVRSRVVVSEGTLTALADAEVAAILTHERAHLRARHDLVLEAFTAVHAAFPRLVRSANALGAVQLLVELLADDAAVRAAGRTPLARALVACASGRAPSGALAVGGPSTVLRVRRLSGRGNSAVLSAAAYLAAAAVLVVPTVALAVPWLTQLQRLFIA