modC Family assigned · medium auto-curated
H37Rv Rv1859 · MTBC0 mtbc0_001972 ·
369 aa · 2124612–2125721 (+) ·
RefSeq NP_216375.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | molybdenum ABC transporter ATP-binding protein ModC |
|---|---|
| MTBC0 PGAP re-annotation | sulfate/molybdate ABC transporter ATP-binding protein |
| Revised (this work) | Sulfate/molybdate ABC transporter ATP-binding protein. Pfam: ABC_tran (PF00005.34), TOBE (PF03459.24). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WQL3
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Molybdenum import ATP-binding protein ModC |
| EC (curated) |
EC 7.3.2.5
|
| Curated function | Part of the ABC transporter complex ModABC involved in molybdenum import. Responsible for energy coupling to the transport system. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
P Inorganic ion transport and metabolism
|
|---|---|
| Preferred name | modC |
| eggNOG description | Part of the ABC transporter complex ModABC involved in molybdenum import. Responsible for energy coupling to the transport system |
| Orthologous group | COG1118 |
| EC number |
EC 3.6.3.29
|
| KEGG orthology |
K02017
|
| KEGG pathways |
map02010
|
| KEGG modules |
M00189
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.454 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 5 synonymous, 6 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
ABC_tran | PF00005.34 | 1.1e-33 | 16–166 | ABC transporter |
TOBE | PF03459.24 | 1.3e-14 | 295–359 | TOBE domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: modB (molybdenum ABC transporter permease ModB), high confidence from genomic context alone (score 1000 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1858 modB |
molybdenum ABC transporter permease ModB | 999 | 1000 ctx | neighborhood:816 fusion:891 cooccurence:599 coexpression:662 database:900 |
Rv1286 cysC |
adenylyl-sulfate kinase | 991 | 987 | coexpression:984 textmining:418 |
Rv1857 modA |
molybdate ABC transporter substrate-binding lipoprotein ModA | 987 | 984 ctx | neighborhood:882 database:800 |
Rv2398c cysW |
sulfate ABC transporter permease CysW | 878 | 861 | coexpression:724 |
Rv2399c cysT |
sulfate ABC transporter permease CysT | 830 | 808 | coexpression:725 |
Rv1860 apa hyp |
hypothetical protein | 801 | 797 ctx | neighborhood:796 |
Rv2400c subI |
sulfate ABC transporter substrate-binding lipoprotein SubI | 771 | 741 | coexpression:688 |
Rv2064 cobG |
precorrin-3B synthase | 709 | 682 | coexpression:671 |
Rv2391 sirA |
sulfite reductase | 708 | 680 | coexpression:669 |
Rv1862 adhA |
alcohol dehydrogenase A | 550 | 551 ctx | neighborhood:544 |
Rv1856c |
oxidoreductase | 550 | 550 ctx | neighborhood:543 |
Rv1861 |
transmembrane protein | 546 | 546 ctx | neighborhood:544 |
Rv2392 cysH |
phosphoadenosine phosphosulfate reductase | 559 | 519 | coexpression:502 |
Rv1285 cysD |
sulfate adenylyltransferase subunit 2 | 559 | 518 | coexpression:501 |
Rv1855c |
oxidoreductase | 464 | 463 ctx | neighborhood:450 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: molybdenum ABC transporter ATP-binding protein ModC
- MTBC0 PGAP product: sulfate/molybdate ABC transporter ATP-binding protein
- Pfam (hmmscan --cut_ga): ABC_tran PF00005.34 (E=1e-33), TOBE PF03459.24 (E=1e-14)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216375.1)
- Domains: Pfam-A via hmmscan --cut_ga — ABC_tran (PF00005.34), TOBE (PF03459.24)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1118 - Curated reference: UniProt P9WQL3 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
31 functional partner(s); context anchor
modB - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001972|Rv1859|modC MSKLQLRAVVADRRLDVEFSVSAGEVLAVLGPNGAGKSTALHVIAGLLRPDAGLVRLGDRVLTDTEAGVNVATHDRRVGLLLQDPLLFPHLSVAKNVAFGPQCRRGMFGSGRARTRASALRWLREVNAEQFADRKPRQLSGGQAQRVAIARALAAEPDVLLLDEPLTGLDVAAAAGIRSVLRSVVARSGCAVVLTTHDLLDVFTLADRVLVLESGTIAEIGPVADVLTAPRSRFGARIAGVNLVNGTIGPDGSLRTQSGAHWYGTPVQDLPTGHEAIAVFPPTAVAVYPEPPHGSPRNIVGLTVAEVDTRGPTVLVRGHDQPGGAPGLAACITVDAATELRVAPGSRVWFSVKAQEVALHPAPHQHASS