Rv1573 Still unknown · low auto-curated
H37Rv Rv1573 · MTBC0 mtbc0_001680 ·
136 aa · 1791278–1791688 (+) ·
RefSeq NP_216089.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | phage protein |
|---|---|
| MTBC0 PGAP re-annotation | hypothetical protein |
| Revised (this work) | Conserved hypothetical protein; no recognised domain. Function unknown. |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
O06617
TrEMBL · unreviewed
· Predicted
|
|---|---|
| UniProt name | Probable PhiRv1 phage protein |
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.333 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 1 synonymous, 1 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
No Pfam-A domain above the gathering threshold (or not yet scanned).
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv1572c (Conserved hypothetical protein; Rv1572c, (MTCY336.31B), len: 34 aa. Partial ORF,part of REP13E12 repeat element; 3' end of Rv1587c (MTCY336.), medium confidence from genomic context alone (score 570 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1572c |
Conserved hypothetical protein; Rv1572c, (MTCY336.31B), len: 34 aa. Partial ORF,part of REP13E12 repeat element; 3' end of Rv1587c (MTCY336. | 844 | 570 ctx | neighborhood:569 textmining:652 |
Rv1574 |
phage protein | 471 | 471 ctx | neighborhood:441 |
Rv1575 |
phage protein | 467 | 467 ctx | neighborhood:441 |
Rv3469c mhpE |
4-hydroxy-2-oxovalerate aldolase MhpE | 673 | 89 | textmining:656 |
Rv2645 hyp |
hypothetical protein | 630 | 55 | textmining:625 |
Rv3470c ilvB2 |
acetolactate synthase large subunit | 630 | 55 | textmining:625 |
Rv1585c |
phage protein | 547 | 55 | textmining:541 |
Rv2650c |
prophage protein | 805 | 53 | textmining:803 |
Rv2290 lppO |
lipoprotein LppO | 523 | 51 | textmining:518 |
Rv1584c |
phage protein | 517 | 47 | textmining:514 |
Rv2291 sseB |
thiosulfate sulfurtransferase SseB | 514 | 47 | textmining:511 |
Rv3669 |
transmembrane protein | 803 | 41 | textmining:803 |
Rv2659c |
prophage integrase | 510 | 41 | textmining:510 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: phage protein
- MTBC0 PGAP product: hypothetical protein
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216089.1)
- Domains: Pfam-A via hmmscan --cut_ga — none above threshold
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Curated reference: UniProt O06617 (TrEMBL, unreviewed; Predicted)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
13 functional partner(s); context anchor
Rv1572c - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001680|Rv1573| MTTTPARFNHLVTVTDLETGDRAVCDRDQVAETIRAWFPDAPLEVREALVRLQAALNRHEHTGELEAFLRISVEHADAAGGDECGPAILAGRSGPEQAAINRQLGLAGDDEPDGDDTPPWSRMIGLGGGSPAEDER