sseB Resolved · high auto-curated
H37Rv Rv2291 · MTBC0 mtbc0_002432 ·
284 aa · 2587921–2588775 (+) ·
RefSeq NP_216807.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | thiosulfate sulfurtransferase SseB |
|---|---|
| MTBC0 PGAP re-annotation | sulfurtransferase |
| Revised (this work) | Sulfurtransferase. Pfam: Rhodanese (PF00581.26). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WHF5
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Putative thiosulfate sulfurtransferase SseB |
| EC (curated) |
EC 2.8.1.1
|
UniProt still lists this protein as Putative thiosulfate sulfurtransferase SseB; the revised annotation above is ahead of the current UniProt record.
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
P Inorganic ion transport and metabolism
|
|---|---|
| Preferred name | sseB |
| eggNOG description | sulfurtransferase |
| Orthologous group | COG2897 |
| EC number |
EC 2.8.1.1, EC 2.8.1.2
|
| KEGG orthology |
K01011
|
| KEGG pathways |
map00270, map00920, map01100, map01120, map04122
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains) pseudogene candidate
| pN/pS | 0.739 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 3 synonymous, 5 missense, 1 nonsense, 2 frameshift |
| Disruption | 3 distinct premature-stop/frameshift site(s); most common in 5.23% of strains (7589) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Rhodanese | PF00581.26 | 2.6e-16 | 10–131 | Rhodanese-like domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: lppO (lipoprotein LppO), medium confidence from genomic context alone (score 649 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3283 sseA |
thiosulfate sulfurtransferase SseA | 941 | 928 | database:900 |
Rv0815c cysA2 |
thiosulfate sulfurtransferase CysA | 936 | 928 | database:900 |
Rv3025c iscS |
cysteine desulfurase | 923 | 915 | database:900 |
Rv2391 sirA |
sulfite reductase | 909 | 904 | database:900 |
Rv2392 cysH |
phosphoadenosine phosphosulfate reductase | 909 | 904 | database:900 |
Rv0075 |
aminotransferase | 903 | 903 | database:900 |
Rv2294 |
cystathionine beta-lyase | 905 | 902 | database:900 |
Rv2334 cysK1 |
O-acetylserine sulfhydrylase | 869 | 818 | database:800 |
Rv3684 |
lyase | 827 | 818 | database:800 |
Rv1079 metB |
cystathionine gamma-synthase | 867 | 817 | database:800 |
Rv0391 metZ |
O-succinylhomoserine sulfhydrylase | 866 | 816 | database:800 |
Rv3238c mddA |
integral membrane protein | 806 | 806 | database:800 |
Rv2290 lppO |
lipoprotein LppO | 945 | 649 ctx | neighborhood:644 textmining:852 |
Rv3673c |
membrane-anchored thioredoxin-like protein | 631 | 630 | database:561 |
Rv0526 |
thioredoxin | 631 | 630 | database:561 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: thiosulfate sulfurtransferase SseB
- MTBC0 PGAP product: sulfurtransferase
- Pfam (hmmscan --cut_ga): Rhodanese PF00581.26 (E=3e-16)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216807.1)
- Domains: Pfam-A via hmmscan --cut_ga — Rhodanese (PF00581.26)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG2897 - Curated reference: UniProt P9WHF5 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
47 functional partner(s); context anchor
lppO - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_002432|Rv2291|sseB MQARGQVLITAAELAGMIQAGDPVSILDVRWRLDEPDGHAAYLQGHLPGAVFVSLEDELSDHTIAGRGRHPLPSGASLQATVRRCGIRHDVPVVVYDDWNRAGSARAWWVLTAAGIANVRILDGGLPAWRSAGGSIETGQVSPQLGNVTVLHDDLYAGQRLTLTAQQAGAGGVTLLDARVPERFRGDVEPVDAVAGHIPGAINVPSGSVLADDGTFLGNGALNALLSDHGIDHGGRVGVYCGSGVSAAVIVAALAVIGQDAALFPGSWSEWSSDPTRPVGRGTA