Rv1565c Family assigned · medium auto-curated
H37Rv Rv1565c · MTBC0 mtbc0_001673 ·
729 aa · 1783604–1785793 (-) ·
RefSeq NP_216081.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | acyltransferase |
|---|---|
| MTBC0 PGAP re-annotation | acyltransferase family protein |
| Revised (this work) | Acyltransferase family protein. Pfam: Acyl_transf_3 (PF01757.29), SGNH (PF19040.7). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
O06625
TrEMBL · unreviewed
· Predicted
|
|---|---|
| UniProt name | Conserved hypothetical membrane protein |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
I Lipid transport and metabolism
|
|---|---|
| eggNOG description | Acyltransferase family |
| Orthologous group | COG1835 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.403 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 8 synonymous, 9 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Acyl_transf_3 | PF01757.29 | 1.4e-21 | 33–377 | Acyltransferase domain |
SGNH | PF19040.7 | 3.5e-22 | 500–716 | SGNH domain (fused to AT3 domains) |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: treX (maltooligosyl trehalose synthase), high confidence from genomic context alone (score 837 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1564c treX |
maltooligosyl trehalose synthase | 843 | 837 ctx | neighborhood:827 |
Rv1563c treY |
maltooligosyl trehalose synthase | 830 | 830 ctx | neighborhood:827 |
Rv1562c treZ |
malto-oligosyltrehalose trehalohydrolase | 829 | 829 ctx | neighborhood:827 |
Rv1566c ripD hyp |
hypothetical protein | 785 | 778 ctx | neighborhood:774 |
Rv1489A hyp |
hypothetical protein | 527 | 528 ctx | cooccurence:525 |
Rv0472c |
HTH-type transcriptional regulator | 491 | 492 ctx | cooccurence:476 |
Rv1354c hyp |
hypothetical protein | 453 | 452 | coexpression:421 |
Rv3668c |
protease | 452 | 452 ctx | cooccurence:442 |
Rv1275 lprC |
lipoprotein LprC | 449 | 449 ctx | cooccurence:446 |
Rv1754c hyp |
hypothetical protein | 447 | 447 ctx | cooccurence:444 |
Rv1568 bioA |
adenosylmethionine--8-amino-7-oxononanoate aminotransferase BioA | 439 | 439 ctx | neighborhood:439 |
Rv1510 hyp |
hypothetical protein | 463 | 429 | |
Rv1814 erg3 |
membrane-bound C-5 sterol desaturase | 419 | 420 | coexpression:401 |
Rv3630 |
integral membrane protein | 446 | 415 | |
Rv2972c hyp |
hypothetical protein | 446 | 411 | coexpression:410 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: acyltransferase
- MTBC0 PGAP product: acyltransferase family protein
- Pfam (hmmscan --cut_ga): Acyl_transf_3 PF01757.29 (E=1e-21), SGNH PF19040.7 (E=4e-22)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216081.1)
- Domains: Pfam-A via hmmscan --cut_ga — Acyl_transf_3 (PF01757.29), SGNH (PF19040.7)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1835 - Curated reference: UniProt O06625 (TrEMBL, unreviewed; Predicted)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
21 functional partner(s); context anchor
treX - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001673|Rv1565c| MLTLSPPRPPALTPEPALPPVTMGTRTTGFYRHDLDGLRGVAIALVAVFHVWFGRVSGGVDVFLALSGFFFGGKILRAALNPDLSLSPIAEVIRLIRRLLPALVVVLAGCALLTIAIQPQTRWEAFANQSLASLGYYQNWELASTVSNYLRAGEAVSPLQHIWSMSVQGQFYLAFLLLVAGCAYLLRRLFRGPRAPYLRTMFVVLLSTLTLASFIYAIVAHHAYQATAYYNTFARAWELLAGALVGAVVPHVRWPMWLRTAVATAALAAILSCGALIDGVKEFPGPWALVPVGATMLMILAGANRQGHPGTRDRLPLPNRLLATAPLVALGAMAYSWYLWHWPLLIFWLSYTGHRHANFVEGAAVLLVSGLLAYLTTRLVEDPLRYRAPAGVRSPAAVPPIPWRLRLRRPTIVLGSVVALLGVALTATSFTWREHVIVQRAAGKELSGLSSRDYPGARALIDHVRVPKLRMRPTVLEVRHDLPTSTKDGCISDFVNPAIINCTYGDVDAPRTIALAGGSHAEHWLTALDLLGRMHHFKVVTYLKMGCPLSTEEVPLIMGNNAPYPQCHQWVQAAMAKLVADHPDYVFTTSTRPWNIKPGDVMPATYVGIWQTFADNNIPVLAMRDTPWLVKDGQPFIPADCLAKGGNPQSCGIARSKVLVDRNPTLDFVARFPLLKPLDMSDAICRTDTCRAVEGNVLVYRDSHHLTPTYMRTMTSELGRQIAANTDWW