fusA2 Family assigned · medium auto-curated
H37Rv Rv0120c · MTBC0 mtbc0_000131 ·
714 aa · 145793–147937 (-) ·
RefSeq NP_214634.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | elongation factor G |
|---|---|
| MTBC0 PGAP re-annotation | elongation factor G-like protein EF-G2 |
| Revised (this work) | Elongation factor G-like protein EF-G2. Pfam: GTP_EFTU (PF00009.34), MMR_HSR1 (PF01926.30), EF-G_D2 (PF22042.3), GTP_EFTU_D2 (PF03144.32), EFG_III (PF14492.13), EFG_IV (PF03764.25), EFG_C (PF00679.31). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WNM9
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Elongation factor G-like protein |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
J Translation, ribosomal structure and biogenesis
|
|---|---|
| Preferred name | fusA2 |
| eggNOG description | elongation factor G |
| Orthologous group | COG0480 |
| KEGG orthology |
K02355
|
| Gene Ontology (13) |
GO:0005575, GO:0005618, GO:0005623, GO:0005886, GO:0006790, GO:0008150, GO:0008152, GO:0009987, GO:0016020, GO:0030312, GO:0044237, GO:0044464 +1 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains) pseudogene candidate
| pN/pS | 0.349 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 12 synonymous, 12 missense, 0 nonsense, 2 frameshift |
| Disruption | 2 distinct premature-stop/frameshift site(s); most common in 5.27% of strains (7654) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
GTP_EFTU | PF00009.34 | 1.2e-38 | 23–286 | Elongation factor Tu GTP binding domain |
MMR_HSR1 | PF01926.30 | 1.7e-06 | 25–149 | 50S ribosome-binding GTPase |
EF-G_D2 | PF22042.3 | 9.9e-09 | 317–413 | Elongation factor G domain 2 |
GTP_EFTU_D2 | PF03144.32 | 9.1e-10 | 329–411 | Elongation factor Tu domain 2 |
EFG_III | PF14492.13 | 5.4e-23 | 425–497 | Elongation Factor G, domain III |
EFG_IV | PF03764.25 | 3.1e-41 | 501–617 | Elongation factor G, domain IV |
EFG_C | PF00679.31 | 4.2e-23 | 622–707 | Elongation factor G C-terminus |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: rplC (50S ribosomal protein L3), high confidence from genomic context alone (score 981 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0701 rplC |
50S ribosomal protein L3 | 983 | 981 ctx | cooccurence:438 coexpression:730 experimental:829 |
Rv0721 rpsE |
30S ribosomal protein S5 | 983 | 981 ctx | cooccurence:435 coexpression:731 experimental:829 |
Rv0704 rplB |
50S ribosomal protein L2 | 983 | 981 ctx | cooccurence:441 coexpression:729 experimental:829 |
Rv0683 rpsG |
30S ribosomal protein S7 | 982 | 980 | coexpression:730 experimental:829 |
Rv0702 rplD |
50S ribosomal protein L4 | 982 | 980 ctx | cooccurence:408 coexpression:729 experimental:829 |
Rv0716 rplE |
50S ribosomal protein L5 | 981 | 979 | coexpression:729 experimental:829 |
Rv0708 rplP |
50S ribosomal protein L16 | 981 | 979 | coexpression:730 experimental:829 |
Rv0714 rplN |
50S ribosomal protein L14 | 980 | 978 | coexpression:729 experimental:829 |
Rv0682 rpsL |
30S ribosomal protein S12 | 980 | 978 | coexpression:728 experimental:829 |
Rv3443c rplM |
50S ribosomal protein L13 | 979 | 977 | coexpression:732 experimental:829 |
Rv3459c rpsK |
30S ribosomal protein S11 | 979 | 977 | coexpression:731 experimental:829 |
Rv0705 rpsS |
30S ribosomal protein S19 | 978 | 976 | coexpression:729 experimental:829 |
Rv0706 rplV |
50S ribosomal protein L22 | 978 | 976 | coexpression:729 experimental:829 |
Rv0719 rplF |
50S ribosomal protein L6 | 977 | 975 | coexpression:728 experimental:829 |
Rv0700 rpsJ |
30S ribosomal protein S10 | 977 | 975 | coexpression:730 experimental:829 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: elongation factor G
- MTBC0 PGAP product: elongation factor G-like protein EF-G2
- Pfam (hmmscan --cut_ga): GTP_EFTU PF00009.34 (E=1e-38), MMR_HSR1 PF01926.30 (E=2e-06), EF-G_D2 PF22042.3 (E=1e-08), GTP_EFTU_D2 PF03144.32 (E=9e-10), EFG_III PF14492.13 (E=5e-23), EFG_IV PF03764.25 (E=3e-41), EFG_C PF00679.31 (E=4e-23)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_214634.1)
- Domains: Pfam-A via hmmscan --cut_ga — GTP_EFTU (PF00009.34), MMR_HSR1 (PF01926.30), EF-G_D2 (PF22042.3), GTP_EFTU_D2 (PF03144.32), EFG_III (PF14492.13), EFG_IV (PF03764.25), EFG_C (PF00679.31)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0480 - Curated reference: UniProt P9WNM9 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
239 functional partner(s); context anchor
rplC - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_000131|Rv0120c|fusA2 MADRVNASQGAAAAPTANGPGGVRNVVLVGPSGGGKTTLIEALLVAAKVLSRPGSVTEGTTVCDFDEAEIRQQRSVGLAVASLAYDGIKVNLVDTPGYADFVGELRAGLRAADCALFVIAANEGVDEPTKSLWQECSQVGMPRAVVITKLDHARANYREALTAAQDAFGDKVLPLYLPSGDGLIGLLSQALYEYADGKRTTRTPAESDTERIEEARGALIEGIIEESEDESLMERYLGGETIDESVLIQDLEKAVARGSFFPVIPVCSSTGVGTLELLEVATRGFPSPMEHPLPEVFTPQGVPHAELACDNDAPLLAEVVKTTSDPYVGRVSLVRVFSGTIRPDTTVHVSGHFSSFFGGGTSNTHPDHDEDERIGVLSFPLGKQQRPAAAVVAGDICAIGKLSRAETGDTLSDKAEPLVLKPWTMPEPLLPIAIAAHAKTDEDKLSVGLGRLAAEDPTLRIEQNQETHQVVLWCMGEAHAGVVLDTLANRYGVSVDTIELRVPLRETFAGNAKGHGRHIKQSGGHGQYGVCDIEVEPLPEGSGFEFLDKVVGGAVPRQFIPSVEKGVRAQMDKGVHAGYPVVDIRVTLLDGKAHSVDSSDFAFQMAGALALREAAAATKVILLEPIDEISVLVPDDFVGAVLGDLSSRRGRVLGTETAGHDRTVIKAEVPQVELTRYAIDLRSLAHGAASFTRSFARYEPMPESAAARVKAGAG