rpsE Resolved · high auto-curated
H37Rv Rv0721 · MTBC0 mtbc0_000763 ·
220 aa · 818521–819183 (+) ·
RefSeq NP_215235.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | 30S ribosomal protein S5 |
|---|---|
| MTBC0 PGAP re-annotation | 30S ribosomal protein S5 |
| Revised (this work) | 30S ribosomal protein S5. Pfam: Ribosomal_S5 (PF00333.27), Ribosomal_S5_C (PF03719.22). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WH33
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Small ribosomal subunit protein uS5 |
| Curated function | With S4 and S12 plays an important role in translational accuracy..; FUNCTION: Located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
J Translation, ribosomal structure and biogenesis
|
|---|---|
| Preferred name | rpsE |
| eggNOG description | Located at the back of the 30S subunit body where it stabilizes the conformation of the head with respect to the body |
| Orthologous group | COG0098 |
| KEGG orthology |
K02988
|
| KEGG pathways |
map03010
|
| KEGG modules |
M00178, M00179
|
| Gene Ontology (55) |
GO:0003674, GO:0003735, GO:0005198, GO:0005575, GO:0005622, GO:0005623, GO:0005737, GO:0005829, GO:0005840, GO:0005886, GO:0006412, GO:0006518 +43 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 1.079 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 1 synonymous, 3 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Ribosomal_S5 | PF00333.27 | 7.4e-28 | 44–106 | Ribosomal protein S5, N-terminal domain |
Ribosomal_S5_C | PF03719.22 | 3.6e-28 | 119–188 | Ribosomal protein S5, C-terminal domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: rpsM (30S ribosomal protein S13), high confidence from genomic context alone (score 1000 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3460c rpsM |
30S ribosomal protein S13 | 999 | 1000 ctx | cooccurence:649 coexpression:751 experimental:999 database:662 textmining:597 |
Rv1642 rpmI |
50S ribosomal protein L35 | 999 | 1000 | coexpression:888 experimental:999 |
Rv2909c rpsP |
30S ribosomal protein S16 | 999 | 1000 | coexpression:834 experimental:999 database:844 |
Rv0701 rplC |
50S ribosomal protein L3 | 999 | 1000 ctx | cooccurence:561 coexpression:968 experimental:999 textmining:591 |
Rv0714 rplN |
50S ribosomal protein L14 | 999 | 1000 ctx | neighborhood:714 cooccurence:718 coexpression:874 experimental:999 textmining:592 |
Rv0709 rpmC |
50S ribosomal protein L29 | 999 | 1000 | coexpression:973 experimental:999 |
Rv0710 rpsQ |
30S ribosomal protein S17 | 999 | 1000 ctx | cooccurence:463 coexpression:967 experimental:999 database:925 |
Rv0715 rplX |
50S ribosomal protein L24 | 999 | 1000 ctx | neighborhood:714 coexpression:923 experimental:999 |
Rv2785c rpsO |
30S ribosomal protein S15 | 999 | 1000 | coexpression:853 experimental:999 database:925 |
Rv0716 rplE |
50S ribosomal protein L5 | 999 | 1000 ctx | neighborhood:714 cooccurence:758 coexpression:897 experimental:999 textmining:612 |
Rv3461c rpmJ |
50S ribosomal protein L36 | 999 | 1000 | coexpression:865 experimental:999 |
Rv0705 rpsS |
30S ribosomal protein S19 | 999 | 1000 ctx | cooccurence:549 coexpression:969 experimental:999 database:925 textmining:598 |
Rv0708 rplP |
50S ribosomal protein L16 | 999 | 1000 ctx | cooccurence:523 coexpression:973 experimental:999 textmining:606 |
Rv0053 rpsF |
30S ribosomal protein S6 | 999 | 1000 | coexpression:861 experimental:999 database:844 |
Rv0718 rpsH |
30S ribosomal protein S8 | 999 | 1000 ctx | neighborhood:825 fusion:671 cooccurence:720 coexpression:968 experimental:999 database:925 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: 30S ribosomal protein S5
- MTBC0 PGAP product: 30S ribosomal protein S5
- Pfam (hmmscan --cut_ga): Ribosomal_S5 PF00333.27 (E=7e-28), Ribosomal_S5_C PF03719.22 (E=4e-28)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215235.1)
- Domains: Pfam-A via hmmscan --cut_ga — Ribosomal_S5 (PF00333.27), Ribosomal_S5_C (PF03719.22)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0098 - Curated reference: UniProt P9WH33 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
263 functional partner(s); context anchor
rpsM - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_000763|Rv0721|rpsE MAEQPAGQAGTTDNRDARGDREGRRRDSGRGSRERDGEKSNYLERVVAINRVSKVVKGGRRFSFTALVIVGDGNGMVGVGYGKAKEVPAAIAKGVEEARKSFFRVPLIGGTITHPVQGEAAAGVVLLRPASPGTGVIAGGAARAVLECAGVHDILAKSLGSDNAINVVHATVAALKLLQRPEEVAARRGLPIEDVAPAGMLKARRKSEALAASVLPDRTI