mak Resolved · high auto-curated
H37Rv Rv0127 · MTBC0 mtbc0_000138 ·
455 aa · 154578–155945 (+) ·
RefSeq NP_214641.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | maltokinase |
|---|---|
| MTBC0 PGAP re-annotation | maltokinase |
| Revised (this work) | Maltokinase. Pfam: Mak_N_cap (PF18085.7). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
O07177
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Maltokinase |
| EC (curated) |
EC 2.7.1.175
|
| Curated function | Catalyzes the ATP-dependent phosphorylation of maltose to maltose 1-phosphate (By similarity). Is involved in a branched alpha-glucan biosynthetic pathway from trehalose, together with TreS, GlgE and GlgB. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
G Carbohydrate transport and metabolism
|
|---|---|
| Preferred name | mak |
| eggNOG description | Catalyzes the ATP-dependent phosphorylation of maltose to maltose 1-phosphate. Is involved in a branched alpha-glucan biosynthetic pathway from trehalose, together with TreS, GlgE and GlgB (By similarity) |
| Orthologous group | COG3281 |
| EC number |
EC 2.7.1.175
|
| KEGG orthology |
K16146
|
| KEGG pathways |
map00500, map01100
|
| Gene Ontology (35) |
GO:0005575, GO:0005622, GO:0005623, GO:0005737, GO:0005829, GO:0005886, GO:0005975, GO:0005984, GO:0005991, GO:0005992, GO:0006793, GO:0006796 +23 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.474 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 6 synonymous, 8 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Mak_N_cap | PF18085.7 | 4.3e-14 | 16–96 | Maltokinase N-terminal cap domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: treS (trehalose synthase/amylase TreS), high confidence from genomic context alone (score 1000 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0126 treS |
trehalose synthase/amylase TreS | 999 | 1000 ctx | neighborhood:771 fusion:900 cooccurence:766 experimental:997 database:900 textmining:946 |
Rv2471 aglA |
alpha-glucosidase AglA | 994 | 994 ctx | fusion:834 experimental:484 database:900 |
Rv1327c glgE |
alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase | 999 | 990 ctx | cooccurence:771 experimental:484 database:900 textmining:965 |
Rv1326c glgB |
1,4-alpha-glucan branching protein | 997 | 949 ctx | fusion:899 textmining:955 |
Rv1781c malQ |
4-alpha-glucanotransferase | 973 | 947 | database:900 textmining:517 |
Rv1212c glgA |
capsular glucan synthase | 994 | 928 | database:900 textmining:933 |
Rv1562c treZ |
malto-oligosyltrehalose trehalohydrolase | 976 | 811 ctx | cooccurence:706 textmining:879 |
Rv1563c treY |
maltooligosyl trehalose synthase | 961 | 795 ctx | cooccurence:741 textmining:822 |
Rv0128 |
transmembrane protein | 795 | 795 ctx | neighborhood:794 |
Rv1564c treX |
maltooligosyl trehalose synthase | 900 | 754 ctx | cooccurence:538 coexpression:422 textmining:611 |
Rv0125 pepA |
serine protease PepA | 701 | 701 ctx | neighborhood:698 |
Rv3068c pgmA |
phosphoglucomutase PgmA | 556 | 537 ctx | fusion:499 |
Rv1328 glgP |
glycogen phosphorylase | 854 | 417 | textmining:760 |
Rv3372 otsB2 |
trehalose 6-phosphate phosphatase | 834 | 383 | textmining:743 |
Rv2006 otsB1 |
trehalose-6-phosphate phosphatase OtsB | 823 | 337 | textmining:745 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: maltokinase
- MTBC0 PGAP product: maltokinase
- Pfam (hmmscan --cut_ga): Mak_N_cap PF18085.7 (E=4e-14)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_214641.1)
- Domains: Pfam-A via hmmscan --cut_ga — Mak_N_cap (PF18085.7)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG3281 - Curated reference: UniProt O07177 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
23 functional partner(s); context anchor
treS - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_000138|Rv0127|mak MTRSDTLATKLPWSDWLPRQRWYAGRNRELATVKPGVVVALRHNLDLVLVDVTYTDGATERYQVLVGWDFEPASEYGTKAAIGVADDRTGFDALYDVAGPQFLLSLIVSSAVCGTSTGEVTFTREPDVELPFAAQPRVCDAEQSNTSVIFDRRAILKVFRRVSSGINPDIELNRVLTRAGNPHVARLLGAYQFGRPNRSPTDALAYALGMVTEYEANAAEGWAMATASVRDLFAEGDLYAHEVGGDFAGESYRLGEAVASVHATLADSLGTAQATFPVDRMLARLSSTVAVVPELREYAPTIEQQFQKLAAEAITVQRVHGDLHLGQVLRTPESWLLIDFEGEPGQPLDERRAPDSPLRDVAGVLRSFEYAAYGPLVDQATDKQLAARAREWVERNRAAFCDGYAVASGIDPRDSALLLGAYELDKAVYETGYETRHRPGWLPIPLRSIARLTAS