treS Resolved · high auto-curated
H37Rv Rv0126 · MTBC0 mtbc0_000137 ·
601 aa · 152670–154475 (+) ·
RefSeq NP_214640.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | trehalose synthase/amylase TreS |
|---|---|
| MTBC0 PGAP re-annotation | maltose alpha-D-glucosyltransferase |
| Revised (this work) | Maltose alpha-D-glucosyltransferase. Pfam: Alpha-amylase (PF00128.32), Malt_amylase_C (PF16657.12). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WQ19
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Trehalose synthase/amylase TreS |
| EC (curated) |
EC 3.2.1.1, EC 5.4.99.16
|
| Curated function | Catalyzes the reversible interconversion of maltose and trehalose by transglucosylation. Also displays amylase activity, catalyzing the endohydrolysis of (1->4)-alpha-D-glucosidic linkages in glycogen and maltooligosaccharides such as maltoheptaose, to produce maltose which then can be converted to trehalose. TreS plays a key role in the utilization of trehalose for the production of glycogen and alpha-glucan via the TreS-Pep2 branch involved in the biosynthesis of maltose-1-phosphate (M1P). Might also function as a sensor and/or regulator of trehalose levels within the cell. Thus, when trehal. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
G Carbohydrate transport and metabolism
|
|---|---|
| Preferred name | treS |
| eggNOG description | Trehalose synthase |
| Orthologous group | COG0366 |
| EC number |
EC 3.2.1.1, EC 5.4.99.16
|
| KEGG orthology |
K05343
|
| KEGG pathways |
map00500, map01100
|
| CAZy family |
GH13
|
| Gene Ontology (64) |
GO:0000023, GO:0000271, GO:0003674, GO:0003824, GO:0004553, GO:0004556, GO:0005488, GO:0005509, GO:0005575, GO:0005622, GO:0005623, GO:0005737 +52 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.069 · strong purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 9 synonymous, 2 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Alpha-amylase | PF00128.32 | 4.8e-92 | 52–499 | Alpha amylase, catalytic domain |
Malt_amylase_C | PF16657.12 | 4.7e-22 | 505–581 | Maltogenic Amylase, C-terminal domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: mak (maltokinase), high confidence from genomic context alone (score 1000 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0127 mak |
maltokinase | 999 | 1000 ctx | neighborhood:771 fusion:900 cooccurence:766 experimental:997 database:900 textmining:946 |
Rv1563c treY |
maltooligosyl trehalose synthase | 998 | 977 ctx | cooccurence:722 database:900 textmining:948 |
Rv1564c treX |
maltooligosyl trehalose synthase | 989 | 975 ctx | cooccurence:537 coexpression:428 database:900 textmining:593 |
Rv2006 otsB1 |
trehalose-6-phosphate phosphatase OtsB | 993 | 968 | coexpression:408 database:900 textmining:794 |
Rv3372 otsB2 |
trehalose 6-phosphate phosphatase | 991 | 960 | database:900 textmining:807 |
Rv1562c treZ |
malto-oligosyltrehalose trehalohydrolase | 988 | 948 | database:900 textmining:796 |
Rv1328 glgP |
glycogen phosphorylase | 979 | 947 | database:900 textmining:636 |
Rv1781c malQ |
4-alpha-glucanotransferase | 969 | 939 | database:900 textmining:514 |
Rv1326c glgB |
1,4-alpha-glucan branching protein | 997 | 935 | database:900 textmining:965 |
Rv2471 aglA |
alpha-glucosidase AglA | 936 | 935 | database:900 |
Rv2402 |
trehalase | 986 | 933 | database:900 textmining:810 |
Rv3401 |
glycosyl hydrolase | 918 | 913 | database:900 |
Rv3031 |
1,4-alpha-glucan-branching protein | 936 | 901 | database:900 |
Rv0125 pepA |
serine protease PepA | 806 | 797 ctx | neighborhood:768 |
Rv1327c glgE |
alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase | 988 | 788 ctx | cooccurence:751 textmining:949 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: trehalose synthase/amylase TreS
- MTBC0 PGAP product: maltose alpha-D-glucosyltransferase
- Pfam (hmmscan --cut_ga): Alpha-amylase PF00128.32 (E=5e-92), Malt_amylase_C PF16657.12 (E=5e-22)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_214640.1)
- Domains: Pfam-A via hmmscan --cut_ga — Alpha-amylase (PF00128.32), Malt_amylase_C (PF16657.12)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0366 - Curated reference: UniProt P9WQ19 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
46 functional partner(s); context anchor
mak - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_000137|Rv0126|treS MNEAEHSVEHPPVQGSHVEGGVVEHPDAKDFGSAAALPADPTWFKHAVFYEVLVRAFFDASADGSGDLRGLIDRLDYLQWLGIDCIWLPPFYDSPLRDGGYDIRDFYKVLPEFGTVDDFVALVDAAHRRGIRIITDLVMNHTSESHPWFQESRRDPDGPYGDYYVWSDTSERYTDARIIFVDTEESNWSFDPVRRQFYWHRFFSHQPDLNYDNPAVQEAMIDVIRFWLGLGIDGFRLDAVPYLFEREGTNCENLPETHAFLKRVRKVVDDEFPGRVLLAEANQWPGDVVEYFGDPNTGGDECHMAFHFPLMPRIFMAVRRESRFPISEIIAQTPPIPDMAQWGIFLRNHDELTLEMVTDEERDYMYAEYAKDPRMKANVGIRRRLAPLLDNDRNQIELFTALLLSLPGSPVLYYGDEIGMGDVIWLGDRDGVRIPMQWTPDRNAGFSTANPGRLYLPPSQDPVYGYQAVNVEAQRDTSTSLLNFTRTMLAVRRRHPAFAVGAFQELGGSNPSVLAYVRQVAGDDGDTVLCVNNLSRFPQPIELDLQQWTNYTPVELTGHVEFPRIGQVPYLLTLPGHGFYWFQLTTHEVGAPPTCGGERRL