Rv2731 Still unknown · low auto-curated
H37Rv Rv2731 · MTBC0 mtbc0_002905 ·
450 aa · 3065441–3066793 (+) ·
RefSeq NP_217247.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | hypothetical protein |
|---|---|
| MTBC0 PGAP re-annotation | DUF349 domain-containing protein |
| Revised (this work) | Conserved hypothetical protein; DUF domain(s) DUF349. Function unknown. |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
I6XF60
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Conserved alanine and arginine rich protein |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
D Cell cycle control, cell division, chromosome partitioning
|
|---|---|
| eggNOG description | Domain of Unknown Function (DUF349) |
| Orthologous group | COG1196 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.45 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 3 synonymous, 4 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
DUF349 | PF03993.18 | 1.5e-21 | 217–289 | Domain of Unknown Function (DUF349) |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: rbpA (RNA polymerase-binding protein RbpA), high confidence from genomic context alone (score 753 excluding text-mining). This association is the citable seed of a function hypothesis for this hypothetical protein.
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1709 scpA |
segregation and condensation protein ScpA | 820 | 801 | experimental:788 |
Rv2730 hyp |
hypothetical protein | 783 | 784 ctx | neighborhood:781 |
Rv2050 rbpA |
RNA polymerase-binding protein RbpA | 752 | 753 ctx | cooccurence:750 |
Rv2699c hyp |
hypothetical protein | 739 | 739 ctx | cooccurence:738 |
Rv2413c hyp |
hypothetical protein | 733 | 722 ctx | cooccurence:715 |
Rv2219 |
transmembrane protein | 693 | 694 ctx | cooccurence:692 |
Rv2708c hyp |
hypothetical protein | 687 | 688 ctx | cooccurence:686 |
Rv1830 |
HTH-type transcriptional regulator | 664 | 663 ctx | cooccurence:659 |
Rv2729c |
integral membrane protein | 645 | 631 ctx | neighborhood:570 |
Rv1390 rpoZ |
DNA-directed RNA polymerase subunit omega | 631 | 631 ctx | cooccurence:627 |
Rv2696c hyp |
hypothetical protein | 622 | 622 ctx | cooccurence:620 |
Rv1423 whiA |
transcriptional regulator WhiA | 619 | 620 ctx | cooccurence:611 |
Rv0005 gyrB |
DNA gyrase subunit B | 652 | 615 | |
Rv2680 hyp |
hypothetical protein | 599 | 600 ctx | cooccurence:597 |
Rv2727c miaA |
tRNA delta(2)-isopentenylpyrophosphate transferase | 568 | 568 ctx | neighborhood:506 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: hypothetical protein
- MTBC0 PGAP product: DUF349 domain-containing protein
- Pfam (hmmscan --cut_ga): DUF349 PF03993.18 (E=1e-21)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_217247.1)
- Domains: Pfam-A via hmmscan --cut_ga — DUF349 (PF03993.18)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1196 - Curated reference: UniProt I6XF60 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
57 functional partner(s); context anchor
rbpA - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_002905|Rv2731| MTADEPRSDDSSGSAPQPAATPVPRPGPRPGPRPVPRPTSYPVGAHPPSDPHRFGRIDDDGTVWLVSASGERIVGSWQAGDPEAAFAHFGRRFDDLSTEIMLMDERLASGTGDARKIKAHAIALAETLPTACVLGDVDALADRLTSIRDRAEVIAAADRSRREEHRAAQTARKEALAAEAEELAANATQWKVAGDRLRAILDEWKTISGVDRKVDDALWKRYSTARDTFNRRRGSHFAELDRERSGVRQSKERLCERAEELSESTDWTATSAEFRKLLADWKAAGRASKDVDDALWRRFKAAQDSFFTARNAATAEKEAELRANADAKEALLAEAERLDTTNHEAARAALRSIAEKWDAIGKVSRERAAELERRLRAVEKKVREAGEADWSDPQARARAEQFRARAEQFEHQAEKAAAAGRTKEADEAKANAEQWRQWAEAAADALTRRP