Rv2739c Resolved · high auto-curated
H37Rv Rv2739c · MTBC0 mtbc0_002914 ·
388 aa · 3074438–3075604 (-) ·
RefSeq NP_217255.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | transferase |
|---|---|
| MTBC0 PGAP re-annotation | glycosyltransferase |
| Revised (this work) | Glycosyltransferase. Pfam: Glyco_tran_28_C (PF04101.23), EryCIII-like_C (PF06722.19), UDPGT (PF00201.25). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
O33282
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Possible alanine rich transferase |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
C Energy production and conversionG Carbohydrate transport and metabolism
|
|---|---|
| eggNOG description | glycosyl transferase |
| Orthologous group | COG1819 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.82 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 5 synonymous, 10 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Glyco_tran_28_C | PF04101.23 | 8.1e-08 | 285–369 | Glycosyltransferase family 28 C-terminal domain |
EryCIII-like_C | PF06722.19 | 1.9e-09 | 286–385 | Erythromycin biosynthesis protein CIII-like, C-terminal domain |
UDPGT | PF00201.25 | 6.1e-06 | 295–363 | UDP-glucoronosyl and UDP-glucosyl transferase |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: ephG (epoxide hydrolase), high confidence from genomic context alone (score 789 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2738c hyp |
hypothetical protein | 884 | 885 ctx | neighborhood:879 |
Rv2740 ephG |
epoxide hydrolase | 789 | 789 ctx | neighborhood:788 |
Rv3909 hyp |
hypothetical protein | 750 | 751 ctx | cooccurence:747 |
Rv3835 hyp |
hypothetical protein | 688 | 689 ctx | cooccurence:683 |
Rv2843 hyp |
hypothetical protein | 664 | 665 ctx | cooccurence:661 |
Rv3755c hyp |
hypothetical protein | 643 | 644 ctx | cooccurence:642 |
Rv0206c mmpL3 |
transmembrane transport protein MmpL3 | 640 | 640 ctx | cooccurence:638 |
Rv2001 hyp |
hypothetical protein | 634 | 634 ctx | cooccurence:634 |
Rv1111c hyp |
hypothetical protein | 572 | 572 ctx | cooccurence:559 |
Rv0466 hyp |
hypothetical protein | 545 | 545 ctx | cooccurence:541 |
Rv2709 |
transmembrane protein | 543 | 543 ctx | cooccurence:543 |
Rv2736c recX |
regulatory protein RecX | 536 | 537 ctx | neighborhood:532 |
Rv2737c recA |
recombinase A | 522 | 522 ctx | neighborhood:519 |
Rv1275 lprC |
lipoprotein LprC | 521 | 522 ctx | cooccurence:520 |
Rv1752 hyp |
hypothetical protein | 519 | 520 ctx | cooccurence:510 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: transferase
- MTBC0 PGAP product: glycosyltransferase
- Pfam (hmmscan --cut_ga): Glyco_tran_28_C PF04101.23 (E=8e-08), EryCIII-like_C PF06722.19 (E=2e-09), UDPGT PF00201.25 (E=6e-06)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_217255.1)
- Domains: Pfam-A via hmmscan --cut_ga — Glyco_tran_28_C (PF04101.23), EryCIII-like_C (PF06722.19), UDPGT (PF00201.25)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1819 - Curated reference: UniProt O33282 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
56 functional partner(s); context anchor
ephG - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_002914|Rv2739c| MRVAVVAGPDPGHSFPAIALCQRFRAAADTPTLFTGVEWLEAARAAGIDAVELDGLAATDRDLDAGARIHRRAAQMAVLNVPRLRALEPELVVSDVITACGGMAAELLGIPWVELNPHPLYLPSKGLPPIGSGLAAGTGIRGRLRDATMRALTGRSWRAGLRQRAAVRVEIGLPARDPGPLRRLIATLPALEVPRPDWPAEAVVVGPLHFEPTDRVLAIPAGTGPVVVVAPSTALTGTAGLTEVALQSLTPGETVPSGSRLVVSRLSGADLTVPPWAVAGLGSQAELLTRADLVICGGGHGMVAKTLLAGVPMVVVPGGGDQWEIANRVVRQGSAVLIRPLTADALVAAVNEVLSSPRFREAARRAAASVAGAADPVRVCHDALALAG