Rv2723 Family assigned · medium auto-curated
H37Rv Rv2723 · MTBC0 mtbc0_002897 ·
397 aa · 3057324–3058517 (+) ·
RefSeq NP_217239.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | integral membrane protein |
|---|---|
| MTBC0 PGAP re-annotation | TerC/Alx family metal homeostasis membrane protein |
| Revised (this work) | TerC/Alx family metal homeostasis membrane protein. Pfam: TerC (PF03741.23). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WG93
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Uncharacterized membrane protein Rv2723 |
UniProt still lists this protein as Uncharacterized membrane protein Rv2723; the revised annotation above is ahead of the current UniProt record.
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
P Inorganic ion transport and metabolism
|
|---|---|
| eggNOG description | membrane protein TerC |
| Orthologous group | COG0861 |
| KEGG orthology |
K05794
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | n/a |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 0 synonymous, 10 missense, 1 nonsense, 0 frameshift |
| Disruption | 1 distinct premature-stop/frameshift site(s); most common in 0.16% of strains (231) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
TerC | PF03741.23 | 8.3e-44 | 69–276 | Integral membrane protein TerC family |
Functional interaction network (STRING v12, guilt-by-association)
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2722 hyp |
hypothetical protein | 746 | 746 ctx | neighborhood:744 |
Rv2721c hyp |
hypothetical protein | 554 | 554 ctx | neighborhood:551 |
Rv1562c treZ |
malto-oligosyltrehalose trehalohydrolase | 655 | 52 | textmining:652 |
Rv3571 kshB |
3-ketosteroid-9-alpha-hydroxylase reductase subunit | 631 | 46 | textmining:630 |
Rv0925c hyp |
hypothetical protein | 870 | 44 | textmining:870 |
Rv3138 pflA |
pyruvate formate lyase activating protein PflA | 828 | 44 | textmining:828 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: integral membrane protein
- MTBC0 PGAP product: TerC/Alx family metal homeostasis membrane protein
- Pfam (hmmscan --cut_ga): TerC PF03741.23 (E=8e-44)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_217239.1)
- Domains: Pfam-A via hmmscan --cut_ga — TerC (PF03741.23)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0861 - Curated reference: UniProt P9WG93 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023, doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 — 6 functional partner(s)
- Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_002897|Rv2723| MGASGLVWTLTIVLIAGLMLVDYVLHVRKTHVPTLRQAVIQSATFVGIAILFGIAVVVFGGSELAVEYFACYLTDEALSVDNLFVFLVIISSFGVPRLAQQKVLLFGIAFALVTRTGFIFVGAALIENFNSAFYLFGLVLLVMAGNLARPTGLESRDAETLKRSVIIRLADRFLRTSQDYNGDRLFTVSNNKRMMTPLLLVMIAVGGTDILFAFDSIPALFGLTQNVYLVFAATAFSLLGLRQLYFLIDGLLDRLVYLSYGLAVILGFIGVKLMLEALHDNKIPFINGGKPVPTVEVSTTQSLTVIIIVLLITTAASFWSARGRAQNAMARARRYATAYLDLHYETESAERDKIFTALLAAERQINTLPTKYRMQPGQDDDLMTLLCRAHAARDAHM