moaA1 Resolved · high auto-curated
H37Rv Rv3109 · MTBC0 - ·
359 aa · 3477649–3478728 (+) ·
RefSeq YP_177925.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | cyclic pyranopterin monophosphate synthase |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | Cyclic pyranopterin monophosphate synthase. Pfam: Radical_SAM (PF04055.28), Mob_synth_C (PF06463.19). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
P9WJS3
SwissProt · reviewed
· Evidence at transcript level
|
|---|---|
| UniProt name | GTP 3',8-cyclase 1 |
| EC (curated) |
EC 4.1.99.22
|
| Curated function | Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
H Coenzyme transport and metabolism
|
|---|---|
| Preferred name | moaA |
| eggNOG description | Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8- dihydroguanosine 5'-triphosphate |
| Orthologous group | COG2896 |
| EC number |
EC 4.1.99.22
|
| KEGG orthology |
K03639
|
| KEGG pathways |
map00790, map01100, map04122
|
| Gene Ontology (8) |
GO:0005575, GO:0005623, GO:0005886, GO:0008150, GO:0016020, GO:0040007, GO:0044464, GO:0071944
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.645 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 4 synonymous, 8 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Radical_SAM | PF04055.28 | 1.0e-33 | 32–197 | Radical SAM superfamily |
Mob_synth_C | PF06463.19 | 1.0e-34 | 203–326 | Molybdenum Cofactor Synthesis C |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: moaC1 (cyclic pyranopterin monophosphate synthase accessory protein), high confidence from genomic context alone (score 1000 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3111 moaC1 |
cyclic pyranopterin monophosphate synthase accessory protein | 999 | 1000 ctx | neighborhood:634 fusion:592 cooccurence:772 coexpression:948 database:900 textmining:863 |
Rv0864 moaC2 |
cyclic pyranopterin monophosphate synthase accessory protein | 999 | 999 ctx | fusion:900 cooccurence:772 coexpression:647 database:900 textmining:901 |
Rv3324c moaC3 |
cyclic pyranopterin monophosphate synthase accessory protein | 999 | 996 ctx | fusion:579 cooccurence:772 coexpression:649 database:900 textmining:901 |
Rv3112 moaD1 |
molybdenum cofactor biosynthesis protein MoaD | 991 | 958 ctx | neighborhood:592 coexpression:901 textmining:815 |
Rv3110 moaB1 |
pterin-4-alpha-carbinolamine dehydratase | 988 | 944 ctx | neighborhood:613 coexpression:860 textmining:806 |
Rv0869c moaA2 |
molybdenum cofactor biosynthesis protein MoaA | 933 | 918 | database:900 |
Rv3113 |
phosphatase | 913 | 914 ctx | neighborhood:408 coexpression:860 |
Rv3119 moaE1 |
molybdopterin synthase catalytic subunit 1 | 970 | 913 ctx | cooccurence:667 coexpression:402 database:500 textmining:671 |
Rv3114 hyp |
hypothetical protein | 913 | 913 | coexpression:868 |
Rv0866 moaE2 |
molybdopterin synthase catalytic subunit 2 | 991 | 910 ctx | cooccurence:688 coexpression:402 database:500 textmining:909 |
Rv1940 ribA1 |
riboflavin biosynthesis protein RibA | 909 | 906 | database:900 |
Rv3609c folE |
GTP cyclohydrolase I | 906 | 906 | database:900 |
Rv1415 ribA2 |
bifunctional riboflavin biosynthesis GTP cyclohydrolase II/3,4-dihydroxy-2-butanone 4-phosphate synthase | 907 | 904 | database:900 |
Rv3323c moaX |
MoaD-MoaE fusion protein MoaX | 969 | 895 ctx | cooccurence:677 coexpression:648 textmining:720 |
Rv0438c moeA2 |
molybdopterin molybdenumtransferase | 853 | 835 ctx | cooccurence:766 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): cyclic pyranopterin monophosphate synthase
- Pfam (hmmscan --cut_ga): Radical_SAM PF04055.28 (E=1e-33), Mob_synth_C PF06463.19 (E=1e-34)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq YP_177925.1)
- Domains: Pfam-A via hmmscan --cut_ga — Radical_SAM (PF04055.28), Mob_synth_C (PF06463.19)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG2896 - Curated reference: UniProt P9WJS3 (SwissProt, reviewed; Evidence at transcript level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
50 functional partner(s); context anchor
moaC1 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv3109|moaA1 MSTPTLPDMVAPSPRVRVKDRCRRMMGDLRLSVIDQCNLRCRYCMPEEHYTWLPRQDLLSVKEISAIVDVFLSVGVSKVRITGGEPLIRPDLPEIVRTLSAKVGEDSGLRDLAITTNGVLLADRVDGLKAAGMKRITVSLDTLQPERFKAISQRNSHDKVIAGIKAVAAAGFTDTKIDTTVMRGANHDELADLIEFARTVNAEVRFIEYMDVGGATHWAWEKVFTKANMLESLEKRYGRIEPLPKHDTAPANRYALPDGTTFGIIASTTEPFCATCDRSRLTADGLWLHCLYAISGINLREPLRAGATHDDLVETVTTGWRRRTDRGAEQRLAQRERGVFLPLSTLKADPHLEMHTRGG