moaB1 Resolved · high auto-curated
H37Rv Rv3110 · MTBC0 - ·
131 aa · 3478779–3479174 (+) ·
RefSeq YP_177926.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | pterin-4-alpha-carbinolamine dehydratase |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | Pterin-4-alpha-carbinolamine dehydratase. Pfam: Pterin_4a (PF01329.25). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
Q6MX13
TrEMBL · unreviewed
· Inferred from homology
|
|---|---|
| UniProt name | Putative pterin-4-alpha-carbinolamine dehydratase |
| EC (curated) |
EC 4.2.1.96
|
UniProt still lists this protein as Putative pterin-4-alpha-carbinolamine dehydratase; the revised annotation above is ahead of the current UniProt record.
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
H Coenzyme transport and metabolism
|
|---|---|
| Preferred name | moaB1 |
| eggNOG description | pterin-4-alpha-carbinolamine dehydratase |
| Orthologous group | COG2154 |
| EC number |
EC 4.2.1.96
|
| KEGG orthology |
K01724
|
| KEGG pathways |
map00790
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Pterin_4a | PF01329.25 | 4.5e-23 | 31–119 | Pterin 4 alpha carbinolamine dehydratase |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: moaC1 (cyclic pyranopterin monophosphate synthase accessory protein), high confidence from genomic context alone (score 972 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3111 moaC1 |
cyclic pyranopterin monophosphate synthase accessory protein | 989 | 972 ctx | neighborhood:801 coexpression:863 textmining:652 |
Rv3112 moaD1 |
molybdenum cofactor biosynthesis protein MoaD | 992 | 961 ctx | neighborhood:736 coexpression:860 textmining:807 |
Rv3109 moaA1 |
cyclic pyranopterin monophosphate synthase | 988 | 944 ctx | neighborhood:613 coexpression:860 textmining:806 |
Rv3113 |
phosphatase | 940 | 938 ctx | neighborhood:489 coexpression:866 |
Rv3114 hyp |
hypothetical protein | 920 | 920 ctx | neighborhood:453 coexpression:860 |
Rv1159A |
4a-hydroxytetrahydrobiopterin dehydratase | 918 | 918 | database:900 |
Rv3124 moaR1 |
transcriptional regulator MoaR | 904 | 734 | coexpression:734 textmining:654 |
Rv0976c hyp |
hypothetical protein | 482 | 433 | coexpression:413 |
Rv0193c hyp |
hypothetical protein | 408 | 409 | coexpression:409 |
Rv3108 hyp |
hypothetical protein | 445 | 403 | |
Rv0059 darT hyp |
hypothetical protein | 424 | 399 | |
Rv3116 moeB2 |
molybdenum cofactor biosynthesis protein MoeB | 734 | 259 | textmining:657 |
Rv3119 moaE1 |
molybdopterin synthase catalytic subunit 1 | 719 | 220 | textmining:655 |
Rv1177 fdxC |
ferredoxin FdxC | 474 | 150 | textmining:407 |
Rv2238c ahpE |
peroxiredoxin | 449 | 99 | textmining:414 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): pterin-4-alpha-carbinolamine dehydratase
- Pfam (hmmscan --cut_ga): Pterin_4a PF01329.25 (E=4e-23)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq YP_177926.1)
- Domains: Pfam-A via hmmscan --cut_ga — Pterin_4a (PF01329.25)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG2154 - Curated reference: UniProt Q6MX13 (TrEMBL, unreviewed; Inferred from homology)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
25 functional partner(s); context anchor
moaC1 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv3110|moaB1 MTVSTPEQHEQRASHDASEGKHNVCQGRLAALADAAVSEKLGALPGWQLLDMRLSRAFQCTNFDQSIDFMNRVASIANDINHHPDIAVLDKRSVRVTAWTRKLGYLTDIDFDLAASVEAMYATEFADRPAR