moaD1 Resolved · high auto-curated
H37Rv Rv3112 · MTBC0 - ·
83 aa · 3479700–3479951 (+) ·
RefSeq YP_177928.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | molybdenum cofactor biosynthesis protein MoaD |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | Molybdenum cofactor biosynthesis protein MoaD. Pfam: ThiS (PF02597.27). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
L7N6B4
SwissProt · reviewed
· Inferred from homology
|
|---|---|
| UniProt name | Molybdopterin synthase sulfur carrier subunit |
| Curated function | Involved in sulfur transfer in the conversion of molybdopterin precursor Z to molybdopterin (By similarity). Probably plays a role in host phagosome maturation arrest. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
H Coenzyme transport and metabolism
|
|---|---|
| Preferred name | moaD |
| eggNOG description | Mo-molybdopterin cofactor metabolic process |
| Orthologous group | COG1977 |
| EC number |
EC 2.8.1.12
|
| KEGG orthology |
K03636, K21142
|
| KEGG pathways |
map00790, map01100, map04122
|
| Gene Ontology (40) |
GO:0005575, GO:0005622, GO:0005623, GO:0005737, GO:0005829, GO:0006732, GO:0006777, GO:0006793, GO:0006796, GO:0006807, GO:0008150, GO:0008152 +28 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.0 · strong purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 1 synonymous, 0 missense, 0 nonsense, 1 frameshift |
| Disruption | 1 distinct premature-stop/frameshift site(s); most common in 0.12% of strains (181) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
ThiS | PF02597.27 | 1.2e-17 | 6–83 | ThiS family |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: moaC1 (cyclic pyranopterin monophosphate synthase accessory protein), high confidence from genomic context alone (score 980 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3119 moaE1 |
molybdopterin synthase catalytic subunit 1 | 997 | 981 | coexpression:493 experimental:463 database:900 textmining:888 |
Rv3111 moaC1 |
cyclic pyranopterin monophosphate synthase accessory protein | 996 | 980 ctx | neighborhood:736 coexpression:928 textmining:814 |
Rv0866 moaE2 |
molybdopterin synthase catalytic subunit 2 | 976 | 975 | coexpression:491 experimental:463 database:900 |
Rv3323c moaX |
MoaD-MoaE fusion protein MoaX | 974 | 972 | coexpression:494 experimental:463 database:900 |
Rv3110 moaB1 |
pterin-4-alpha-carbinolamine dehydratase | 992 | 961 ctx | neighborhood:736 coexpression:860 textmining:807 |
Rv3109 moaA1 |
cyclic pyranopterin monophosphate synthase | 991 | 958 ctx | neighborhood:592 coexpression:901 textmining:815 |
Rv3116 moeB2 |
molybdenum cofactor biosynthesis protein MoeB | 986 | 957 | experimental:415 database:900 textmining:693 |
Rv3206c moeB1 |
adenylyltransferase/sulfurtransferase MoeZ | 989 | 945 | experimental:415 database:900 textmining:827 |
Rv3113 |
phosphatase | 931 | 932 ctx | neighborhood:535 coexpression:859 |
Rv3114 hyp |
hypothetical protein | 925 | 925 ctx | neighborhood:489 coexpression:860 |
Rv1335 cysO |
sulfur carrier protein CysO | 944 | 921 | database:900 |
Rv0868c moaD2 |
cyclic pyranopterin monophosphate synthase | 979 | 900 | database:900 textmining:803 |
Rv3124 moaR1 |
transcriptional regulator MoaR | 851 | 615 | coexpression:615 textmining:630 |
Rv2735c hyp |
hypothetical protein | 555 | 555 ctx | cooccurence:555 |
Rv2337c hyp |
hypothetical protein | 552 | 531 | experimental:415 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): molybdenum cofactor biosynthesis protein MoaD
- Pfam (hmmscan --cut_ga): ThiS PF02597.27 (E=1e-17)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq YP_177928.1)
- Domains: Pfam-A via hmmscan --cut_ga — ThiS (PF02597.27)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1977 - Curated reference: UniProt L7N6B4 (SwissProt, reviewed; Inferred from homology)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
45 functional partner(s); context anchor
moaC1 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv3112|moaD1 MIKVNVLYFGAVREACDETPREEVEVQNGTDVGNLVDQLQQKYPRLRDHCQRVQMAVNQFIAPLSTVLGDGDEVAFIPQVAGG