lipY Resolved · high auto-curated
H37Rv Rv3097c · MTBC0 - ·
437 aa · 3465778–3467091 (-) ·
RefSeq YP_177924.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | triacylglycerol lipase Lip |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | Triacylglycerol lipase Lip. Pfam: PE (PF00934.26), Abhydrolase_3 (PF07859.20), Say1_Mug180 (PF10340.16). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
I6Y2J4
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Triacylglycerol lipase |
| EC (curated) |
EC 3.1.1.3
|
| Curated function | Catalyzes the hydrolysis of both intracellular and extracellular triacylglycerol (TAG). In vitro, can also hydrolyze p-nitrophenyl (pNP) esters with various chain lengths, including pNP-acetate (C2), pNP-butyrate (C4), pNP-caproate (C6), pNP-caprylate (C8), pNP-laurate (C12), pNP-myristate (C14), pNP-palmitate (C16) and pNP-stearate (C18). Also hydrolyzes monobutyrin, tributyrin and trioctanoin. Overexpression results in increase of virulence characterized by reduced survival of infected mouse and increased burden of bacilli in the lungs. Hydrolyzes internal or host-derived TAG depending on it. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
I Lipid transport and metabolism
|
|---|---|
| eggNOG description | Steryl acetyl hydrolase |
| Orthologous group | COG0657 |
| EC number |
EC 3.1.1.3
|
| KEGG orthology |
K01046
|
| KEGG pathways |
map00561, map01100
|
| KEGG modules |
M00098
|
| Gene Ontology (7) |
GO:0003674, GO:0003824, GO:0004806, GO:0016298, GO:0016787, GO:0016788, GO:0052689
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.699 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 5 synonymous, 10 missense, 0 nonsense, 2 frameshift |
| Disruption | 2 distinct premature-stop/frameshift site(s); most common in 0.85% of strains (1237) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
PE | PF00934.26 | 5.4e-31 | 5–95 | PE family |
Abhydrolase_3 | PF07859.20 | 2.8e-19 | 235–416 | alpha/beta hydrolase fold |
Say1_Mug180 | PF10340.16 | 6.8e-08 | 235–343 | Steryl acetyl hydrolase |
Functional interaction network (STRING v12, guilt-by-association)
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3088 tgs4 |
diacyglycerol O-acyltransferase | 927 | 925 | database:900 |
Rv3480c |
diacyglycerol O-acyltransferase | 926 | 925 | database:900 |
Rv3087 |
diacyglycerol O-acyltransferase | 923 | 923 | database:900 |
Rv2285 |
diacylglycerol acyltransferase | 923 | 923 | database:900 |
Rv0895 |
diacyglycerol O-acyltransferase | 921 | 922 | database:900 |
Rv3740c |
diacyglycerol O-acyltransferase | 919 | 919 | database:900 |
Rv3734c tgs2 |
diacyglycerol O-acyltransferase | 919 | 917 | database:900 |
Rv1425 |
diacyglycerol O-acyltransferase | 915 | 915 | database:900 |
Rv1760 |
diacylglycerol acyltransferase | 914 | 915 | database:900 |
Rv3371 |
diacyglycerol O-acyltransferase | 912 | 913 | database:900 |
Rv2252 dagK |
diacylglycerol kinase | 906 | 907 | database:900 |
Rv2484c |
diacyglycerol O-acyltransferase | 904 | 904 | database:900 |
Rv3130c tgs1 |
diacyglycerol O-acyltransferase | 925 | 901 | database:900 |
Rv3804c fbpA |
diacylglycerol acyltransferase/mycolyltransferase Ag85A | 904 | 901 | database:900 |
Rv0129c fbpC |
diacylglycerol acyltransferase/mycolyltransferase Ag85C | 904 | 901 | database:900 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): triacylglycerol lipase Lip
- Pfam (hmmscan --cut_ga): PE PF00934.26 (E=5e-31), Abhydrolase_3 PF07859.20 (E=3e-19), Say1_Mug180 PF10340.16 (E=7e-08)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq YP_177924.1)
- Domains: Pfam-A via hmmscan --cut_ga — PE (PF00934.26), Abhydrolase_3 (PF07859.20), Say1_Mug180 (PF10340.16)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0657 - Curated reference: UniProt I6Y2J4 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023, doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 — 71 functional partner(s)
- Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv3097c|lipY MVSYVVALPEVMSAAATDVASIGSVVATASQGVAGATTTVLAAAEDEVSAAIAALFSGHGQDYQALSAQLAVFHERFVQALTGAAKGYAAAELANASLLQSEFASGIGNGFATIHQEIQRAPTALAAGFTQVPPFAAAQAGIFTGTPSGAAGFDIASLWPVKPLLSLSALETHFAIPNNPLLALIASDIPPLSWFLGNSPPPLLNSLLGQTVQYTTYDGMSVVQITPAHPTGEYVVAIHGGAFILPPSIFHWLNYSVTAYQTGATVQVPIYPLVQEGGTAGTVVPAMAGLISTQIAQHGVSNVSVVGDSAGGNLALAAAQYMVSQGNPVPSSMVLLSPWLDVGTWQISQAWAGNLAVNDPLVSPLYGSLNGLPPTYVYSGSLDPLAQQAVVLEHTAVVQGAPFSFVLAPWQIHDWILLTPWGLLSWPQINQQLGIAA