ftsK Resolved · high auto-curated
H37Rv Rv2748c · MTBC0 mtbc0_002925 ·
883 aa · 3082270–3084921 (-) ·
RefSeq NP_217264.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | DNA translocase FtsK |
|---|---|
| MTBC0 PGAP re-annotation | DNA translocase FtsK |
| Revised (this work) | DNA translocase FtsK. Pfam: FtsK_4TM (PF13491.12), FtsK_alpha (PF17854.7), FtsK_SpoIIIE (PF01580.25), FtsK_gamma (PF09397.17). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WNA3
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | DNA translocase FtsK |
| Curated function | Essential cell division protein that coordinates cell division and chromosome segregation. The N-terminus is involved in assembly of the cell-division machinery. The C-terminus functions as a DNA motor that moves dsDNA in an ATP-dependent manner towards the dif recombination site, which is located within the replication terminus region. Required for activation of the Xer recombinase, allowing activation of chromosome unlinking by recombination (By similarity). |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
D Cell cycle control, cell division, chromosome partitioning
|
|---|---|
| Preferred name | ftsK |
| eggNOG description | FtsK SpoIIIE |
| Orthologous group | COG1674 |
| KEGG orthology |
K03466
|
| Gene Ontology (14) |
GO:0005575, GO:0005576, GO:0005618, GO:0005622, GO:0005623, GO:0005737, GO:0005829, GO:0008150, GO:0030312, GO:0040007, GO:0044424, GO:0044444 +2 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.435 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 5 synonymous, 6 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
FtsK_4TM | PF13491.12 | 6.8e-06 | 173–291 | 4TM region of DNA translocase FtsK/SpoIIIE |
FtsK_alpha | PF17854.7 | 4.2e-27 | 396–494 | FtsK alpha domain |
FtsK_SpoIIIE | PF01580.25 | 3.4e-74 | 502–702 | FtsK/SpoIIIE family |
FtsK_gamma | PF09397.17 | 6.5e-23 | 808–865 | Ftsk gamma domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: parB (chromosome partitioning protein ParB), high confidence from genomic context alone (score 762 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3917c parB |
chromosome partitioning protein ParB | 880 | 762 ctx | cooccurence:757 textmining:517 |
Rv3918c parA |
chromosome partitioning protein ParA | 665 | 649 ctx | cooccurence:637 |
Rv2751 hyp |
hypothetical protein | 615 | 614 ctx | neighborhood:609 |
Rv1708 |
initiation inhibitor protein | 678 | 613 ctx | cooccurence:597 |
Rv2163c pbpB |
penicillin-binding membrane protein PbpB | 889 | 612 ctx | cooccurence:535 textmining:727 |
Rv2894c xerC |
tyrosine recombinase XerC | 756 | 604 ctx | cooccurence:578 textmining:409 |
Rv1701 xerD |
tyrosine recombinase XerD | 717 | 602 ctx | cooccurence:596 |
Rv0639 nusG |
transcription termination/antitermination protein NusG | 599 | 600 ctx | cooccurence:579 |
Rv2166c mraZ |
transcriptional regulator MraZ | 623 | 599 ctx | cooccurence:568 |
Rv2749 hyp |
hypothetical protein | 599 | 599 ctx | neighborhood:587 |
Rv3213c |
SOJ/ParA-like protein | 615 | 597 ctx | cooccurence:580 |
Rv2750 |
dehydrogenase | 592 | 592 ctx | neighborhood:586 |
Rv0668 rpoC |
DNA-directed RNA polymerase subunit beta' | 612 | 589 ctx | cooccurence:572 |
Rv2156c murX |
phospho-N-acetylmuramoyl-pentappeptidetransferase | 577 | 577 ctx | cooccurence:564 |
Rv2154c ftsW |
lipid II flippase FtsW | 833 | 560 ctx | cooccurence:494 textmining:637 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: DNA translocase FtsK
- MTBC0 PGAP product: DNA translocase FtsK
- Pfam (hmmscan --cut_ga): FtsK_4TM PF13491.12 (E=7e-06), FtsK_alpha PF17854.7 (E=4e-27), FtsK_SpoIIIE PF01580.25 (E=3e-74), FtsK_gamma PF09397.17 (E=6e-23)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_217264.1)
- Domains: Pfam-A via hmmscan --cut_ga — FtsK_4TM (PF13491.12), FtsK_alpha (PF17854.7), FtsK_SpoIIIE (PF01580.25), FtsK_gamma (PF09397.17)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1674 - Curated reference: UniProt P9WNA3 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
88 functional partner(s); context anchor
parB - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_002925|Rv2748c|ftsK MLGPPGTPRVGRRDAARSLVTLLRRPWQRGEQIAVTSVADGVDGVIATRLAVMSSKTVARSGTRTSRSKATSRGASRSARSAVPRKRSRPVKGVGRPSRRHHRSLLVSTGLACGRAMRAVWMMAAKGTGGAARSIGRARDIEPGHRRDGIALVLLGLAVVVAASSWFDAARPLGAWVDALLRTFIGSAVVMLPLVAAAVAVVLMRTSPNPDSRPRLILGASLIGLSFLGLCHLWAGSPEAPESRLRAAGFIGFAIGGPLSDGLTAWIAAPLLFIGALFGLLLLAGITIREVPDAMRAMFGTRLLPREYADDFEDFADFDGDDADTVEVARQDFSDGYYDEVPLCSDDGPPAWPSAEVPQDDTATIPEASAGRGSGRRGRRKDTQVLDRIVEGPYTLPSLDLLISGDPPKKRSAANTHMAGAIGEVLTQFKVDAAVTGCTRGPTVTRYEVELGPGVKVEKITALQRNIAYAVATESVRMLAPIPGKSAVGIEVPNTDREMVRLADVLTARETRRDHHPLVIGLGKDIEGDFISANLAKMPHLLVAGSTGSGKSSFVNSMLVSLLTRATPEEVRMILIDPKMVELTPYEGIPHLITPIITQPKKAAAALAWLVDEMEQRYQDMQASRVRHIDDFNDKVRSGAITAPLGSQREYRPYPYVVAIVDELADLMMTAPRDVEDAIVRITQKARAAGIHLVLATQRPSVDVVTGLIKTNVPSRLAFATSSLTDSRVILDQAGAEKLIGMGDGLFLPMGASKPLRLQGAYVSDEEIHAVVTACKEQAEPEYTEGVTTAKPTAERTDVDPDIGDDMDVFLQAVELVVSSQFGSTSMLQRKLRVGFAKAGRLMDLMETRGIVGPSEGSKAREVLVKPDELAGTLAAIRGDGGE