argA Resolved · high auto-curated
H37Rv Rv2747 · MTBC0 mtbc0_002924 ·
174 aa · 3081677–3082201 (+) ·
RefSeq NP_217263.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | L-glutamate alpha-N-acetyltranferase |
|---|---|
| MTBC0 PGAP re-annotation | amino-acid N-acetyltransferase |
| Revised (this work) | Amino-acid N-acetyltransferase. Pfam: Acetyltransf_1 (PF00583.32), Acetyltransf_7 (PF13508.14). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
O33289
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Amino-acid acetyltransferase |
| EC (curated) |
EC 2.3.1.1
|
| Curated function | Catalyzes the conversion of L-glutamate to alpha-N-acetyl-L-glutamate. L-glutamine is a significantly better substrate compared to L-glutamate. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
E Amino acid transport and metabolism
|
|---|---|
| Preferred name | argA |
| eggNOG description | N-acetylglutamate synthase |
| Orthologous group | COG1246 |
| EC number |
EC 2.3.1.1
|
| KEGG orthology |
K00619
|
| KEGG pathways |
map00220, map01100, map01110, map01130, map01210, map01230
|
| KEGG modules |
M00028
|
| Gene Ontology (54) |
GO:0003674, GO:0003824, GO:0004042, GO:0005488, GO:0005515, GO:0006082, GO:0006520, GO:0006525, GO:0006526, GO:0006591, GO:0006807, GO:0008080 +42 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.0 · strong purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 1 synonymous, 0 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Acetyltransf_1 | PF00583.32 | 5.7e-13 | 49–127 | Acetyltransferase (GNAT) family |
Acetyltransf_7 | PF13508.14 | 5.5e-12 | 49–129 | Acetyltransferase (GNAT) domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: pgsA3 (CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase), high confidence from genomic context alone (score 721 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1654 argB |
acetylglutamate kinase | 960 | 939 | database:900 |
Rv1653 argJ |
bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase | 989 | 937 | database:900 textmining:845 |
Rv0428c |
GCN5-like N-acetyltransferase | 907 | 903 | database:900 |
Rv0337c aspC |
aspartate aminotransferase | 906 | 901 | database:900 |
Rv2476c gdh |
NAD-dependent glutamate dehydrogenase | 900 | 900 | database:900 |
Rv2746c pgsA3 |
CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase | 721 | 721 ctx | neighborhood:649 |
Rv3859c gltB |
glutamate synthase large subunit | 734 | 545 ctx | neighborhood:544 textmining:440 |
Rv1658 argG |
argininosuccinate synthase | 629 | 517 | coexpression:477 |
Rv2745c clgR |
transcriptional regulator ClgR | 511 | 511 ctx | neighborhood:503 |
Rv2169c |
transmembrane protein | 489 | 490 ctx | cooccurence:489 |
Rv0637 hadC |
(3R)-hydroxyacyl-ACP dehydratase subunit HadC | 486 | 486 ctx | cooccurence:483 |
Rv1652 argC |
N-acetyl-gamma-glutamyl-phoshate reductase | 659 | 426 | textmining:430 |
Rv0504c hyp |
hypothetical protein | 424 | 424 ctx | cooccurence:420 |
Rv0635 hadA |
(3R)-hydroxyacyl-ACP dehydratase subunit HadA | 415 | 415 ctx | cooccurence:412 |
Rv2146c |
transmembrane protein | 415 | 415 ctx | cooccurence:403 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: L-glutamate alpha-N-acetyltranferase
- MTBC0 PGAP product: amino-acid N-acetyltransferase
- Pfam (hmmscan --cut_ga): Acetyltransf_1 PF00583.32 (E=6e-13), Acetyltransf_7 PF13508.14 (E=6e-12)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_217263.1)
- Domains: Pfam-A via hmmscan --cut_ga — Acetyltransf_1 (PF00583.32), Acetyltransf_7 (PF13508.14)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1246 - Curated reference: UniProt O33289 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
27 functional partner(s); context anchor
pgsA3 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_002924|Rv2747|argA MTERPRDCRPVVRRARTSDVPAIKQLVDTYAGKILLEKNLVTLYEAVQEFWVAEHPDLYGKVVGCGALHVLWSDLGEIRTVAVDPAMTGHGIGHAIVDRLLQVARDLQLQRVFVLTFETEFFARHGFTEIEGTPVTAEVFDEMCRSYDIGVAEFLDLSYVKPNILGNSRMLLVL