wag31 Resolved · high auto-curated
H37Rv Rv2145c · MTBC0 mtbc0_002281 ·
260 aa · 2431995–2432777 (-) ·
RefSeq NP_216661.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | cell wall synthesis protein Wag31 |
|---|---|
| MTBC0 PGAP re-annotation | cell wall synthesis protein Wag31 |
| Revised (this work) | Cell wall synthesis protein Wag31. Pfam: DivIVA (PF05103.19). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WMU1
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Cell wall synthesis protein Wag31 |
| Curated function | Important for maintaining cell shape and cell wall integrity by localizing peptidoglycan synthesis to the cell poles. Protects PbpB (PBP3, FtsI) from oxidative stress-induced cleavage. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
D Cell cycle control, cell division, chromosome partitioning
|
|---|---|
| Preferred name | wag31 |
| eggNOG description | PFAM DivIVA |
| Orthologous group | COG3599 |
| Gene Ontology (28) |
GO:0005575, GO:0005618, GO:0005623, GO:0005886, GO:0008150, GO:0008360, GO:0009273, GO:0009987, GO:0016020, GO:0022603, GO:0022604, GO:0030312 +16 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | n/a |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 0 synonymous, 2 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
DivIVA | PF05103.19 | 8.3e-12 | 3–61 | DivIVA protein |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv2144c (transmembrane protein), high confidence from genomic context alone (score 953 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2144c |
transmembrane protein | 952 | 953 ctx | neighborhood:776 coexpression:798 |
Rv3682 ponA2 |
bifunctional penicillin-insensitive transglycosylase/penicillin-sensitive transpeptidase | 937 | 913 | experimental:848 |
Rv2146c |
transmembrane protein | 899 | 892 ctx | neighborhood:722 coexpression:434 |
Rv2147c sepF |
cell division protein SepF | 954 | 804 ctx | neighborhood:659 coexpression:450 textmining:775 |
Rv0179c lprO |
lipoprotein LprO | 751 | 751 | coexpression:751 |
Rv2721c hyp |
hypothetical protein | 751 | 751 | coexpression:751 |
Rv2148c hyp |
hypothetical protein | 745 | 729 ctx | neighborhood:423 coexpression:549 |
Rv0050 ponA1 |
bifunctional penicillin-insensitive transglycosylase/penicillin-sensitive transpeptidase | 920 | 720 | experimental:708 textmining:729 |
Rv3414c sigD |
ECF RNA polymerase sigma factor SigD | 642 | 643 | coexpression:586 |
Rv3231c hyp |
hypothetical protein | 631 | 631 ctx | cooccurence:628 |
Rv2170 |
GCN5-like N-acetyltransferase | 583 | 583 ctx | cooccurence:582 |
Rv3605c hyp |
hypothetical protein | 566 | 566 ctx | cooccurence:566 |
Rv0014c pknB |
serine/threonine-protein kinase PknB | 980 | 562 | coexpression:413 textmining:957 |
Rv2150c ftsZ |
cell division protein FtsZ | 974 | 545 ctx | neighborhood:421 textmining:947 |
Rv2413c hyp |
hypothetical protein | 543 | 544 ctx | cooccurence:529 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: cell wall synthesis protein Wag31
- MTBC0 PGAP product: cell wall synthesis protein Wag31
- Pfam (hmmscan --cut_ga): DivIVA PF05103.19 (E=8e-12)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216661.1)
- Domains: Pfam-A via hmmscan --cut_ga — DivIVA (PF05103.19)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG3599 - Curated reference: UniProt P9WMU1 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
77 functional partner(s); context anchor
Rv2144c - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_002281|Rv2145c|wag31 MPLTPADVHNVAFSKPPIGKRGYNEDEVDAFLDLVENELTRLIEENSDLRQRINELDQELAAGGGAGVTPQATQAIPAYEPEPGKPAPAAVSAGMNEEQALKAARVLSLAQDTADRLTNTAKAESDKMLADARANAEQILGEARHTADATVAEARQRADAMLADAQSRSEAQLRQAQEKADALQADAERKHSEIMGTINQQRAVLEGRLEQLRTFEREYRTRLKTYLESQLEELGQRGSAAPVDSNADAGGFDQFNRGKN