ftsQ Resolved · high auto-curated
H37Rv Rv2151c · MTBC0 mtbc0_002287 ·
314 aa · 2437076–2438020 (-) ·
RefSeq NP_216667.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | cell division protein FtsQ |
|---|---|
| MTBC0 PGAP re-annotation | cell division protein FtsQ |
| Revised (this work) | Cell division protein FtsQ. Pfam: POTRA_1 (PF08478.16), FtsQ_DivIB_C (PF03799.21). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WNA1
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Cell division protein FtsQ |
| Curated function | Essential cell division protein. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
D Cell cycle control, cell division, chromosome partitioning
|
|---|---|
| Preferred name | ftsQ |
| eggNOG description | cell division protein FtsQ |
| Orthologous group | COG1589 |
| KEGG orthology |
K03589
|
| KEGG pathways |
map04112
|
| Gene Ontology (19) |
GO:0005575, GO:0005622, GO:0005623, GO:0005737, GO:0005829, GO:0005886, GO:0005887, GO:0008150, GO:0016020, GO:0016021, GO:0031224, GO:0031226 +7 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.909 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 2 synonymous, 5 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
POTRA_1 | PF08478.16 | 3.1e-15 | 125–192 | POTRA domain, FtsQ-type |
FtsQ_DivIB_C | PF03799.21 | 7.9e-07 | 205–299 | Cell division protein FtsQ/DivIB, C-terminal |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: murG (UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol-N-acetylglucosamine transferase), high confidence from genomic context alone (score 995 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2153c murG |
UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol-N-acetylglucosamine transferase | 998 | 995 ctx | neighborhood:881 coexpression:962 textmining:746 |
Rv2152c murC |
UDP-N-acetylmuramate--alanine ligase | 997 | 992 ctx | neighborhood:881 coexpression:932 textmining:683 |
Rv2154c ftsW |
lipid II flippase FtsW | 999 | 989 ctx | neighborhood:881 coexpression:843 experimental:443 textmining:958 |
Rv2155c murD |
UDP-N-acetylmuramoylalanine--D-glutamate ligase | 992 | 983 ctx | neighborhood:876 coexpression:865 textmining:608 |
Rv2157c murF |
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase | 984 | 961 ctx | neighborhood:876 coexpression:698 textmining:630 |
Rv1024 |
membrane protein | 983 | 955 | experimental:928 textmining:655 |
Rv2156c murX |
phospho-N-acetylmuramoyl-pentappeptidetransferase | 937 | 928 ctx | neighborhood:876 coexpression:442 |
Rv2158c murE |
UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase | 963 | 926 ctx | neighborhood:876 coexpression:425 textmining:522 |
Rv2164c hyp |
hypothetical protein | 931 | 922 ctx | neighborhood:544 experimental:829 |
Rv2163c pbpB |
penicillin-binding membrane protein PbpB | 994 | 916 ctx | neighborhood:544 experimental:781 textmining:940 |
Rv0732 secY |
preprotein translocase SecY | 902 | 888 | experimental:878 |
Rv2864c |
penicillin-binding lipoprotein | 872 | 858 | experimental:781 |
Rv0016c pbpA |
penicillin-binding protein PbpA | 968 | 840 | experimental:781 textmining:814 |
Rv2150c ftsZ |
cell division protein FtsZ | 991 | 764 ctx | neighborhood:566 coexpression:478 textmining:965 |
Rv2159c hyp |
hypothetical protein | 823 | 750 ctx | neighborhood:700 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: cell division protein FtsQ
- MTBC0 PGAP product: cell division protein FtsQ
- Pfam (hmmscan --cut_ga): POTRA_1 PF08478.16 (E=3e-15), FtsQ_DivIB_C PF03799.21 (E=8e-07)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216667.1)
- Domains: Pfam-A via hmmscan --cut_ga — POTRA_1 (PF08478.16), FtsQ_DivIB_C (PF03799.21)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1589 - Curated reference: UniProt P9WNA1 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
160 functional partner(s); context anchor
murG - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_002287|Rv2151c|ftsQ MTEHNEDPQIERVADDAADEEAVTEPLATESKDEPAEHPEFEGPRRRARRERAERRAAQARATAIEQARRAAKRRARGQIVSEQNPAKPAARGVVRGLKALLATVVLAVVGIGLGLALYFTPAMSAREIVIIGIGAVSREEVLDAARVRPATPLLQIDTQQVADRVATIRRVASARVQRQYPSALRITIVERVPVVVKDFSDGPHLFDRDGVDFATDPPPPALPYFDVDNPGPSDPTTKAALQVLTALHPEVASQVGRIAAPSVASITLTLADGRVVIWGTTDRCEEKAEKLAALLTQPGRTYDVSSPDLPTVK