murG Resolved · high auto-curated
H37Rv Rv2153c · MTBC0 mtbc0_002289 ·
410 aa · 2439498–2440730 (-) ·
RefSeq NP_216669.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol-N-acetylglucosamine transferase |
|---|---|
| MTBC0 PGAP re-annotation | undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase |
| Revised (this work) | Undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase. Pfam: Glyco_transf_28 (PF03033.27), Glyco_tran_28_C (PF04101.23). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WJK9
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase |
| EC (curated) |
EC 2.4.1.227
|
| Curated function | Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc-(pentapeptide)GlcNAc (lipid intermediate II). |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
M Cell wall / membrane / envelope biogenesis
|
|---|---|
| Preferred name | murG |
| eggNOG description | Cell wall formation. Catalyzes the transfer of a GlcNAc subunit on undecaprenyl-pyrophosphoryl-MurNAc-pentapeptide (lipid intermediate I) to form undecaprenyl-pyrophosphoryl-MurNAc- (pentapeptide)GlcNAc (lipid intermediate II) |
| Orthologous group | COG0707 |
| EC number |
EC 2.4.1.227
|
| KEGG orthology |
K02563
|
| KEGG pathways |
map00550, map01100, map01502, map04112
|
| CAZy family |
GT28
|
| Gene Ontology (2) |
GO:0008150, GO:0040007
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 1.113 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 3 synonymous, 8 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Glyco_transf_28 | PF03033.27 | 3.7e-35 | 38–185 | Glycosyltransferase family 28 N-terminal domain |
Glyco_tran_28_C | PF04101.23 | 8.3e-43 | 231–392 | Glycosyltransferase family 28 C-terminal domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: murD (UDP-N-acetylmuramoylalanine--D-glutamate ligase), high confidence from genomic context alone (score 999 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2155c murD |
UDP-N-acetylmuramoylalanine--D-glutamate ligase | 999 | 999 ctx | neighborhood:876 cooccurence:713 coexpression:968 textmining:894 |
Rv2156c murX |
phospho-N-acetylmuramoyl-pentappeptidetransferase | 999 | 998 ctx | neighborhood:876 cooccurence:713 coexpression:493 database:900 textmining:798 |
Rv2157c murF |
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase | 999 | 996 ctx | neighborhood:876 cooccurence:703 coexpression:903 textmining:944 |
Rv2152c murC |
UDP-N-acetylmuramate--alanine ligase | 999 | 996 ctx | neighborhood:882 cooccurence:658 coexpression:918 textmining:879 |
Rv2154c ftsW |
lipid II flippase FtsW | 999 | 995 ctx | neighborhood:881 cooccurence:462 coexpression:893 textmining:902 |
Rv2151c ftsQ |
cell division protein FtsQ | 998 | 995 ctx | neighborhood:881 coexpression:962 textmining:746 |
Rv2158c murE |
UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase | 997 | 980 ctx | neighborhood:876 cooccurence:664 coexpression:430 textmining:855 |
Rv3712 |
ligase | 961 | 956 | coexpression:416 database:900 |
Rv2150c ftsZ |
cell division protein FtsZ | 981 | 908 ctx | neighborhood:566 cooccurence:416 experimental:563 textmining:801 |
Rv3713 cobQ2 |
cobyric acid synthase CobQ | 907 | 902 | database:900 |
Rv2981c ddlA |
D-alanine--D-alanine ligase | 964 | 822 ctx | cooccurence:427 coexpression:678 textmining:808 |
Rv2163c pbpB |
penicillin-binding membrane protein PbpB | 964 | 787 ctx | neighborhood:544 cooccurence:499 textmining:839 |
Rv2894c xerC |
tyrosine recombinase XerC | 780 | 770 | coexpression:731 |
Rv2149c yfiH |
laccase domain-containing protein | 904 | 767 ctx | neighborhood:715 textmining:607 |
Rv0482 murB |
UDP-N-acetylenolpyruvoylglucosamine reductase | 969 | 727 ctx | cooccurence:512 textmining:892 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol-N-acetylglucosamine transferase
- MTBC0 PGAP product: undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase
- Pfam (hmmscan --cut_ga): Glyco_transf_28 PF03033.27 (E=4e-35), Glyco_tran_28_C PF04101.23 (E=8e-43)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216669.1)
- Domains: Pfam-A via hmmscan --cut_ga — Glyco_transf_28 (PF03033.27), Glyco_tran_28_C (PF04101.23)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0707 - Curated reference: UniProt P9WJK9 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
74 functional partner(s); context anchor
murD - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_002289|Rv2153c|murG MKDTVSQPAGGRGATAPRPADAASPSCGSSPSADSLSVVLAGGGTAGHVEPAMAVADALVALDPRVRITALGTLRGLETRLVPQRGYHLELITAVPMPRKPGGDLARLPSRVWRAVREARDVLDDVDADVVVGFGGYVALPAYLAARGLPLPPRRRRRIPVVIHEANARAGLANRVGAHTADRVLSAVPDSGLRRAEVVGVPVRASIAALDRAVLRAEARAHFGFPDDARVLLVFGGSQGAVSLNRAVSGAAADLAAAGVCVLHAHGPQNVLELRRRAQGDPPYVAVPYLDRMELAYAAADLVICRAGAMTVAEVSAVGLPAIYVPLPIGNGEQRLNALPVVNAGGGMVVADAALTPELVARQVAGLLTDPARLAAMTAAAARVGHRDAAGQVARAALAVATGAGARTTT