Rv2147c Resolved · high auto-curated
H37Rv Rv2147c · MTBC0 - ·
241 aa · 2406118–2406843 (-) ·
RefSeq NP_216663.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | cell division protein SepF |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | Cell division protein SepF. Pfam: SepF (PF04472.18). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
P9WGJ5
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Cell division protein SepF |
| Curated function | Cell division protein that is part of the divisome complex and is recruited early to the Z-ring. Probably stimulates Z-ring formation, perhaps through the cross-linking of FtsZ protofilaments. Its function overlaps with FtsA. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
D Cell cycle control, cell division, chromosome partitioning
|
|---|---|
| Preferred name | sepF |
| eggNOG description | Cell division protein that is part of the divisome complex and is recruited early to the Z-ring. Probably stimulates Z-ring formation, perhaps through the cross-linking of FtsZ protofilaments. Its function overlaps with FtsA |
| Orthologous group | COG1799 |
| KEGG orthology |
K09772
|
| Gene Ontology (6) |
GO:0005575, GO:0005623, GO:0005886, GO:0016020, GO:0044464, GO:0071944
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.621 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 1 synonymous, 2 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
SepF | PF04472.18 | 4.3e-24 | 153–224 | Cell division protein SepF |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: ftsZ (cell division protein FtsZ), high confidence from genomic context alone (score 987 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2150c ftsZ |
cell division protein FtsZ | 997 | 987 ctx | neighborhood:812 coexpression:664 experimental:814 textmining:842 |
Rv2148c hyp |
hypothetical protein | 969 | 965 ctx | neighborhood:831 coexpression:800 |
Rv2146c |
transmembrane protein | 953 | 946 ctx | neighborhood:747 coexpression:795 |
Rv2149c yfiH |
laccase domain-containing protein | 968 | 828 ctx | neighborhood:816 textmining:823 |
Rv2145c wag31 |
cell wall synthesis protein Wag31 | 954 | 804 ctx | neighborhood:659 coexpression:450 textmining:775 |
Rv2157c murF |
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase | 833 | 762 ctx | neighborhood:559 coexpression:412 |
Rv2151c ftsQ |
cell division protein FtsQ | 980 | 643 ctx | neighborhood:563 textmining:948 |
Rv2144c |
transmembrane protein | 641 | 641 ctx | neighborhood:632 |
Rv2154c ftsW |
lipid II flippase FtsW | 964 | 638 ctx | neighborhood:563 textmining:905 |
Rv2153c murG |
UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol-N-acetylglucosamine transferase | 851 | 622 ctx | neighborhood:563 textmining:623 |
Rv2156c murX |
phospho-N-acetylmuramoyl-pentappeptidetransferase | 622 | 622 ctx | neighborhood:559 |
Rv2152c murC |
UDP-N-acetylmuramate--alanine ligase | 842 | 580 ctx | neighborhood:563 textmining:639 |
Rv0003 recF |
DNA replication/repair protein RecF | 685 | 573 ctx | cooccurence:553 |
Rv2155c murD |
UDP-N-acetylmuramoylalanine--D-glutamate ligase | 830 | 565 ctx | neighborhood:559 textmining:625 |
Rv2158c murE |
UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase | 724 | 559 ctx | neighborhood:559 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): cell division protein SepF
- Pfam (hmmscan --cut_ga): SepF PF04472.18 (E=4e-24)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216663.1)
- Domains: Pfam-A via hmmscan --cut_ga — SepF (PF04472.18)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1799 - Curated reference: UniProt P9WGJ5 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
63 functional partner(s); context anchor
ftsZ - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv2147c| MNSHCSHTFITDNRSPRARRGHAMSTLHKVKAYFGMAPMEDYDDEYYDDRAPSRGYARPRFDDDYGRYDGRDYDDARSDSRGDLRGEPADYPPPGYRGGYADEPRFRPREFDRAEMTRPRFGSWLRNSTRGALAMDPRRMAMMFEDGHPLSKITTLRPKDYSEARTIGERFRDGSPVIMDLVSMDNADAKRLVDFAAGLAFALRGSFDKVATKVFLLSPADVDVSPEERRRIAETGFYAYQ