ftsZ Resolved · high auto-curated
H37Rv Rv2150c · MTBC0 mtbc0_002286 ·
379 aa · 2435764–2436903 (-) ·
RefSeq NP_216666.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | cell division protein FtsZ |
|---|---|
| MTBC0 PGAP re-annotation | cell division protein FtsZ |
| Revised (this work) | Cell division protein FtsZ. Pfam: Tubulin (PF00091.32), FtsZ_C (PF12327.14). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WN95
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Cell division protein FtsZ |
| Curated function | Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
D Cell cycle control, cell division, chromosome partitioning
|
|---|---|
| Preferred name | ftsZ |
| eggNOG description | Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity |
| Orthologous group | COG0206 |
| KEGG orthology |
K03531
|
| KEGG pathways |
map04112
|
| Gene Ontology (74) |
GO:0000166, GO:0000287, GO:0000910, GO:0000921, GO:0000935, GO:0001882, GO:0001883, GO:0003674, GO:0003824, GO:0003924, GO:0005488, GO:0005525 +62 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.513 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 2 synonymous, 3 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Tubulin | PF00091.32 | 7.5e-49 | 11–169 | Tubulin/FtsZ family, GTPase domain |
FtsZ_C | PF12327.14 | 3.0e-35 | 219–313 | FtsZ family, C-terminal domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: sepF (cell division protein SepF), high confidence from genomic context alone (score 987 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2147c sepF |
cell division protein SepF | 997 | 987 ctx | neighborhood:812 coexpression:664 experimental:814 textmining:842 |
Rv2153c murG |
UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol-N-acetylglucosamine transferase | 981 | 908 ctx | neighborhood:566 cooccurence:416 experimental:563 textmining:801 |
Rv2148c hyp |
hypothetical protein | 901 | 892 ctx | neighborhood:879 |
Rv2149c yfiH |
laccase domain-containing protein | 899 | 886 ctx | neighborhood:879 |
Rv2154c ftsW |
lipid II flippase FtsW | 997 | 872 ctx | neighborhood:566 experimental:563 textmining:978 |
Rv2146c |
transmembrane protein | 850 | 844 ctx | neighborhood:569 coexpression:652 |
Rv2152c murC |
UDP-N-acetylmuramate--alanine ligase | 953 | 795 ctx | neighborhood:566 textmining:784 |
Rv2151c ftsQ |
cell division protein FtsQ | 991 | 764 ctx | neighborhood:566 coexpression:478 textmining:965 |
Rv2158c murE |
UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase | 908 | 760 ctx | neighborhood:561 textmining:635 |
Rv2157c murF |
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase | 876 | 748 ctx | neighborhood:561 textmining:529 |
Rv2156c murX |
phospho-N-acetylmuramoyl-pentappeptidetransferase | 762 | 731 ctx | neighborhood:561 |
Rv2155c murD |
UDP-N-acetylmuramoylalanine--D-glutamate ligase | 923 | 719 ctx | neighborhood:561 textmining:738 |
Rv1308 atpA |
ATP synthase subunit alpha | 730 | 647 | coexpression:646 |
Rv2163c pbpB |
penicillin-binding membrane protein PbpB | 987 | 615 ctx | neighborhood:505 textmining:969 |
Rv3012c gatC |
glutamyl-tRNA(GLN) amidotransferase subunit C | 657 | 606 | coexpression:606 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: cell division protein FtsZ
- MTBC0 PGAP product: cell division protein FtsZ
- Pfam (hmmscan --cut_ga): Tubulin PF00091.32 (E=7e-49), FtsZ_C PF12327.14 (E=3e-35)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216666.1)
- Domains: Pfam-A via hmmscan --cut_ga — Tubulin (PF00091.32), FtsZ_C (PF12327.14)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0206 - Curated reference: UniProt P9WN95 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
172 functional partner(s); context anchor
sepF - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_002286|Rv2150c|ftsZ MTPPHNYLAVIKVVGIGGGGVNAVNRMIEQGLKGVEFIAINTDAQALLMSDADVKLDVGRDSTRGLGAGADPEVGRKAAEDAKDEIEELLRGADMVFVTAGEGGGTGTGGAPVVASIARKLGALTVGVVTRPFSFEGKRRSNQAENGIAALRESCDTLIVIPNDRLLQMGDAAVSLMDAFRSADEVLLNGVQGITDLITTPGLINVDFADVKGIMSGAGTALMGIGSARGEGRSLKAAEIAINSPLLEASMEGAQGVLMSIAGGSDLGLFEINEAASLVQDAAHPDANIIFGTVIDDSLGDEVRVTVIAAGFDVSGPGRKPVMGETGGAHRIESAKAGKLTSTLFEPVDAVSVPLHTNGATLSIGGDDDDVDVPPFMRR