ftsW Family assigned · medium auto-curated
H37Rv Rv2154c · MTBC0 mtbc0_002290 ·
524 aa · 2440727–2442301 (-) ·
RefSeq NP_216670.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | lipid II flippase FtsW |
|---|---|
| MTBC0 PGAP re-annotation | putative lipid II flippase FtsW |
| Revised (this work) | Putative lipid II flippase FtsW. Pfam: FTSW_RODA_SPOVE (PF01098.25). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WN97
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Probable peptidoglycan glycosyltransferase FtsW |
| EC (curated) |
EC 2.4.99.28
|
| Curated function | Peptidoglycan polymerase that is essential for cell division. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
D Cell cycle control, cell division, chromosome partitioning
|
|---|---|
| Preferred name | ftsW |
| eggNOG description | Belongs to the SEDS family |
| Orthologous group | COG0772 |
| EC number |
EC 2.4.1.227
|
| KEGG orthology |
K02563, K03588
|
| KEGG pathways |
map00550, map01100, map01502, map04112
|
| CAZy family |
GT28
|
| Gene Ontology (2) |
GO:0008150, GO:0040007
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.485 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 4 synonymous, 5 missense, 0 nonsense, 1 frameshift |
| Disruption | 1 distinct premature-stop/frameshift site(s); most common in 0.12% of strains (175) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
FTSW_RODA_SPOVE | PF01098.25 | 3.4e-128 | 58–412 | Cell cycle protein |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: murG (UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol-N-acetylglucosamine transferase), high confidence from genomic context alone (score 995 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2153c murG |
UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol-N-acetylglucosamine transferase | 999 | 995 ctx | neighborhood:881 cooccurence:462 coexpression:893 textmining:902 |
Rv2152c murC |
UDP-N-acetylmuramate--alanine ligase | 998 | 995 ctx | neighborhood:881 cooccurence:682 coexpression:876 textmining:753 |
Rv2151c ftsQ |
cell division protein FtsQ | 999 | 989 ctx | neighborhood:881 coexpression:843 experimental:443 textmining:958 |
Rv2157c murF |
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase | 994 | 977 ctx | neighborhood:876 cooccurence:665 coexpression:429 textmining:756 |
Rv2156c murX |
phospho-N-acetylmuramoyl-pentappeptidetransferase | 984 | 976 ctx | neighborhood:876 cooccurence:666 coexpression:465 |
Rv2155c murD |
UDP-N-acetylmuramoylalanine--D-glutamate ligase | 992 | 972 ctx | neighborhood:876 cooccurence:472 coexpression:607 textmining:741 |
Rv2163c pbpB |
penicillin-binding membrane protein PbpB | 998 | 961 ctx | neighborhood:544 cooccurence:753 experimental:589 textmining:964 |
Rv2158c murE |
UDP-N-acetylmuramoylalanyl-D-glutamate--2,6-diaminopimelate ligase | 982 | 944 ctx | neighborhood:876 cooccurence:454 textmining:694 |
Rv0016c pbpA |
penicillin-binding protein PbpA | 944 | 907 ctx | cooccurence:649 experimental:589 textmining:431 |
Rv2150c ftsZ |
cell division protein FtsZ | 997 | 872 ctx | neighborhood:566 experimental:563 textmining:978 |
Rv3209 hyp |
hypothetical protein | 793 | 793 | coexpression:793 |
Rv1477 ripA |
peptidoglycan endopeptidase RipA | 840 | 791 | coexpression:791 |
Rv2864c |
penicillin-binding lipoprotein | 800 | 787 | experimental:589 |
Rv2149c yfiH |
laccase domain-containing protein | 915 | 770 ctx | neighborhood:707 textmining:645 |
Rv1157c hyp |
hypothetical protein | 764 | 764 | coexpression:761 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: lipid II flippase FtsW
- MTBC0 PGAP product: putative lipid II flippase FtsW
- Pfam (hmmscan --cut_ga): FTSW_RODA_SPOVE PF01098.25 (E=3e-128)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216670.1)
- Domains: Pfam-A via hmmscan --cut_ga — FTSW_RODA_SPOVE (PF01098.25)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0772 - Curated reference: UniProt P9WN97 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
82 functional partner(s); context anchor
murG - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_002290|Rv2154c|ftsW MLTRLLRRGTSDTDGSQTRGAEPVEGQRTGPEEASNPGSARPRTRFGAWLGRPMTSFHLIIAVAALLTTLGLIMVLSASAVRSYDDDGSAWVIFGKQVLWTLVGLIGGYVCLRMSVRFMRRIAFSGFAITIVMLVLVLVPGIGKEANGSRGWFVVAGFSMQPSELAKMAFAIWGAHLLAARRMERASLREMLIPLVPAAVVALALIVAQPDLGQTVSMGIILLGLLWYAGLPLRVFLSSLAAVVVSAAILAVSAGYRSDRVRSWLNPENDPQDSGYQARQAKFALAQGGIFGDGLGQGVAKWNYLPNAHNDFIFAIIGEELGLVGALGLLGLFGLFAYTGMRIASRSADPFLRLLTATTTLWVLGQAFINIGYVIGLLPVTGLQLPLISAGGTSTAATLSLIGIIANAARHEPEAVAALRAGRDDKVNRLLRLPLPEPYLPPRLEAFRDRKRANPQPAQTQPARKTPRTAPGQPARQMGLPPRPGSPRTADPPVRRSVHHGAGQRYAGQRRTRRVRALEGQRYG