esxW Resolved · high auto-curated
H37Rv Rv3620c · MTBC0 mtbc0_003837 ·
98 aa · 4083999–4084295 (-) ·
RefSeq NP_218137.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | ESAT-6 like protein EsxW |
|---|---|
| MTBC0 PGAP re-annotation | type VII secretion system protein EsxW |
| Revised (this work) | Type VII secretion system protein EsxW. Pfam: WXG100 (PF06013.19). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WNI3
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | ESAT-6-like protein EsxW |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| Preferred name | esxP |
| eggNOG description | Belongs to the WXG100 family |
| Orthologous group | COG4842 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.271 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 2 synonymous, 2 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
WXG100 | PF06013.19 | 6.1e-20 | 5–90 | Proteins of 100 residues with WXG |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: esxV (ESAT-6 like protein EsxV), high confidence from genomic context alone (score 957 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3619c esxV |
ESAT-6 like protein EsxV | 993 | 957 ctx | neighborhood:703 coexpression:860 textmining:853 |
Rv1037c esxI |
ESAT-6 like protein EsxI | 955 | 942 | coexpression:799 experimental:715 |
Rv2346c esxO |
ESAT-6 like protein EsxO | 954 | 865 | coexpression:860 textmining:676 |
Rv1793 esxN |
ESAT-6 like protein EsxN | 906 | 865 | coexpression:860 |
Rv1198 esxL |
ESAT-6 like protein EsxL | 894 | 865 | coexpression:860 |
Rv2347c esxP |
ESAT-6 like protein EsxP | 861 | 860 | coexpression:860 |
Rv1197 esxK |
ESAT-6 like protein EsxK | 860 | 860 | coexpression:860 |
Rv1038c esxJ |
ESAT-6 like protein EsxJ | 860 | 860 | coexpression:860 |
Rv3648c cspA |
cold shock protein A | 839 | 806 | coexpression:804 |
Rv3874 esxB |
ESAT-6-like protein EsxB | 926 | 802 | coexpression:802 textmining:644 |
Rv3875 esxA |
ESAT-6 protein EsxA | 924 | 800 | coexpression:800 textmining:640 |
Rv1196 PPE18 |
PPE family protein PPE18 | 882 | 799 | coexpression:799 textmining:438 |
Rv0685 tuf |
elongation factor Tu | 731 | 731 | coexpression:731 |
Rv3621c PPE65 |
PPE family protein PPE65 | 612 | 612 ctx | neighborhood:608 |
Rv3622c PE32 |
PE family protein PE32 | 604 | 604 ctx | neighborhood:604 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: ESAT-6 like protein EsxW
- MTBC0 PGAP product: type VII secretion system protein EsxW
- Pfam (hmmscan --cut_ga): WXG100 PF06013.19 (E=6e-20)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_218137.1)
- Domains: Pfam-A via hmmscan --cut_ga — WXG100 (PF06013.19)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG4842 - Curated reference: UniProt P9WNI3 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
22 functional partner(s); context anchor
esxV - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_003837|Rv3620c|esxW MTSRFMTDPHAMRDMAGRFEVHAQTVEDEARRMWASAQNISGAGWSGMAEATSLDTMTQMNQAFRNIVNMLHGVRDGLVRDANNYEQQEQASQQILSS