Rv3631 Family assigned · medium auto-curated
H37Rv Rv3631 · MTBC0 mtbc0_003848 ·
241 aa · 4094218–4094943 (+) ·
RefSeq NP_218148.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | transferase |
|---|---|
| MTBC0 PGAP re-annotation | glycosyltransferase family 2 protein |
| Revised (this work) | Glycosyltransferase family 2 protein. Pfam: Glycos_transf_2 (PF00535.33). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
O06376
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Possible transferase |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
M Cell wall / membrane / envelope biogenesis
|
|---|---|
| eggNOG description | glycosyl transferase |
| Orthologous group | COG0463 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.166 · strong purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 2 synonymous, 1 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Glycos_transf_2 | PF00535.33 | 4.1e-26 | 16–148 | Glycosyl transferase family 2 |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv3632 (membrane protein), high confidence from genomic context alone (score 971 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3632 |
membrane protein | 996 | 971 ctx | neighborhood:881 cooccurence:760 textmining:870 |
Rv3630 |
integral membrane protein | 982 | 909 ctx | neighborhood:818 cooccurence:472 textmining:813 |
Rv3629c |
integral membrane protein | 956 | 677 ctx | neighborhood:674 textmining:870 |
Rv1510 hyp |
hypothetical protein | 627 | 607 ctx | cooccurence:463 |
Rv3779 |
transmembrane protein | 849 | 584 ctx | cooccurence:568 textmining:653 |
Rv3634c galE1 |
UDP-glucose 4-epimerase | 913 | 568 ctx | cooccurence:459 textmining:809 |
Rv3633 hyp |
hypothetical protein | 929 | 477 ctx | neighborhood:468 textmining:870 |
Rv3781 rfbE |
O-antigen/lipopolysaccharide ABC transporter ATP-binding protein RfbE | 478 | 450 | |
Rv3784 |
dTDP-glucose 4,6-dehydratase | 662 | 426 | textmining:436 |
Rv0539 |
dolichyl-phosphate sugar synthase | 911 | 346 | textmining:870 |
Rv3782 glfT1 |
galactofuranosyl transferase GlfT | 422 | 192 | |
Rv3468c |
dTDP-glucose 4,6-dehydratase | 709 | 178 | textmining:661 |
Rv0536 galE3 |
UDP-glucose 4-epimerase GalE | 889 | 158 | textmining:874 |
Rv0540 hyp |
hypothetical protein | 874 | 77 | textmining:870 |
Rv0535 pnp |
5'-methylthioadenosine phosphorylase | 867 | 65 | textmining:864 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: transferase
- MTBC0 PGAP product: glycosyltransferase family 2 protein
- Pfam (hmmscan --cut_ga): Glycos_transf_2 PF00535.33 (E=4e-26)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_218148.1)
- Domains: Pfam-A via hmmscan --cut_ga — Glycos_transf_2 (PF00535.33)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0463 - Curated reference: UniProt O06376 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
18 functional partner(s); context anchor
Rv3632 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_003848|Rv3631| MASKMDTETHYSDVWVVIPAFNEAAVIGKVVTDVRSVFDHVVCVDDGSTDGTGDIARRSGAHLVRHPINLGQGAAIQTGIEYARKQPGAQVFATFDGDGQHRVKDVAAMVDRLGAGDVDVVIGTRFGRPVGKASASRPPLMKRIVLQTGARLSRRGRRLGLTDTNNGLRVFNKTVADGLNITMSGMSHATEFIMLIAENHWRVAEEPVEVLYTEYSKSKGQPLLNGVNIIFDGFLRGRMPR