tuf Resolved · high auto-curated
H37Rv Rv0685 · MTBC0 - ·
396 aa · 784821–786011 (+) ·
RefSeq NP_215199.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | elongation factor Tu |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | Elongation factor Tu. Pfam: GTP_EFTU (PF00009.34), GTP_EFTU_D2 (PF03144.32), GTP_EFTU_D3 (PF03143.24). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
P9WNN1
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Elongation factor Tu |
| EC (curated) |
EC 3.6.5.3
|
| Curated function | GTP hydrolase that promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
J Translation, ribosomal structure and biogenesis
|
|---|---|
| Preferred name | tuf |
| eggNOG description | This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis |
| Orthologous group | COG0050 |
| KEGG orthology |
K02358
|
| Gene Ontology (31) |
GO:0001666, GO:0003674, GO:0005488, GO:0005515, GO:0005575, GO:0005576, GO:0005618, GO:0005622, GO:0005623, GO:0005737, GO:0005829, GO:0005886 +19 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
GTP_EFTU | PF00009.34 | 3.2e-64 | 10–202 | Elongation factor Tu GTP binding domain |
GTP_EFTU_D2 | PF03144.32 | 1.6e-19 | 226–296 | Elongation factor Tu domain 2 |
GTP_EFTU_D3 | PF03143.24 | 1.4e-38 | 301–394 | Elongation factor Tu C-terminal domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: rpsL (30S ribosomal protein S12), high confidence from genomic context alone (score 1000 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0682 rpsL |
30S ribosomal protein S12 | 999 | 1000 ctx | neighborhood:680 coexpression:969 experimental:928 textmining:654 |
Rv2889c tsf |
elongation factor EF-Ts | 999 | 1000 | coexpression:861 experimental:999 database:664 textmining:771 |
Rv0721 rpsE |
30S ribosomal protein S5 | 999 | 999 | coexpression:967 experimental:928 |
Rv0704 rplB |
50S ribosomal protein L2 | 999 | 999 | coexpression:968 experimental:928 |
Rv0723 rplO |
50S ribosomal protein L15 | 999 | 999 | coexpression:969 experimental:928 |
Rv0700 rpsJ |
30S ribosomal protein S10 | 999 | 999 | coexpression:966 experimental:928 |
Rv0708 rplP |
50S ribosomal protein L16 | 998 | 999 | coexpression:968 experimental:928 |
Rv0702 rplD |
50S ribosomal protein L4 | 998 | 999 | coexpression:962 experimental:928 |
Rv3456c rplQ |
50S ribosomal protein L17 | 998 | 999 | coexpression:973 experimental:928 database:404 |
Rv0706 rplV |
50S ribosomal protein L22 | 998 | 999 | coexpression:968 experimental:928 |
Rv0683 rpsG |
30S ribosomal protein S7 | 999 | 998 ctx | neighborhood:680 coexpression:865 experimental:928 textmining:727 |
Rv3461c rpmJ |
50S ribosomal protein L36 | 998 | 998 | coexpression:954 experimental:928 database:404 |
Rv0701 rplC |
50S ribosomal protein L3 | 998 | 998 | coexpression:931 experimental:928 |
Rv0703 rplW |
50S ribosomal protein L23 | 998 | 998 | coexpression:970 experimental:928 |
Rv0705 rpsS |
30S ribosomal protein S19 | 998 | 998 | coexpression:973 experimental:928 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): elongation factor Tu
- Pfam (hmmscan --cut_ga): GTP_EFTU PF00009.34 (E=3e-64), GTP_EFTU_D2 PF03144.32 (E=2e-19), GTP_EFTU_D3 PF03143.24 (E=1e-38)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215199.1)
- Domains: Pfam-A via hmmscan --cut_ga — GTP_EFTU (PF00009.34), GTP_EFTU_D2 (PF03144.32), GTP_EFTU_D3 (PF03143.24)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0050 - Curated reference: UniProt P9WNN1 (SwissProt, reviewed; Evidence at protein level)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
273 functional partner(s); context anchor
rpsL - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv0685|tuf MAKAKFQRTKPHVNIGTIGHVDHGKTTLTAAITKVLHDKFPDLNETKAFDQIDNAPEERQRGITINIAHVEYQTDKRHYAHVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTREHVLLARQVGVPYILVALNKADAVDDEELLELVEMEVRELLAAQEFDEDAPVVRVSALKALEGDAKWVASVEELMNAVDESIPDPVRETDKPFLMPVEDVFTITGRGTVVTGRVERGVINVNEEVEIVGIRPSTTKTTVTGVEMFRKLLDQGQAGDNVGLLLRGVKREDVERGQVVTKPGTTTPHTEFEGQVYILSKDEGGRHTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTNISVKLIQPVAMDEGLRFAIREGGRTVGAGRVTKIIK