esxA Resolved · high auto-curated
H37Rv Rv3875 · MTBC0 - ·
95 aa · 4352609–4352896 (+) ·
RefSeq YP_178023.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | ESAT-6 protein EsxA |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | ESAT-6 protein EsxA. Pfam: WXG100 (PF06013.19). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
P9WNK7
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | 6 kDa early secretory antigenic target |
| Curated function | A secreted protein that plays a number of roles in modulating the host's immune response to infection as well as being responsible for bacterial escape into the host cytoplasm. Acts as a strong host (human) T-cell antigen. Inhibits IL-12 p40 (IL12B) and TNF expression by infected host (mouse) macrophages, reduces the nitric oxide response by about 75%. In mice previously exposed to the bacterium, elicits high level of IFN-gamma production by T-cells upon subsequent challenge by M.tuberculosis, in the first phase of a protective immune response. Higher levels (1.6-3.3 uM) of recombinant protein. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| Preferred name | esxA |
| eggNOG description | Belongs to the WXG100 family |
| Orthologous group | COG4842 |
| KEGG orthology |
K14956
|
| KEGG pathways |
map05152
|
| Gene Ontology (72) |
GO:0002790, GO:0003674, GO:0005488, GO:0005515, GO:0005575, GO:0005576, GO:0005618, GO:0005622, GO:0005623, GO:0005737, GO:0005886, GO:0006810 +60 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
WXG100 | PF06013.19 | 9.1e-18 | 5–87 | Proteins of 100 residues with WXG |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: esxB (ESAT-6-like protein EsxB), high confidence from genomic context alone (score 1000 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3874 esxB |
ESAT-6-like protein EsxB | 999 | 1000 ctx | neighborhood:833 coexpression:853 experimental:999 textmining:997 |
Rv3877 eccD1 |
ESX-1 secretion system protein EccD1 | 971 | 893 ctx | neighborhood:570 cooccurence:754 textmining:745 |
Rv3870 eccCa1 |
ESX-1 secretion system protein EccCa | 946 | 857 ctx | neighborhood:416 cooccurence:656 textmining:642 |
Rv3869 eccB1 |
ESX-1 secretion system protein EccB | 900 | 822 ctx | cooccurence:731 textmining:461 |
Rv2346c esxO |
ESAT-6 like protein EsxO | 921 | 802 | coexpression:802 textmining:621 |
Rv3620c esxW |
ESAT-6 like protein EsxW | 924 | 800 | coexpression:800 textmining:640 |
Rv1038c esxJ |
ESAT-6 like protein EsxJ | 910 | 800 | coexpression:800 textmining:569 |
Rv1197 esxK |
ESAT-6 like protein EsxK | 903 | 800 | coexpression:800 textmining:537 |
Rv1198 esxL |
ESAT-6 like protein EsxL | 927 | 799 | coexpression:799 textmining:652 |
Rv2347c esxP |
ESAT-6 like protein EsxP | 930 | 798 | coexpression:798 textmining:672 |
Rv1793 esxN |
ESAT-6 like protein EsxN | 924 | 797 | coexpression:797 textmining:645 |
Rv3871 eccCb1 |
ESX-1 secretion system protein EccCb | 955 | 774 ctx | cooccurence:537 textmining:810 |
Rv3882c eccE1 |
ESX-1 secretion system protein EccE1 | 869 | 771 ctx | cooccurence:762 textmining:454 |
Rv3876 espI |
ESX-1 secretion-associated protein EspI | 954 | 754 ctx | neighborhood:592 textmining:822 |
Rv3619c esxV |
ESAT-6 like protein EsxV | 908 | 748 | coexpression:748 textmining:651 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): ESAT-6 protein EsxA
- Pfam (hmmscan --cut_ga): WXG100 PF06013.19 (E=9e-18)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq YP_178023.1)
- Domains: Pfam-A via hmmscan --cut_ga — WXG100 (PF06013.19)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG4842 - Curated reference: UniProt P9WNK7 (SwissProt, reviewed; Evidence at protein level)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
111 functional partner(s); context anchor
esxB - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv3875|esxA MTEQQWNFAGIEAAASAIQGNVTSIHSLLDEGKQSLTKLAAAWGGSGSEAYQGVQQKWDATATELNNALQNLARTISEAGQAMASTEGNVTGMFA