mec Resolved · high auto-curated
H37Rv Rv1334 · MTBC0 mtbc0_001431 ·
146 aa · 1513343–1513783 (+) ·
RefSeq NP_215850.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | [CysO |
|---|---|
| MTBC0 PGAP re-annotation | CysO-cysteine peptidase |
| Revised (this work) | CysO-cysteine peptidase. Pfam: Prok-JAB (PF14464.12). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WHS1
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | CysO-cysteine peptidase |
| EC (curated) |
EC 3.13.1.6
|
| Curated function | Protease that hydrolyzes the covalent CysO-cysteine adduct synthesized by CysM to release L-cysteine and regenerate CysO. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| Preferred name | mec |
| eggNOG description | metal-dependent protease of the Pad1 Jab1 superfamily |
| Orthologous group | COG1310 |
| EC number |
EC 3.13.1.6
|
| KEGG orthology |
K21140
|
| KEGG pathways |
map04122
|
| Gene Ontology (59) |
GO:0000096, GO:0000097, GO:0003674, GO:0003824, GO:0005488, GO:0005575, GO:0005622, GO:0005623, GO:0005737, GO:0005829, GO:0006082, GO:0006508 +47 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | n/a |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 0 synonymous, 4 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Prok-JAB | PF14464.12 | 1.9e-29 | 15–130 | Prokaryotic homologs of the JAB domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: cysM (O-phosphoserine sulfhydrylase), high confidence from genomic context alone (score 992 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2115c mpa |
proteasome-associated ATPase | 994 | 993 | coexpression:463 experimental:918 database:844 |
Rv1336 cysM |
O-phosphoserine sulfhydrylase | 991 | 992 ctx | neighborhood:847 cooccurence:439 coexpression:809 database:500 |
Rv1335 cysO |
sulfur carrier protein CysO | 991 | 991 ctx | neighborhood:851 cooccurence:715 coexpression:797 |
Rv3696c glpK |
glycerol kinase | 988 | 986 | experimental:908 database:844 |
Rv0707 rpsC |
30S ribosomal protein S3 | 958 | 957 | experimental:854 database:652 |
Rv0705 rpsS |
30S ribosomal protein S19 | 956 | 957 | experimental:868 database:652 |
Rv1329c dinG |
ATP-dependent helicase DinG | 950 | 948 ctx | neighborhood:750 database:774 |
Rv0683 rpsG |
30S ribosomal protein S7 | 948 | 948 | experimental:852 database:623 |
Rv0721 rpsE |
30S ribosomal protein S5 | 946 | 947 | experimental:834 database:644 |
Rv0710 rpsQ |
30S ribosomal protein S17 | 946 | 947 | experimental:838 database:652 |
Rv0700 rpsJ |
30S ribosomal protein S10 | 943 | 944 | experimental:839 database:644 |
Rv2785c rpsO |
30S ribosomal protein S15 | 950 | 943 | experimental:835 database:644 |
Rv2890c rpsB |
30S ribosomal protein S2 | 945 | 942 | experimental:827 database:605 |
Rv0718 rpsH |
30S ribosomal protein S8 | 942 | 940 | experimental:826 database:652 |
Rv0702 rplD |
50S ribosomal protein L4 | 942 | 939 | experimental:826 database:643 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: [CysO
- MTBC0 PGAP product: CysO-cysteine peptidase
- Pfam (hmmscan --cut_ga): Prok-JAB PF14464.12 (E=2e-29)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215850.1)
- Domains: Pfam-A via hmmscan --cut_ga — Prok-JAB (PF14464.12)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1310 - Curated reference: UniProt P9WHS1 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
171 functional partner(s); context anchor
cysM - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001431|Rv1334|mec MLLRKGTVYVLVIRADLVNAMVAHARRDHPDEACGVLAGPEGSDRPERHIPMTNAERSPTFYRLDSGEQLKVWRAMEDADEVPVVIYHSHTATEAYPSRTDVKLATEPDAHYVLVSTRDPHRHELRSYRIVDGAVTEEPVNVVEQY