Rv0774c Resolved · high auto-curated
H37Rv Rv0774c · MTBC0 mtbc0_000823 ·
303 aa · 871864–872775 (-) ·
RefSeq NP_215288.2
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | hypothetical protein |
|---|---|
| MTBC0 PGAP re-annotation | iron-stress inducible esterase |
| Revised (this work) | Iron-stress inducible esterase. Pfam: Esterase (PF00756.27). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
I6Y8R4
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Acyl-CoA:diacylglycerol acyltransferase |
| EC (curated) |
EC 2.3.1.122, EC 2.3.1.20
|
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| eggNOG description | esterase |
| Orthologous group | COG0627 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.732 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 3 synonymous, 6 missense, 0 nonsense, 2 frameshift |
| Disruption | 2 distinct premature-stop/frameshift site(s); most common in 0.43% of strains (618) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Esterase | PF00756.27 | 3.6e-07 | 87–260 | Putative esterase |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: ggtA (bifunctional cephalosporin acylase/gamma-glutamyltranspeptidase), medium confidence from genomic context alone (score 669 excluding text-mining). This association is the citable seed of a function hypothesis for this hypothetical protein.
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0773c ggtA |
bifunctional cephalosporin acylase/gamma-glutamyltranspeptidase | 668 | 669 ctx | neighborhood:664 |
Rv0775 hyp |
hypothetical protein | 632 | 633 ctx | neighborhood:633 |
Rv0272c hyp |
hypothetical protein | 646 | 625 ctx | cooccurence:625 |
Rv0412c glnX |
membrane protein | 608 | 593 | coexpression:407 |
Rv0290 eccD3 |
ESX-3 secretion system protein EccD | 548 | 549 ctx | cooccurence:544 |
Rv0761c adhB |
alcohol dehydrogenase B | 549 | 529 | coexpression:446 |
Rv2259 mscR |
S-nitrosomycothiol reductase MscR | 548 | 528 | coexpression:445 |
Rv0162c adhE1 |
zinc-type alcohol dehydrogenase subunit E | 546 | 526 | coexpression:443 |
Rv3086 adhD |
alcohol dehydrogenase D | 545 | 525 | coexpression:441 |
Rv0273c |
transcriptional regulator | 502 | 502 ctx | cooccurence:502 |
Rv1766 hyp |
hypothetical protein | 502 | 484 | coexpression:409 |
Rv0676c mmpL5 |
transmembrane transport protein MmpL5 | 464 | 445 ctx | cooccurence:445 |
Rv1598c hyp |
hypothetical protein | 438 | 438 ctx | cooccurence:438 |
Rv0450c mmpL4 |
transmembrane transport protein MmpL4 | 453 | 434 ctx | cooccurence:433 |
Rv0507 mmpL2 |
transmembrane transport protein MmpL2 | 446 | 427 ctx | cooccurence:427 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: hypothetical protein
- MTBC0 PGAP product: iron-stress inducible esterase
- Pfam (hmmscan --cut_ga): Esterase PF00756.27 (E=4e-07)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215288.2)
- Domains: Pfam-A via hmmscan --cut_ga — Esterase (PF00756.27)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0627 - Curated reference: UniProt I6Y8R4 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
31 functional partner(s); context anchor
ggtA - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_000823|Rv0774c| MMARMPELSRRAVLGLGAGTVLGATSAYAIDMLLQPRTSHAAPAAAIGTNVPLAPTPALDPAPPAQAAPTMSTGSFVSAARAGKMTNWAIARPPGQTQALRPVIALHGLGGSASAVMDGGVEQGLAQAVNAGLPPFAVVSVDGGSSYWHQRASGEDAGAMVLNELIPLLDTQRLDTSRVAFLGWSMGGYGALLLGSRLGPARTAAICAVSPALWLSAGSVAPGSFDGPDDWSANSVFGLPALGSIPIRVDCGNSDPFYAATKQFVAQLPHPPAGGFSPGGHNGGFWSAQLPAELTWFAPLLTG