aldA Resolved · high auto-curated
H37Rv Rv0768 · MTBC0 mtbc0_000817 ·
489 aa · 865336–866805 (+) ·
RefSeq NP_215282.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | aldehyde dehydrogenase AldA |
|---|---|
| MTBC0 PGAP re-annotation | aldehyde dehydrogenase |
| Revised (this work) | Aldehyde dehydrogenase. Pfam: Aldedh (PF00171.28). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
I6X9R9
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Probable aldehyde dehydrogenase NAD dependent AldA |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
C Energy production and conversion
|
|---|---|
| Preferred name | aldA |
| eggNOG description | Belongs to the aldehyde dehydrogenase family |
| Orthologous group | COG1012 |
| EC number |
EC 1.2.1.3
|
| KEGG orthology |
K00128
|
| KEGG pathways |
map00010, map00053, map00071, map00280, map00310, map00330, map00340, map00380, map00410, map00561, map00620, map00625, map00903, map00981, map01100, map01110, map01120, map01130
|
| KEGG modules |
M00135
|
| Gene Ontology (54) |
GO:0000166, GO:0003674, GO:0003824, GO:0004029, GO:0004030, GO:0005488, GO:0005575, GO:0005622, GO:0005623, GO:0005737, GO:0005739, GO:0005759 +42 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.177 · strong purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 10 synonymous, 5 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Aldedh | PF00171.28 | 9.7e-145 | 22–483 | Aldehyde dehydrogenase family |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv0765c (oxidoreductase), high confidence from genomic context alone (score 958 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0765c |
oxidoreductase | 958 | 958 ctx | neighborhood:758 coexpression:835 |
Rv0770 |
oxidoreductase | 957 | 956 ctx | neighborhood:746 coexpression:832 |
Rv0761c adhB |
alcohol dehydrogenase B | 954 | 952 ctx | neighborhood:438 database:900 |
Rv0766c cyp123 |
cytochrome P450 Cyp123 | 953 | 952 ctx | neighborhood:758 coexpression:807 |
Rv3667 acs |
acetyl-CoAsynthetase | 950 | 947 | coexpression:418 database:900 |
Rv0764c cyp51 |
lanosterol 14-alpha demethylase | 943 | 943 ctx | neighborhood:758 coexpression:736 |
Rv0771 |
4-carboxymuconolactone decarboxylase | 941 | 939 ctx | neighborhood:746 coexpression:736 |
Rv2589 gabT |
4-aminobutyrate aminotransferase | 937 | 932 | database:900 |
Rv0753c mmsA |
methylmalonate-semialdehyde dehydrogenase | 929 | 927 | database:900 |
Rv0409 ackA |
acetate kinase | 930 | 922 | database:900 |
Rv0860 fadB |
fatty oxidation protein FadB | 925 | 921 | database:900 |
Rv1530 adh |
alcohol dehydrogenase | 924 | 920 | database:900 |
Rv0162c adhE1 |
zinc-type alcohol dehydrogenase subunit E | 924 | 920 | database:900 |
Rv3432c gadB |
glutamate decarboxylase GadB | 921 | 916 | database:900 |
Rv1862 adhA |
alcohol dehydrogenase A | 918 | 914 | database:900 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: aldehyde dehydrogenase AldA
- MTBC0 PGAP product: aldehyde dehydrogenase
- Pfam (hmmscan --cut_ga): Aldedh PF00171.28 (E=1e-144)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215282.1)
- Domains: Pfam-A via hmmscan --cut_ga — Aldedh (PF00171.28)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1012 - Curated reference: UniProt I6X9R9 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
93 functional partner(s); context anchor
Rv0765c - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_000817|Rv0768|aldA MALWGDGISALLIDGKLSDGRAGTFPTVNPATEEVLGVAADADAEDMGRAIEAARRAFDSTDWSRNTELRVRCVRQLRDAMQQHVEELRELTISEVGAPRMLTASAQLEGPVGDLSFAADTAESYPWKQDLGEASPLGIATRRTLAREAVGVVGAITPWNFPHQINLAKLGPALAAGNTVVLKPAPDTPWCAAALGEIIVEHTDFPPGVVNIVTSSSHALGALLAKDPRVDMISFTGSTATGRAVMADAAATIKKVFLELGGKSAFVVLDDADLAAASAVSAFSACMHAGQGCAITTRLVVPRARYEEAVAIAAATMSSIRPGDPNDPGTVCGPLISARQRDRVQGYLDLAVAEGGRFACGGARPADREVGFYIEPTVIAGLTNDARVAREEIFGPVLTVIAHDGDDDAVRIANDSPYGLSGTVYGADPQRAARIASRLRVGTVNVNGGVWYCADAPFGGYKQSGIGREMGLLGFEEYLEAKLIATAAN