Rv0765c Family assigned · medium auto-curated
H37Rv Rv0765c · MTBC0 mtbc0_000814 ·
275 aa · 862461–863288 (-) ·
RefSeq NP_215279.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | oxidoreductase |
|---|---|
| MTBC0 PGAP re-annotation | SDR family oxidoreductase |
| Revised (this work) | SDR family oxidoreductase. Pfam: adh_short (PF00106.32), KR (PF08659.17), adh_short_C2 (PF13561.13). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
I6WZD9
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Probable oxidoreductase |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| eggNOG description | Dehydrogenase |
| Orthologous group | COG4221 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | n/a |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 0 synonymous, 3 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
adh_short | PF00106.32 | 7.6e-47 | 12–203 | short chain dehydrogenase |
KR | PF08659.17 | 2.7e-11 | 12–174 | KR domain |
adh_short_C2 | PF13561.13 | 3.3e-38 | 17–238 | Enoyl-(Acyl carrier protein) reductase |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: cyp51 (lanosterol 14-alpha demethylase), high confidence from genomic context alone (score 989 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0764c cyp51 |
lanosterol 14-alpha demethylase | 989 | 989 ctx | neighborhood:882 cooccurence:406 coexpression:860 |
Rv0766c cyp123 |
cytochrome P450 Cyp123 | 982 | 983 ctx | neighborhood:882 coexpression:836 |
Rv0762c hyp |
hypothetical protein | 981 | 981 ctx | neighborhood:882 cooccurence:673 coexpression:553 |
Rv0763c |
ferredoxin | 979 | 979 ctx | neighborhood:882 cooccurence:482 coexpression:687 |
Rv0767c |
HTH-type transcriptional regulator | 966 | 965 ctx | neighborhood:881 cooccurence:708 |
Rv0768 aldA |
aldehyde dehydrogenase AldA | 958 | 958 ctx | neighborhood:758 coexpression:835 |
Rv0770 |
oxidoreductase | 951 | 949 ctx | neighborhood:677 coexpression:802 |
Rv0771 |
4-carboxymuconolactone decarboxylase | 866 | 862 ctx | neighborhood:677 cooccurence:488 |
Rv0769 |
oxidoreductase | 808 | 808 ctx | neighborhood:731 |
Rv0761c adhB |
alcohol dehydrogenase B | 774 | 774 ctx | neighborhood:774 |
Rv0311 hyp |
hypothetical protein | 771 | 772 ctx | cooccurence:714 |
Rv1714 |
oxidoreductase | 666 | 666 ctx | cooccurence:657 |
Rv3502c |
3-oxoacyl-ACP reductase | 645 | 646 ctx | cooccurence:633 |
Rv0760c hyp |
hypothetical protein | 569 | 569 ctx | neighborhood:566 |
Rv1175c fadH |
NADPH dependent 2,4-dienoyl-CoA reductase FadH | 549 | 533 ctx | neighborhood:522 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: oxidoreductase
- MTBC0 PGAP product: SDR family oxidoreductase
- Pfam (hmmscan --cut_ga): adh_short PF00106.32 (E=8e-47), KR PF08659.17 (E=3e-11), adh_short_C2 PF13561.13 (E=3e-38)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215279.1)
- Domains: Pfam-A via hmmscan --cut_ga — adh_short (PF00106.32), KR (PF08659.17), adh_short_C2 (PF13561.13)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG4221 - Curated reference: UniProt I6WZD9 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
17 functional partner(s); context anchor
cyp51 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_000814|Rv0765c| MPRFEPHPARRTTVVAGASSGIGAATATELAGRGFPVALGARRMDKLAELVDKIRADGGEAVAFPLDVTDPESVKSFVAQTVEALGEVELLVSSAGDMLPGQLHEVSTEAFAEQVQIHLVGANRLATAVLPAMVARRRGDLIFVGSDVGLRQRPHMGAYGAAKAGLAAMVTNLQMELEGTGVRASIVHPGPTLTGMGWQLSAEQVGPMLADWAKWGQARHNYFLRPSDLARAIAFVAETPRGCVVVNMEIQPEAPLRDAPAHRQKLVLGEEGMPG