Rv0775 Family assigned · medium auto-curated
H37Rv Rv0775 · MTBC0 mtbc0_000824 ·
207 aa · 872831–873454 (+) ·
RefSeq NP_215289.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | hypothetical protein |
|---|---|
| MTBC0 PGAP re-annotation | TetR/AcrR family transcriptional regulator |
| Revised (this work) | TetR/AcrR family transcriptional regulator. Pfam: TetR_N (PF00440.30), TetR_C_31 (PF17940.7). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P71830
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | HTH tetR-type domain-containing protein |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
K Transcription
|
|---|---|
| eggNOG description | tetR family |
| Orthologous group | COG3226 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | n/a |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 0 synonymous, 5 missense, 0 nonsense, 1 frameshift |
| Disruption | 1 distinct premature-stop/frameshift site(s); most common in 0.76% of strains (1104) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
TetR_N | PF00440.30 | 1.5e-07 | 18–63 | Bacterial regulatory proteins, tetR family |
TetR_C_31 | PF17940.7 | 2.2e-16 | 88–195 | Tetracyclin repressor-like, C-terminal domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv3066 (DeoR family transcriptional regulator), high confidence from genomic context alone (score 728 excluding text-mining). This association is the citable seed of a function hypothesis for this hypothetical protein.
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2360c hyp |
hypothetical protein | 737 | 737 ctx | cooccurence:734 |
Rv3066 |
DeoR family transcriptional regulator | 727 | 728 ctx | cooccurence:724 |
Rv1486c hyp |
hypothetical protein | 646 | 646 ctx | cooccurence:644 |
Rv1632c hyp |
hypothetical protein | 634 | 634 ctx | cooccurence:631 |
Rv0774c hyp |
hypothetical protein | 632 | 633 ctx | neighborhood:633 |
Rv0556 |
transmembrane protein | 630 | 630 ctx | cooccurence:628 |
Rv2732c |
transmembrane protein | 620 | 620 ctx | cooccurence:618 |
Rv3212 hyp |
hypothetical protein | 601 | 601 ctx | cooccurence:583 |
Rv2446c |
integral membrane protein | 577 | 578 ctx | cooccurence:575 |
Rv2138 lppL |
lipoprotein LppL | 577 | 578 ctx | cooccurence:575 |
Rv1109c hyp |
hypothetical protein | 546 | 546 ctx | cooccurence:545 |
Rv0481c hyp |
hypothetical protein | 537 | 537 ctx | cooccurence:537 |
Rv0773c ggtA |
bifunctional cephalosporin acylase/gamma-glutamyltranspeptidase | 519 | 519 ctx | neighborhood:516 |
Rv2091c |
membrane protein | 510 | 510 ctx | cooccurence:506 |
Rv0499 hyp |
hypothetical protein | 509 | 509 ctx | cooccurence:506 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: hypothetical protein
- MTBC0 PGAP product: TetR/AcrR family transcriptional regulator
- Pfam (hmmscan --cut_ga): TetR_N PF00440.30 (E=1e-07), TetR_C_31 PF17940.7 (E=2e-16)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215289.1)
- Domains: Pfam-A via hmmscan --cut_ga — TetR_N (PF00440.30), TetR_C_31 (PF17940.7)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG3226 - Curated reference: UniProt P71830 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
50 functional partner(s); context anchor
Rv3066 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_000824|Rv0775| MGVTAAVTPKGERRRYALVSAAAELLGEGGFEAVRHRAVARRAGLPLASTTYYFSSLDDLIARAVEHIGMIEVAQLRARVSALSRRRRGPETTAVVLVDLLVGEMSSPGLAEQLISRYERHIACTRLPDLRESMRRSLRQRAEAVAEAIERSGRSAQIELVCTLICAVDGSVVSALVEGRDPRAAALATVVDLIDVLAPVDQRPVPF