Rv0762c Family assigned · low auto-curated
H37Rv Rv0762c · MTBC0 mtbc0_000811 ·
181 aa · 860349–860894 (-) ·
RefSeq NP_215276.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | hypothetical protein |
|---|---|
| MTBC0 PGAP re-annotation | nuclear transport factor 2 family protein |
| Revised (this work) | Nuclear transport factor 2 family protein. |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
I6XW93
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Nuclear transport factor 2 family protein |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| Orthologous group | 28NTV |
|---|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | n/a |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 0 synonymous, 2 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
No Pfam-A domain above the gathering threshold (or not yet scanned).
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: cyp51 (lanosterol 14-alpha demethylase), high confidence from genomic context alone (score 992 excluding text-mining). This association is the citable seed of a function hypothesis for this hypothetical protein.
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0764c cyp51 |
lanosterol 14-alpha demethylase | 992 | 992 ctx | neighborhood:882 cooccurence:769 coexpression:744 |
Rv0765c |
oxidoreductase | 981 | 981 ctx | neighborhood:882 cooccurence:673 coexpression:553 |
Rv0763c |
ferredoxin | 977 | 977 ctx | neighborhood:882 cooccurence:750 |
Rv0767c |
HTH-type transcriptional regulator | 961 | 962 ctx | neighborhood:836 cooccurence:772 |
Rv0770 |
oxidoreductase | 955 | 956 ctx | neighborhood:677 cooccurence:531 coexpression:730 |
Rv0766c cyp123 |
cytochrome P450 Cyp123 | 952 | 952 ctx | neighborhood:882 coexpression:431 |
Rv0771 |
4-carboxymuconolactone decarboxylase | 935 | 936 ctx | neighborhood:677 cooccurence:679 coexpression:431 |
Rv0311 hyp |
hypothetical protein | 819 | 819 ctx | cooccurence:774 |
Rv0768 aldA |
aldehyde dehydrogenase AldA | 812 | 812 ctx | neighborhood:758 |
Rv0769 |
oxidoreductase | 811 | 811 ctx | neighborhood:731 |
Rv0761c adhB |
alcohol dehydrogenase B | 806 | 806 ctx | neighborhood:774 |
Rv0760c hyp |
hypothetical protein | 720 | 720 ctx | neighborhood:578 |
Rv1628c hyp |
hypothetical protein | 633 | 634 ctx | cooccurence:632 |
Rv0926c hyp |
hypothetical protein | 629 | 630 ctx | cooccurence:626 |
Rv0310c hyp |
hypothetical protein | 615 | 615 ctx | cooccurence:615 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: hypothetical protein
- MTBC0 PGAP product: nuclear transport factor 2 family protein
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215276.1)
- Domains: Pfam-A via hmmscan --cut_ga — none above threshold
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
28NTV - Curated reference: UniProt I6XW93 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
27 functional partner(s); context anchor
cyp51 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_000811|Rv0762c| MAGYPRDELEDVVHRWLQANRTAERRGDWTLLADFYTDDATYGWNVGPNEDVMCVGIDEIRDIALGQEMDGLQGWRYPYQRVVIDEKQGEVVGFWKQVATDANGAEQEVYGIGGSWFRYAGGGKWNWQRDFFDFGHVSALYLELIKAGKLSPGMQKRIERAVSGNKVPGYYPLGKTPVPLW