PPE49 Family assigned · medium auto-curated
H37Rv Rv3125c · MTBC0 - ·
391 aa · 3490476–3491651 (-) ·
RefSeq YP_177932.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | PPE family protein PPE49 |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | PPE family protein PPE49. Pfam: PPE (PF00823.26), PPE-SVP (PF12484.14). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
P9WHY5
SwissProt · reviewed
· Inferred from homology
|
|---|---|
| UniProt name | Uncharacterized PPE family protein PPE49 |
UniProt still lists this protein as Uncharacterized PPE family protein PPE49; the revised annotation above is ahead of the current UniProt record.
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
N Cell motility
|
|---|---|
| eggNOG description | PPE family |
| Orthologous group | COG5651 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains) pseudogene candidate
| pN/pS | 0.544 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 4 synonymous, 5 missense, 1 nonsense, 2 frameshift |
| Disruption | 3 distinct premature-stop/frameshift site(s); most common in 5.92% of strains (8590) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
PPE | PF00823.26 | 7.3e-63 | 5–167 | PPE family |
PPE-SVP | PF12484.14 | 9.9e-10 | 293–387 | PPE-SVP subfamily C-terminal region |
Functional interaction network (STRING v12, guilt-by-association)
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0894 |
transcriptional regulator | 797 | 797 | coexpression:797 |
Rv0602c tcrA |
two component DNA binding transcriptional regulator TcrA | 748 | 748 | coexpression:748 |
Rv0603 hyp |
hypothetical protein | 738 | 738 | coexpression:738 |
Rv3167c |
TetR family transcriptional regulator | 733 | 733 | coexpression:733 |
Rv1776c |
transcriptional regulator | 730 | 730 | coexpression:730 |
Rv3291c lrpA |
transcriptional regulator LrpA | 729 | 729 | coexpression:729 |
Rv3124 moaR1 |
transcriptional regulator MoaR | 824 | 703 | coexpression:703 textmining:434 |
Rv1773c |
transcriptional regulator | 703 | 703 | coexpression:703 |
Rv3111 moaC1 |
cyclic pyranopterin monophosphate synthase accessory protein | 771 | 651 | coexpression:651 |
Rv3126c hyp |
hypothetical protein | 585 | 585 ctx | neighborhood:501 |
Rv1359 |
transcriptional regulator | 493 | 494 | coexpression:494 |
Rv1918c PPE35 |
PPE family protein PPE35 | 422 | 423 | coexpression:423 |
Rv3736 |
AraC/XylS family transcriptional regulator | 415 | 416 | coexpression:416 |
Rv1675c cmr |
HTH-type transcriptional regulator Cmr | 408 | 409 | coexpression:409 |
Rv2957 |
PGL/p-HBAD biosynthesis glycosyltransferase | 561 | 141 | textmining:511 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): PPE family protein PPE49
- Pfam (hmmscan --cut_ga): PPE PF00823.26 (E=7e-63), PPE-SVP PF12484.14 (E=1e-09)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq YP_177932.1)
- Domains: Pfam-A via hmmscan --cut_ga — PPE (PF00823.26), PPE-SVP (PF12484.14)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG5651 - Curated reference: UniProt P9WHY5 (SwissProt, reviewed; Inferred from homology)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023, doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 — 21 functional partner(s)
- Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv3125c|PPE49 MVLGFSWLPPEINSARMFAGAGSGPLFAAASAWEGLAADLWASASSFESVLAALTTGPWTGPASMSMAAAASPYVGWLSTVASQAQLAAIQARAAATAFEAALAATVHPTAVTANRVSLASLIAANVLGQNTPAIAATEFDYLEMWAQDVAAMVGYHAGAKSVAATLAPFSLPPVSLAGLAAQVGTQVAGMATTASAAVTPVVEGAMASVPTVMSGMQSLVSQLPLQHASMLFLPVRILTSPITTLASMARESATRLGPPAGGLAAANTPNPSGAAIPAFKPLGGRELGAGMSAGLGQAQLVGSMSVPPTWQGSIPISMASSAMSGLGVPPNPVALTQAAGAAGGGMPMMLMPMSISGAGAGMPGGLMDRDGAGWHVTQARLTVIPRTGVG