moeB1 Resolved · high auto-curated
H37Rv Rv3206c · MTBC0 - ·
392 aa · 3582532–3583710 (-) ·
RefSeq YP_177942.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | adenylyltransferase/sulfurtransferase MoeZ |
|---|---|
| MTBC0 PGAP re-annotation | — |
| Revised (this work) | Adenylyltransferase/sulfurtransferase MoeZ. Pfam: ThiF (PF00899.28), Rhodanese (PF00581.26). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Annotated on the H37Rv protein: this gene has no 1:1 ancestral MTBC0 anchor (PE/PPE, paralogue, IS element, or otherwise unanchored CDS).
Curated reference (UniProt)
| UniProt |
P9WMN7
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Probable adenylyltransferase/sulfurtransferase MoeZ [Includes: Sulfur carrier protein CysO adenylyltransferase |
| EC (curated) |
EC 2.7.7.-, EC 2.8.1.-
|
| Curated function | Catalyzes the conversion of the sulfur carrier protein CysO to CysO-thiocarboxylate. The reaction is thought to proceed in two steps: first, ATP-dependent activation of CysO as acyl-adenylate (CysO-COOAMP), followed by sulfur transfer to give CysO-thiocarboxylate (CysO-COSH) (Probable). The sulfur source is unknown. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
H Coenzyme transport and metabolismP Inorganic ion transport and metabolism
|
|---|---|
| Preferred name | moeB |
| eggNOG description | The proteins in this cluster have high sequence similarity to MoeB and are possibly involved in the synthesis of molybdopterin, but there has been no biochemical or physiological characterization. There is also no genetic linkage to other molybdopterin cofactor synthesis proteins. These proteins are |
| Orthologous group | COG0476 |
| EC number |
EC 2.7.7.80, EC 2.8.1.11
|
| KEGG orthology |
K21147
|
| KEGG pathways |
map04122
|
| Gene Ontology (75) |
GO:0000096, GO:0000097, GO:0003674, GO:0003824, GO:0005575, GO:0005618, GO:0005622, GO:0005623, GO:0005737, GO:0005829, GO:0006082, GO:0006520 +63 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.23 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 3 synonymous, 2 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
ThiF | PF00899.28 | 5.5e-77 | 23–259 | ThiF family |
Rhodanese | PF00581.26 | 8.9e-18 | 290–383 | Rhodanese-like domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: cysO (sulfur carrier protein CysO), high confidence from genomic context alone (score 978 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3323c moaX |
MoaD-MoaE fusion protein MoaX | 996 | 989 | coexpression:444 experimental:473 database:934 textmining:718 |
Rv1335 cysO |
sulfur carrier protein CysO | 997 | 978 ctx | cooccurence:598 experimental:415 database:900 textmining:885 |
Rv3025c iscS |
cysteine desulfurase | 981 | 976 | database:960 |
Rv0868c moaD2 |
cyclic pyranopterin monophosphate synthase | 984 | 955 | experimental:415 database:900 textmining:670 |
Rv3112 moaD1 |
molybdenum cofactor biosynthesis protein MoaD | 989 | 945 | experimental:415 database:900 textmining:827 |
Rv3116 moeB2 |
molybdenum cofactor biosynthesis protein MoeB | 921 | 910 | database:900 |
Rv0866 moaE2 |
molybdopterin synthase catalytic subunit 2 | 910 | 852 | coexpression:434 textmining:422 |
Rv0417 thiG |
thiazole synthase | 880 | 836 | coexpression:729 |
Rv1334 mec |
[CysO | 819 | 808 | database:500 |
Rv3205c hyp |
hypothetical protein | 785 | 784 ctx | neighborhood:739 |
Rv0416 thiS |
sulfur carrier protein ThiS | 774 | 749 | coexpression:490 experimental:463 |
Rv3119 moaE1 |
molybdopterin synthase catalytic subunit 1 | 866 | 747 | coexpression:440 textmining:494 |
Rv3221A rshA |
anti-sigma factor RshA | 704 | 705 | coexpression:703 |
Rv0434 hyp |
hypothetical protein | 658 | 645 | database:595 |
Rv3846 sodA |
superoxide dismutase | 677 | 639 | experimental:573 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Annotation from H37Rv (no MTBC0 1:1 anchor; H37Rv protein used): adenylyltransferase/sulfurtransferase MoeZ
- Pfam (hmmscan --cut_ga): ThiF PF00899.28 (E=5e-77), Rhodanese PF00581.26 (E=9e-18)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq YP_177942.1)
- Domains: Pfam-A via hmmscan --cut_ga — ThiF (PF00899.28), Rhodanese (PF00581.26)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0476 - Curated reference: UniProt P9WMN7 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
132 functional partner(s); context anchor
cysO - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>H37Rv|Rv3206c|moeB1 MSTSLPPLVEPASALSREEVARYSRHLIIPDLGVDGQKRLKNARVLVIGAGGLGAPTLLYLAAAGVGTIGIVDFDVVDESNLQRQVIHGVADVGRSKAQSARDSIVAINPLIRVRLHELRLAPSNAVDLFKQYDLILDGTDNFATRYLVNDAAVLAGKPYVWGSIYRFEGQASVFWEDAPDGLGVNYRDLYPEPPPPGMVPSCAEGGVLGIICASVASVMGTEAIKLITGIGETLLGRLLVYDALEMSYRTITIRKDPSTPKITELVDYEQFCGVVADDAAQAAKGSTITPRELRDWLDSGRKLALIDVRDPVEWDIVHIDGAQLIPKSLINSGEGLAKLPQDRTAVLYCKTGVRSAEALAAVKKAGFSDAVHLQGGIVAWAKQMQPDMVMY