moaR1 Family assigned · medium auto-curated
H37Rv Rv3124 · MTBC0 mtbc0_003321 ·
289 aa · 3510871–3511740 (+) ·
RefSeq NP_217640.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | transcriptional regulator MoaR |
|---|---|
| MTBC0 PGAP re-annotation | transcriptional regulator MoaR1 |
| Revised (this work) | Transcriptional regulator MoaR1. Pfam: Trans_reg_C (PF00486.35), BTAD (PF03704.24). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
O05797
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Transcriptional regulatory protein MoaR1 |
| Curated function | Acts as a positive transcriptional regulator of the molybdopterin biosynthesis moa1 locus, promoting the expression of the moaA1B1C1D1 genes. Binds directly to the moaA1 promoter. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
K Transcription
|
|---|---|
| eggNOG description | involved in transcriptional mechanism. thought to regulate the biosynthesis of the mycobacterial cell wall arabinan and resistance to ethambutol (Emb |
| Orthologous group | COG3629 |
| Gene Ontology (78) |
GO:0003674, GO:0003676, GO:0003677, GO:0005488, GO:0006139, GO:0006163, GO:0006355, GO:0006725, GO:0006732, GO:0006753, GO:0006793, GO:0006796 +66 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.989 · relaxed/neutral |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 2 synonymous, 6 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Trans_reg_C | PF00486.35 | 1.2e-13 | 22–93 | Transcriptional regulatory protein, C terminal |
BTAD | PF03704.24 | 1.3e-48 | 101–246 | Bacterial transcriptional activator domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv0894 (transcriptional regulator), high confidence from genomic context alone (score 989 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0894 |
transcriptional regulator | 996 | 989 ctx | fusion:899 cooccurence:489 coexpression:802 textmining:663 |
Rv3055 |
TetR family transcriptional regulator | 885 | 883 ctx | cooccurence:544 coexpression:754 |
Rv3830c |
TetR family transcriptional regulator | 860 | 857 | coexpression:836 |
Rv0339c iniR |
transcriptional regulator | 860 | 856 | coexpression:785 |
Rv3263 |
DNA methylase | 849 | 850 | coexpression:805 |
Rv3840 |
transcriptional regulator | 847 | 847 | coexpression:827 |
Rv1674c |
transcriptional regulator | 845 | 845 | coexpression:845 |
Rv3167c |
TetR family transcriptional regulator | 839 | 836 | coexpression:836 |
Rv3113 |
phosphatase | 836 | 836 | coexpression:802 |
Rv2488c |
LuxR family transcriptional regulator | 842 | 834 | coexpression:780 |
Rv1189 sigI |
ECF RNA polymerase sigma factor SigI | 833 | 833 | coexpression:833 |
Rv1675c cmr |
HTH-type transcriptional regulator Cmr | 826 | 826 | coexpression:826 |
Rv0691c mftR |
mycofactocin biosynthesis transcriptional regulator MftR | 827 | 824 | coexpression:824 |
Rv1453 |
transcriptional activator protein | 824 | 824 | coexpression:798 |
Rv1190 hyp |
hypothetical protein | 815 | 815 | coexpression:815 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: transcriptional regulator MoaR
- MTBC0 PGAP product: transcriptional regulator MoaR1
- Pfam (hmmscan --cut_ga): Trans_reg_C PF00486.35 (E=1e-13), BTAD PF03704.24 (E=1e-48)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_217640.1)
- Domains: Pfam-A via hmmscan --cut_ga — Trans_reg_C (PF00486.35), BTAD (PF03704.24)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG3629 - Curated reference: UniProt O05797 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
73 functional partner(s); context anchor
Rv0894 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_003321|Rv3124|moaR1 MQFNVLGPLELNLRGTKLPLGTPKQRAVLAMLLLSRNQVVAADALVQAIWEKSPPARARRTVHTYICNLRRTLSDAGVDSRNILVSEPPGYRLLIGDRQQCDLDRFVAAKESGLRASAKGYFSEAIRYLDSALQNWRGPVLGDLRSFMFVQMFSRALTEDELLVHTKLAEAAIACGRADVVIPKLERLVAMHPYRESLWKQLMLGYYVNEYQSAAIDAYHRLKSTLAEELGVEPAPTIRALYHKILRQLPMDDLVGRVTRGRVDLRGGNGAKVEELTESDKDLLPIGLA