zapE Resolved · high auto-curated
H37Rv Rv2670c · MTBC0 mtbc0_002844 ·
369 aa · 3007934–3009043 (-) ·
RefSeq NP_217186.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | hypothetical protein |
|---|---|
| MTBC0 PGAP re-annotation | cell division protein ZapE |
| Revised (this work) | Cell division protein ZapE. Pfam: AFG1_ATPase (PF03969.23). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
I6Y1G3
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Cell division protein ZapE |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| eggNOG description | AFG1-like ATPase |
| Orthologous group | COG1485 |
| KEGG orthology |
K06916
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 2.202 · diversifying/relaxed |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 1 synonymous, 6 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
AFG1_ATPase | PF03969.23 | 2.4e-112 | 33–368 | AFG1-like ATPase |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: ribD (bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase), high confidence from genomic context alone (score 802 excluding text-mining). This association is the citable seed of a function hypothesis for this hypothetical protein.
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2671 ribD |
bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase | 802 | 802 ctx | neighborhood:792 |
Rv1382 hyp |
hypothetical protein | 794 | 794 ctx | cooccurence:770 |
Rv3317 sdhD |
succinate dehydrogenase hydrophobic membrane anchor subunit | 792 | 793 ctx | cooccurence:774 |
Rv3316 sdhC |
succinate dehydrogenase cytochrome B-556 subunit | 819 | 792 ctx | cooccurence:774 |
Rv3319 sdhB |
succinate dehydrogenase iron-sulphur protein subunit | 774 | 775 ctx | cooccurence:759 |
Rv2772c |
transmembrane protein | 774 | 774 ctx | cooccurence:774 |
Rv3318 sdhA |
succinate dehydrogenase flavoprotein subunit | 749 | 750 ctx | cooccurence:733 |
Rv2673 aftC |
alpha-(1->3)-arabinofuranosyltransferase | 558 | 559 ctx | neighborhood:553 |
Rv2672 |
protease | 553 | 553 ctx | neighborhood:548 |
Rv0194 |
multidrug ABC transporter ATPase/permease | 503 | 504 ctx | cooccurence:487 |
Rv2052c hyp |
hypothetical protein | 432 | 432 ctx | cooccurence:432 |
Rv2674 msrB |
peptide methionine sulfoxide reductase MsrB | 431 | 431 ctx | neighborhood:427 |
Rv3585 radA |
DNA repair protein RadA | 422 | 423 | coexpression:423 |
Rv0803 purL |
phosphoribosylformylglycinamidine synthase 2 | 418 | 419 | coexpression:411 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: hypothetical protein
- MTBC0 PGAP product: cell division protein ZapE
- Pfam (hmmscan --cut_ga): AFG1_ATPase PF03969.23 (E=2e-112)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_217186.1)
- Domains: Pfam-A via hmmscan --cut_ga — AFG1_ATPase (PF03969.23)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1485 - Curated reference: UniProt I6Y1G3 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
14 functional partner(s); context anchor
ribD - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_002844|Rv2670c|zapE MTLIAARRYSATMHGSASEACGSVDHLVDRHPTVSPVRLIAQLRPPPTFAEVSFATYRPDPVEPTQAAAVVACQDFCRQAVERRAGRKKWFGKRDVLPGVGLYLDGGFGVGKTHLLASAYYQLPGTGPDAPTCPKAFATFGELTQLAGVFGFADCIDLLANYTALCIDEFELDDPGNTTLISRLLSALVERGVSVAATSNTLPEQLGEGRFAAQDFLREINTLASIFTTVRIEGPDYRHRDLPPAPAPLSDEEVAARAARVEGATLDDFDALCAHLATMHPSRYLTLIEGVTAVFLTGVHGIDDQNVALRLVALVDRLYDAGIPVVASGAKLDTIFSEEMLAGGYRKKYLRATSRLLALTAGVIQAREP