Rv2772c Still unknown · low auto-curated
H37Rv Rv2772c · MTBC0 mtbc0_002951 ·
157 aa · 3103534–3104007 (-) ·
RefSeq NP_217288.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | transmembrane protein |
|---|---|
| MTBC0 PGAP re-annotation | hypothetical protein |
| Revised (this work) | Conserved hypothetical protein; no recognised domain. Function unknown. |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
I6YA75
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Probable conserved transmembrane protein |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
U Intracellular trafficking, secretion and vesicular transport
|
|---|---|
| eggNOG description | COG0457 FOG TPR repeat |
| Orthologous group | COG5010 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.0 · strong purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 2 synonymous, 0 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
No Pfam-A domain above the gathering threshold (or not yet scanned).
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: dapB (4-hydroxy-tetrahydrodipicolinate reductase), high confidence from genomic context alone (score 878 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv2773c dapB |
4-hydroxy-tetrahydrodipicolinate reductase | 894 | 878 ctx | neighborhood:876 |
Rv2771c hyp |
hypothetical protein | 815 | 815 ctx | neighborhood:782 |
Rv3316 sdhC |
succinate dehydrogenase cytochrome B-556 subunit | 775 | 776 ctx | cooccurence:774 |
Rv3317 sdhD |
succinate dehydrogenase hydrophobic membrane anchor subunit | 774 | 775 ctx | cooccurence:774 |
Rv1382 hyp |
hypothetical protein | 774 | 774 ctx | cooccurence:773 |
Rv2670c zapE hyp |
hypothetical protein | 774 | 774 ctx | cooccurence:774 |
Rv2774c hyp |
hypothetical protein | 766 | 765 ctx | neighborhood:764 |
Rv3319 sdhB |
succinate dehydrogenase iron-sulphur protein subunit | 692 | 693 ctx | cooccurence:692 |
Rv3318 sdhA |
succinate dehydrogenase flavoprotein subunit | 590 | 590 ctx | cooccurence:589 |
Rv0883c sepH hyp |
hypothetical protein | 528 | 529 ctx | cooccurence:525 |
Rv2775 |
GCN5-like N-acetyltransferase | 513 | 514 ctx | neighborhood:512 |
Rv2693c |
integral membrane protein | 511 | 511 ctx | cooccurence:511 |
Rv1081c |
membrane protein | 505 | 505 ctx | cooccurence:504 |
Rv0194 |
multidrug ABC transporter ATPase/permease | 492 | 490 ctx | cooccurence:490 |
Rv2052c hyp |
hypothetical protein | 477 | 477 ctx | cooccurence:477 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: transmembrane protein
- MTBC0 PGAP product: hypothetical protein
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_217288.1)
- Domains: Pfam-A via hmmscan --cut_ga — none above threshold
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG5010 - Curated reference: UniProt I6YA75 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
18 functional partner(s); context anchor
dapB - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_002951|Rv2772c| MTRRTLYVQLIIAFMCVAMVAYLVMLGRVAVAMIGSGRAAAAGLGLALLILPVIGLWAMIATLRAGFAYQRLARLIAEDGLDIDASALPRRASGRIQRDAADALFAAVRTELEDDADDWRRWYRLARAYDYAGDRRRAREAMKTALQLEGRARPGAR