Rv1877 Family assigned · medium auto-curated
H37Rv Rv1877 · MTBC0 mtbc0_001991 ·
687 aa · 2143943–2146006 (+) ·
RefSeq NP_216393.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | MFS-type transporter |
|---|---|
| MTBC0 PGAP re-annotation | MDR family MFS transporter |
| Revised (this work) | MDR family MFS transporter. Pfam: MFS_1 (PF07690.22), TRI12 (PF06609.19), Sugar_tr (PF00083.31). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WG85
SwissProt · reviewed
· Inferred from homology
|
|---|---|
| UniProt name | Uncharacterized MFS-type transporter Rv1877 |
UniProt still lists this protein as Uncharacterized MFS-type transporter Rv1877; the revised annotation above is ahead of the current UniProt record.
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
E Amino acid transport and metabolismG Carbohydrate transport and metabolismP Inorganic ion transport and metabolism
|
|---|---|
| eggNOG description | Major facilitator superfamily |
| Orthologous group | COG0477 |
| Gene Ontology (20) |
GO:0003674, GO:0005215, GO:0005575, GO:0005623, GO:0005886, GO:0005887, GO:0006810, GO:0008150, GO:0016020, GO:0016021, GO:0022857, GO:0031224 +8 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 1.736 · diversifying/relaxed |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 4 synonymous, 16 missense, 2 nonsense, 2 frameshift |
| Disruption | 4 distinct premature-stop/frameshift site(s); most common in 9.30% of strains (13506) · convergent |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
MFS_1 | PF07690.22 | 2.1e-41 | 33–426 | Major Facilitator Superfamily |
TRI12 | PF06609.19 | 7.9e-17 | 37–448 | Fungal trichothecene efflux pump (TRI12) |
Sugar_tr | PF00083.31 | 4.1e-16 | 60–197 | Sugar (and other) transporter |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: glnA3 (glutamine synthetase GlnA), high confidence from genomic context alone (score 807 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1879 hyp |
hypothetical protein | 820 | 808 ctx | neighborhood:806 |
Rv1878 glnA3 |
glutamine synthetase GlnA | 885 | 807 ctx | neighborhood:806 textmining:432 |
Rv1876 bfrA |
bacterioferritin BfrA | 821 | 783 ctx | neighborhood:782 |
Rv1250 |
MFS-type drug transporter | 455 | 328 | |
Rv2678c hemE |
uroporphyrinogen decarboxylase | 704 | 157 | textmining:664 |
Rv0507 mmpL2 |
transmembrane transport protein MmpL2 | 481 | 115 | textmining:438 |
Rv0908 ctpE |
metal cation transporter ATPase E | 404 | 61 | |
Rv2836c dinF |
DNA-damage-inducible protein DinF | 485 | 54 | textmining:478 |
Rv2923c hyp |
hypothetical protein | 659 | 50 | textmining:656 |
Rv0421c hyp |
hypothetical protein | 657 | 44 | textmining:656 |
Rv0194 |
multidrug ABC transporter ATPase/permease | 638 | 44 | textmining:638 |
Rv2303c |
antibiotic-resistance protein | 533 | 44 | textmining:532 |
Rv2840c hyp |
hypothetical protein | 490 | 44 | textmining:489 |
Rv1907c hyp |
hypothetical protein | 535 | 42 | textmining:535 |
Rv1053c |
Hypothetical protein; Rv1053c, (MTV017.06c), len: 91 aa. Hypothetical unknown protein. This region is a possible MT-complex-specific genomic | 656 | 41 | textmining:656 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: MFS-type transporter
- MTBC0 PGAP product: MDR family MFS transporter
- Pfam (hmmscan --cut_ga): MFS_1 PF07690.22 (E=2e-41), TRI12 PF06609.19 (E=8e-17), Sugar_tr PF00083.31 (E=4e-16)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216393.1)
- Domains: Pfam-A via hmmscan --cut_ga — MFS_1 (PF07690.22), TRI12 (PF06609.19), Sugar_tr (PF00083.31)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0477 - Curated reference: UniProt P9WG85 (SwissProt, reviewed; Inferred from homology)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
16 functional partner(s); context anchor
glnA3 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001991|Rv1877| MAGPTAPTTAPTAIRAGGPLLSPVRRNIIFTALVFGVLVAATGQTIVVPALPTIVAELGSTVDQSWAVTSYLLGGTVVVVVAGKLGDLLGRNRVLLGSVVVFVVGSVLCGLSQTMTMLAISRALQGVGAGAISVTAYALAAEVVPLRDRGRYQGVLGAVFGVNTVTGPLLGGWLTDYLSWRWAFWINVPVSIAVLTVAATAVPALARPPKPVIDYLGILVIAVATTALIMATSWGGTTYAWGSATIVGLLIGAAVALGFFVWLEGRAAAAILPPRLFGSPVFAVCCVLSFVVGFAMLGALTFVPIYLGYVDGASATASGLRTLPMVIGLLIASTGTGVLVGRTGRYKIFPVAGMALMAVAFLLMSQMDEWTPPLLQSLYLVVLGAGIGLSMQVLVLIVQNTSSFEDLGVATSGVTFFRVVGASFGTATFGALFVNFLDRRLGSALTSGAVPVPAVPSPAVLHQLPQSMAAPIVRAYAESLTQVFLCAVSVTVVGFILALLLREVPLTDIHDDADDLGDGFGVPRAESPEDVLEIAVRRMLPNGVRLRDIATQPGCGLGVAELWALLRIYQYQRLFEAVRLTDIGRHLHVPYQVFEPVFDRLVQTGYAARDGDILTLTPSGHRQVDSLAVLIRQWLLDHLAVAPGLKRQPDHQFEAALQHVTDAVLVQRDWYEDLGDLSESRQLAATT