tesB2 Resolved · high auto-curated
H37Rv Rv2605c · MTBC0 mtbc0_002773 ·
281 aa · 2955850–2956695 (-) ·
RefSeq NP_217121.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | acyl-CoA thioesterase II |
|---|---|
| MTBC0 PGAP re-annotation | acyl-CoA thioesterase II |
| Revised (this work) | Acyl-CoA thioesterase II. Pfam: 4HBT_3 (PF13622.13), Acyl_CoA_thio (PF02551.22), 4HBT_3C (PF20789.4). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
I6X4S7
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Probable acyl-CoA thioesterase II TesB2 |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
I Lipid transport and metabolism
|
|---|---|
| Preferred name | tesB |
| eggNOG description | Acyl-CoA thioesterase |
| Orthologous group | COG1946 |
| KEGG orthology |
K10805
|
| KEGG pathways |
map01040
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains) pseudogene candidate
| pN/pS | 0.244 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 4 synonymous, 3 missense, 0 nonsense, 1 frameshift |
| Disruption | 1 distinct premature-stop/frameshift site(s); most common in 2.03% of strains (2949) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
4HBT_3 | PF13622.13 | 2.0e-16 | 31–107 | Acyl-CoA thioesterase N-terminal domain |
Acyl_CoA_thio | PF02551.22 | 1.2e-48 | 141–269 | Acyl-CoA thioesterase |
4HBT_3C | PF20789.4 | 7.2e-26 | 142–269 | Acyl-CoA thioesterase C-terminal domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: snoP (glutamine amidotransferase SnoP), high confidence from genomic context alone (score 884 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1618 tesB1 |
acyl-CoA thioesterase II | 925 | 925 | database:900 |
Rv2604c snoP |
glutamine amidotransferase SnoP | 984 | 884 ctx | neighborhood:882 textmining:870 |
Rv2606c snzP |
pyridoxine biosynthesis protein | 980 | 860 ctx | neighborhood:823 textmining:870 |
Rv2603c |
transcriptional regulator | 771 | 771 ctx | neighborhood:757 |
Rv0154c fadE2 |
acyl-CoA dehydrogenase FadE2 | 589 | 575 | |
Rv2613c |
AP-4-A phosphorylase | 572 | 572 ctx | neighborhood:544 |
Rv0632c echA3 |
enoyl-CoA hydratase EchA3 | 577 | 561 | coexpression:423 |
Rv2614c thrS |
threonine--tRNA ligase | 550 | 551 ctx | neighborhood:544 |
Rv3671c marP |
serine protease | 533 | 516 | database:495 |
Rv0125 pepA |
serine protease PepA | 532 | 515 | database:495 |
Rv1223 htrA |
serine protease HtrA | 526 | 509 | database:495 |
Rv0983 pepD |
serine protease PepD | 526 | 508 | database:495 |
Rv0672 fadE8 |
acyl-CoA dehydrogenase FadE8 | 522 | 504 | |
Rv1043c hyp |
hypothetical protein | 521 | 503 | database:495 |
Rv2607 pdxH |
pyridoxine/pyridoxamine 5'-phosphate oxidase | 499 | 499 ctx | neighborhood:496 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: acyl-CoA thioesterase II
- MTBC0 PGAP product: acyl-CoA thioesterase II
- Pfam (hmmscan --cut_ga): 4HBT_3 PF13622.13 (E=2e-16), Acyl_CoA_thio PF02551.22 (E=1e-48), 4HBT_3C PF20789.4 (E=7e-26)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_217121.1)
- Domains: Pfam-A via hmmscan --cut_ga — 4HBT_3 (PF13622.13), Acyl_CoA_thio (PF02551.22), 4HBT_3C (PF20789.4)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1946 - Curated reference: UniProt I6X4S7 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
56 functional partner(s); context anchor
snoP - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_002773|Rv2605c|tesB2 MSIEEILDLEQLEVNIYRGSVFSPESGFLQRTFGGHVAGQSLVSAVRTVDPRYMVHSLHGYFLRPGDAKERTVFLVERIRDGGSFCTRRVNAVQHGETIFSMAASFQTEQEGITHQDVMPAAPPPDGLPGLNSIKVFDDAGFRQFDEWDVCIVPRERLRLLPGKASQQQVWLRHRDPLPDDPVLHICALAYMSDLTLLGSAQVNHLDVRDQLQVASLDHAMWFMRPFRADEWLLYDQSSPSASGGRALTRGEIFTRSGEMVAAVMQEGLTRHRRGHRSVGQ