nuoG Family assigned · medium auto-curated
H37Rv Rv3151 · MTBC0 mtbc0_003349 ·
806 aa · 3539448–3541868 (+) ·
RefSeq NP_217667.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | NADH-quinone oxidoreductase subunit G |
|---|---|
| MTBC0 PGAP re-annotation | NADH-quinone oxidoreductase subunit G |
| Revised (this work) | NADH-quinone oxidoreductase subunit G. Pfam: Fer2_4 (PF13510.12), Fer2 (PF00111.33), NADH-G_4Fe-4S_3 (PF10588.15), Fer4_Nqo3 (PF22117.3), Molybdopterin (PF00384.28), Molydop_binding (PF01568.28). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WIV9
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | NADH-quinone oxidoreductase subunit G |
| EC (curated) |
EC 7.1.1.-
|
| Curated function | NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (By similarity)..; FUNCTION: Plays a critical role in M.tuberculosis ability to inhibit apoptosis of infected macrophages; thus helps the bacterium in its struggle to resist th. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
C Energy production and conversion
|
|---|---|
| Preferred name | nuoG |
| eggNOG description | NADH-quinone oxidoreductase |
| Orthologous group | COG1034 |
| EC number |
EC 1.6.5.3
|
| KEGG orthology |
K00336
|
| KEGG pathways |
map00190, map01100
|
| KEGG modules |
M00144
|
| Gene Ontology (48) |
GO:0003674, GO:0003824, GO:0003954, GO:0005488, GO:0005575, GO:0005618, GO:0005623, GO:0005886, GO:0008150, GO:0008152, GO:0009405, GO:0010941 +36 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.304 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 13 synonymous, 11 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Fer2_4 | PF13510.12 | 3.8e-20 | 15–90 | 2Fe-2S iron-sulfur cluster binding domain |
Fer2 | PF00111.33 | 7.1e-06 | 21–81 | 2Fe-2S iron-sulfur cluster binding domain |
NADH-G_4Fe-4S_3 | PF10588.15 | 1.8e-15 | 101–139 | NADH-ubiquinone oxidoreductase-G iron-sulfur binding region |
Fer4_Nqo3 | PF22117.3 | 3.3e-20 | 157–220 | NADH-quinone oxidoreductase subunit 3, ferredoxin-like domain |
Molybdopterin | PF00384.28 | 2.3e-20 | 291–639 | Molybdopterin oxidoreductase |
Molydop_binding | PF01568.28 | 4.8e-09 | 730–785 | Molydopterin dinucleotide binding domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: nuoD (NADH-quinone oxidoreductase subunit D), high confidence from genomic context alone (score 1000 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv3148 nuoD |
NADH-quinone oxidoreductase subunit D | 999 | 1000 ctx | neighborhood:881 cooccurence:574 coexpression:968 experimental:997 database:781 |
Rv3150 nuoF |
NADH-quinone oxidoreductase subunit F | 999 | 1000 ctx | neighborhood:881 cooccurence:759 coexpression:976 experimental:997 database:830 textmining:840 |
Rv3157 nuoM |
NADH-quinone oxidoreductase subunit M | 999 | 1000 ctx | neighborhood:760 coexpression:970 experimental:928 database:800 textmining:450 |
Rv3145 nuoA |
NADH-quinone oxidoreductase subunit A | 999 | 1000 ctx | neighborhood:879 coexpression:884 experimental:928 database:583 textmining:662 |
Rv3158 nuoN |
NADH-quinone oxidoreductase subunit N | 999 | 1000 ctx | neighborhood:760 coexpression:953 experimental:928 database:566 textmining:804 |
Rv3147 nuoC |
NADH-quinone oxidoreductase subunit C | 999 | 1000 ctx | neighborhood:881 cooccurence:741 coexpression:966 experimental:997 database:781 |
Rv3154 nuoJ |
NADH-quinone oxidoreductase subunit J | 999 | 1000 ctx | neighborhood:764 cooccurence:745 coexpression:962 experimental:911 |
Rv3152 nuoH |
NADH-quinone oxidoreductase subunit H | 999 | 1000 ctx | neighborhood:764 coexpression:965 experimental:928 database:583 |
Rv3153 nuoI |
NADH-quinone oxidoreductase subunit I | 999 | 1000 ctx | neighborhood:764 cooccurence:744 coexpression:974 experimental:997 database:895 |
Rv3146 nuoB |
NADH-quinone oxidoreductase subunit B | 999 | 1000 ctx | neighborhood:881 coexpression:958 experimental:997 database:895 textmining:448 |
Rv3149 nuoE |
NADH-quinone oxidoreductase subunit E | 999 | 1000 ctx | neighborhood:881 cooccurence:770 coexpression:976 experimental:997 database:838 textmining:774 |
Rv3156 nuoL |
NADH-quinone oxidoreductase subunit L | 999 | 999 ctx | neighborhood:760 coexpression:964 experimental:911 textmining:591 |
Rv3143 |
response regulator | 999 | 999 ctx | cooccurence:740 experimental:997 |
Rv3155 nuoK |
NADH-quinone oxidoreductase subunit K | 999 | 998 ctx | neighborhood:764 coexpression:859 experimental:911 textmining:630 |
Rv0310c hyp |
hypothetical protein | 993 | 992 | coexpression:634 experimental:928 database:738 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: NADH-quinone oxidoreductase subunit G
- MTBC0 PGAP product: NADH-quinone oxidoreductase subunit G
- Pfam (hmmscan --cut_ga): Fer2_4 PF13510.12 (E=4e-20), Fer2 PF00111.33 (E=7e-06), NADH-G_4Fe-4S_3 PF10588.15 (E=2e-15), Fer4_Nqo3 PF22117.3 (E=3e-20), Molybdopterin PF00384.28 (E=2e-20), Molydop_binding PF01568.28 (E=5e-09)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_217667.1)
- Domains: Pfam-A via hmmscan --cut_ga — Fer2_4 (PF13510.12), Fer2 (PF00111.33), NADH-G_4Fe-4S_3 (PF10588.15), Fer4_Nqo3 (PF22117.3), Molybdopterin (PF00384.28), Molydop_binding (PF01568.28)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1034 - Curated reference: UniProt P9WIV9 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
191 functional partner(s); context anchor
nuoD - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_003349|Rv3151|nuoG MTQAADTDIRVGQPEMVTLTIDGVEISVPKGTLVIRAAELMGIQIPRFCDHPLLEPVGACRQCLVEVEGQRKPLASCTTVATDDMVVRTQLTSEIADKAQHGVMELLLINHPLDCPMCDKGGECPLQNQAMSNGRTDSRFTEAKRTFAKPINISAQVLLDRERCILCARCTRFSDQIAGDPFIDMQERGALQQVGIYADEPFESYFSGNTVQICPVGALTGTAYRFRARPFDLVSSPSVCEHCASGCAQRTDHRRGKVLRRLAGDDPEVNEEWNCDKGRWAFTYATQPDVITTPLIRDGGDPKGALVPTSWSHAMAVAAQGLAAARGRTGVLVGGRVTWEDAYAYAKFARITLGTNDIDFRARPHSAEEADFLAARIAGRHMAVSYADLESAPVVLLVGFEPEDESPIVFLRLRKAARRHRVPVYTIAPFATGGLHKMSGRLIKTVPGGEPAALDDLATGAVGDLLATPGAVIMVGERLATVPGGLSAAARLADTTGARLAWVPRRAGERGALEAGALPTLLPGGRPLADEVARAQVCAAWHIAELPAAAGRDADGILAAAADETLAALLVGGIEPADFADPDAVLAALDATGFVVSLELRHSAVTERADVVFPVAPTTQKAGAFVNWEGRYRTFEPALRGSTLQAGQSDHRVLDALADDMGVHLGVPTVEAAREELAALGIWDGKHAAGPHIAATGPTQPEAGEAILTGWRMLLDEGRLQDGEPYLAGTARTPVVRLSPDTAAEIGAADGEAVTVSTSRGSITLPCSVTDMPDRVVWLPLNSAGSTVHRQLRVTIGSIVKIGAGS