Rv1691 Family assigned · low
H37Rv Rv1691 · MTBC0 mtbc0_001799 ·
250 aa · 1927964–1928716 (+) ·
RefSeq NP_216207.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | hypothetical protein |
|---|---|
| MTBC0 PGAP re-annotation | hypothetical protein |
| Revised (this work) | TPR-repeat fold (PDB 2PL2); putative protein-protein-interaction scaffold. |
Curated reference (UniProt)
| UniProt |
O33193
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Tetratrico peptide repeat group 5 domain-containing protein |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| eggNOG description | Tetratricopeptide repeat |
| Orthologous group | COG0457 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.224 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 5 synonymous, 3 missense, 0 nonsense, 1 frameshift |
| Disruption | 1 distinct premature-stop/frameshift site(s); most common in 0.85% of strains (1237) · clonal |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
No Pfam-A domain above the gathering threshold (or not yet scanned).
Structural neighbours (Foldseek on the ESMFold model, exploratory)
ESMFold model confidence: mean pLDDT 96.1 (very high). A confident model makes the fold comparison meaningful.
Best matches against the PDB, ranked by Foldseek homology probability. A high probability / TM-score suggests a shared fold; unless flagged sig (E < 0.01) these are fold hypotheses, not assignments.
| Target | Prob | TM | E-value | Description |
|---|---|---|---|---|
2pl2-assembly1_B |
1.00 | 0.74 | 5.2e-03 sig | 2pl2-assembly1_B Crystal structure of TTC0263: a thermophilic TPR protein in Thermus thermophilus HB27 |
5g05-assembly1_K |
1.00 | 0.63 | 2.2e-03 sig | 5g05-assembly1_K Cryo-EM structure of combined apo phosphorylated APC |
5a31-assembly1_Y |
1.00 | 0.67 | 5.4e-03 sig | 5a31-assembly1_Y Structure of the human APC-Cdh1-Hsl1-UbcH10 complex. |
4ui9-assembly1_K |
1.00 | 0.61 | 5.7e-03 sig | 4ui9-assembly1_K Atomic structure of the human Anaphase-Promoting Complex |
2ho1-assembly1_A |
1.00 | 0.59 | 8.5e-03 sig | 2ho1-assembly1_A Functional Characterization of Pseudomonas Aeruginosa pilF |
8j8p-assembly1_C |
1.00 | 0.58 | 1.2e-02 | 8j8p-assembly1_C Structure of the four-component Paf1 complex from Saccharomyces eubayanus |
5m72-assembly1_A |
1.00 | 0.54 | 5.4e-03 sig | 5m72-assembly1_A Structure of the human SRP68-72 protein-binding domain complex |
8qoy-assembly1_A |
1.00 | 0.55 | 1.4e-02 | 8qoy-assembly1_A Capsular polysaccharide synthesis multienzyme of Actinobacillus Pleuropneumoniae serotype 3 |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv1692 (phosphatase), high confidence from genomic context alone (score 976 excluding text-mining). This association is the citable seed of a function hypothesis for this hypothetical protein.
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1692 |
phosphatase | 996 | 976 ctx | neighborhood:882 fusion:795 textmining:876 |
Rv2299c htpG |
chaperone protein HtpG | 933 | 926 | experimental:786 database:631 |
Rv2555c alaS |
alanine--tRNA ligase | 925 | 920 | experimental:775 database:644 |
Rv1695 ppnK |
inorganic polyphosphate/ATP-NAD kinase | 986 | 900 ctx | neighborhood:882 textmining:870 |
Rv1694 tlyA |
16S/23S rRNA (cytidine-2'-O)-methyltransferase TlyA | 946 | 886 ctx | neighborhood:882 textmining:548 |
Rv1693 hyp |
hypothetical protein | 984 | 883 ctx | neighborhood:882 textmining:870 |
Rv3457c rpoA |
DNA-directed RNA polymerase subunit alpha | 878 | 871 | experimental:668 database:607 |
Rv1696 recN |
DNA repair protein RecN | 981 | 869 ctx | neighborhood:867 textmining:866 |
Rv1390 rpoZ |
DNA-directed RNA polymerase subunit omega | 867 | 867 | experimental:515 database:594 |
Rv0696 mftF |
mycofactocin biosynthesis glycosyltransferase MftF | 823 | 805 | experimental:788 |
Rv0667 rpoB |
DNA-directed RNA polymerase subunit beta | 811 | 791 | experimental:476 database:592 |
Rv0492c |
GMC-type oxidoreductase | 799 | 791 | experimental:411 database:583 |
Rv2919c glnB |
nitrogen regulatory protein P-II | 805 | 787 | experimental:787 |
Rv0312 hyp |
hypothetical protein | 784 | 772 | experimental:455 database:567 |
Rv0350 dnaK |
chaperone protein DnaK | 781 | 769 | experimental:455 database:567 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- MTBC0 PGAP product: hypothetical protein
- Foldseek best: 2pl2-assembly1_B Crystal structure of TTC0263: a thermophilic TPR protein in The (prob 1.00, E=5e-03, TM=0.74)
- (structure-only promotion reviewed by hand, 2026-06-01)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216207.1)
- Domains: Pfam-A via hmmscan --cut_ga — none above threshold
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0457 - Curated reference: UniProt O33193 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Model confidence: ESMFold per-residue pLDDT (mean 96.1, very high)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
133 functional partner(s); context anchor
Rv1692 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001799|Rv1691| MVDDRQGRRGGRRPRSAAADNRPAFRDGPAIPPGIHARQLAPEIRRELSTLDRATADAVACHLVAAGELIDDDPEAALRHARAARVRASRIAAVREAVGIAAYRCGDWAQALAELRAARRMGSKSPLLALIADCERGLGRPQRAIELARGSEAVELSGDAADELRIVAAGARADLGQLEQALTVLSTPQLDPGRTGSTAARLFYAYAEILLALGRGDEALQWFLRSAAADIDGVTDAEDRVDELGAREQK