mctB Resolved · high auto-curated
H37Rv Rv1698 · MTBC0 mtbc0_001806 ·
314 aa · 1934760–1935704 (+) ·
RefSeq NP_216214.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | copper transporter MctB |
|---|---|
| MTBC0 PGAP re-annotation | copper transporter MctB |
| Revised (this work) | Copper transporter MctB. Pfam: MctB (PF11382.14). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WJ83
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Copper transporter MctB |
| Curated function | Pore-forming protein, which is involved in efflux of copper across the outer membrane. Essential for copper resistance and maintenance of a low intracellular copper concentration. Required for virulence. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| Preferred name | mctB |
| eggNOG description | copper ion homeostasis |
| Orthologous group | 2D1T0 |
| Gene Ontology (30) |
GO:0003674, GO:0005215, GO:0005575, GO:0005623, GO:0006810, GO:0006811, GO:0008150, GO:0009279, GO:0015267, GO:0015288, GO:0016020, GO:0016021 +18 more
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 1.449 · diversifying/relaxed |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 1 synonymous, 4 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
MctB | PF11382.14 | 1.3e-95 | 1–311 | Copper transport outer membrane protein, MctB |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: xerD (tyrosine recombinase XerD), high confidence from genomic context alone (score 761 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1697 steA hyp |
hypothetical protein | 993 | 992 ctx | neighborhood:851 cooccurence:750 coexpression:810 |
Rv1701 xerD |
tyrosine recombinase XerD | 761 | 761 ctx | neighborhood:754 |
Rv1696 recN |
DNA repair protein RecN | 761 | 761 ctx | neighborhood:756 |
Rv1699 pyrG |
CTP synthase | 757 | 758 ctx | neighborhood:754 |
Rv1700 |
NUDIX hydrolase | 757 | 758 ctx | neighborhood:754 |
Rv1695 ppnK |
inorganic polyphosphate/ATP-NAD kinase | 752 | 752 ctx | neighborhood:748 |
Rv1694 tlyA |
16S/23S rRNA (cytidine-2'-O)-methyltransferase TlyA | 750 | 750 ctx | neighborhood:748 |
Rv3207c hyp |
hypothetical protein | 691 | 692 ctx | cooccurence:684 |
Rv2905 lppW |
lipoprotein LppW | 623 | 624 ctx | cooccurence:618 |
Rv2609c |
membrane protein | 595 | 596 ctx | cooccurence:574 |
Rv1693 hyp |
hypothetical protein | 576 | 577 ctx | neighborhood:574 |
Rv1691 hyp |
hypothetical protein | 576 | 577 ctx | neighborhood:574 |
Rv1692 |
phosphatase | 573 | 574 ctx | neighborhood:574 |
Rv3415c hyp |
hypothetical protein | 564 | 564 ctx | cooccurence:564 |
Rv1083 hyp |
hypothetical protein | 549 | 549 ctx | cooccurence:549 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: copper transporter MctB
- MTBC0 PGAP product: copper transporter MctB
- Pfam (hmmscan --cut_ga): MctB PF11382.14 (E=1e-95)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216214.1)
- Domains: Pfam-A via hmmscan --cut_ga — MctB (PF11382.14)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
2D1T0 - Curated reference: UniProt P9WJ83 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
52 functional partner(s); context anchor
xerD - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001806|Rv1698|mctB MISLRQHAVSLAAVFLALAMGVVLGSGFFSDTLLSSLRSEKRDLYTQIDRLTDQRDALREKLSAADNFDIQVGSRIVHDALVGKSVVIFRTPDAHDDDIAAVSKIVGQAGGAVTATVSLTQEFVEANSAEKLRSVVNSSILPAGSQLSTKLVDQGSQAGDLLGIALLSNADPAAPTVEQAQRDTVLAALRETGFITYQPRDRIGTANATVVVTGGALSTDAGNQGVSVARFAAALAPRGSGTLLAGRDGSANRPAAVAVTRADADMAAEISTVDDIDAEPGRITVILALHDLINGGHVGHYGTGHGAMSVTVSQ