steA Family assigned · medium auto-curated
H37Rv Rv1697 · MTBC0 mtbc0_001805 ·
393 aa · 1933557–1934738 (+) ·
RefSeq NP_216213.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | hypothetical protein |
|---|---|
| MTBC0 PGAP re-annotation | putative cytokinetic ring protein SteA |
| Revised (this work) | Putative cytokinetic ring protein SteA. Pfam: SteA-like_C (PF12555.14). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
O33198
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | SteA-like C-terminal domain-containing protein |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
S Function unknown
|
|---|---|
| eggNOG description | membrane-anchored protein |
| Orthologous group | COG4825 |
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.0 · strong purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 1 synonymous, 0 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
SteA-like_C | PF12555.14 | 3.2e-17 | 334–384 | SteA-like C-terminal domain |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: mctB (copper transporter MctB), high confidence from genomic context alone (score 992 excluding text-mining). This association is the citable seed of a function hypothesis for this hypothetical protein.
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1698 mctB |
copper transporter MctB | 993 | 992 ctx | neighborhood:851 cooccurence:750 coexpression:810 |
Rv1695 ppnK |
inorganic polyphosphate/ATP-NAD kinase | 769 | 769 ctx | neighborhood:766 |
Rv1694 tlyA |
16S/23S rRNA (cytidine-2'-O)-methyltransferase TlyA | 768 | 768 ctx | neighborhood:766 |
Rv1701 xerD |
tyrosine recombinase XerD | 766 | 767 ctx | neighborhood:731 |
Rv3267 lcp1 hyp |
hypothetical protein | 766 | 766 | coexpression:732 |
Rv1700 |
NUDIX hydrolase | 736 | 736 ctx | neighborhood:733 |
Rv1699 pyrG |
CTP synthase | 715 | 716 ctx | neighborhood:713 |
Rv1696 recN |
DNA repair protein RecN | 654 | 654 ctx | neighborhood:651 |
Rv1693 hyp |
hypothetical protein | 608 | 608 ctx | neighborhood:606 |
Rv1691 hyp |
hypothetical protein | 607 | 607 ctx | neighborhood:606 |
Rv1692 |
phosphatase | 606 | 606 ctx | neighborhood:606 |
Rv2610c pimA |
alpha-(1-2)-phosphatidylinositol mannosyltransferase | 454 | 454 ctx | cooccurence:448 |
Rv1690 lprJ |
lipoprotein LprJ | 441 | 440 ctx | neighborhood:435 |
Rv2191 hyp |
hypothetical protein | 428 | 428 ctx | cooccurence:425 |
Rv3717 hyp |
hypothetical protein | 413 | 413 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: hypothetical protein
- MTBC0 PGAP product: putative cytokinetic ring protein SteA
- Pfam (hmmscan --cut_ga): SteA-like_C PF12555.14 (E=3e-17)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216213.1)
- Domains: Pfam-A via hmmscan --cut_ga — SteA-like_C (PF12555.14)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG4825 - Curated reference: UniProt O33198 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
15 functional partner(s); context anchor
mctB - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001805|Rv1697|steA MRMSALLSRNTSRPGLIGIARVDRNIDRLLRRVCPGDIVVLDVLDLDRITADALVEAEIAAVVNASSSVSGRYPNLGPEVLVTNGVTLIDETGPEIFKKVKDGAKVRLYEGGVYAGDRRLIRGTERTDHDIADLMREAKSGLVAHLEAFAGNTIEFIRSESPLLIDGIGIPDVDVDLRRRHVVIVADEPSGPDDLKSLKPFIKEYQPVLVGVGTGADVLRKAGYRPQLIVGDPDQISTEVLKCGAQVVLPADADGHAPGLERIQDLGVGAMTFPAAGSATDLALLLADHHGAALLVTAGHAANIETFFDRTRVQSNPSTFLTRLRVGEKLVDAKAVATLYRNHISGGAIALLALTMLIAIIVALWVSRTDGVVLHWIIDYWNRFSLWVQHLVS