mftF Resolved · high auto-curated
H37Rv Rv0696 · MTBC0 mtbc0_000737 ·
470 aa · 799605–801017 (+) ·
RefSeq NP_215210.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | mycofactocin biosynthesis glycosyltransferase MftF |
|---|---|
| MTBC0 PGAP re-annotation | mycofactocin biosynthesis glycosyltransferase MftF |
| Revised (this work) | Mycofactocin biosynthesis glycosyltransferase MftF. Pfam: Glyco_tranf_2_3 (PF13641.13), Glycos_transf_2 (PF00535.33), Glyco_tranf_2_2 (PF10111.15), Glyco_trans_2_3 (PF13632.13). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
P9WMX1
SwissProt · reviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Pre-mycofactocin glycosyltransferase |
| EC (curated) |
EC 2.4.1.-
|
| Curated function | Involved in the biosynthesis of the enzyme cofactor mycofactocin (MFT). Acts as a glycosyltransferase that catalyzes the oligoglycosylation of pre-mycofactocin (PMFT), adding up to nine beta-1,4-linked glucose residues. |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
M Cell wall / membrane / envelope biogenesis
|
|---|---|
| eggNOG description | glycosyl transferase, family 2 |
| Orthologous group | COG1216 |
| Gene Ontology (8) |
GO:0005575, GO:0005622, GO:0005623, GO:0005737, GO:0005829, GO:0044424, GO:0044444, GO:0044464
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.469 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 4 synonymous, 5 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Glyco_tranf_2_3 | PF13641.13 | 4.9e-10 | 82–290 | Glycosyltransferase like family 2 |
Glycos_transf_2 | PF00535.33 | 8.8e-26 | 85–241 | Glycosyl transferase family 2 |
Glyco_tranf_2_2 | PF10111.15 | 7.0e-13 | 86–297 | Glycosyltransferase like family 2 |
Glyco_trans_2_3 | PF13632.13 | 1.4e-12 | 159–331 | Glycosyl transferase family group 2 |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: mftE (mycofactocin system creatinine amidohydrolase family protein MftE), high confidence from genomic context alone (score 991 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv0695 mftE |
mycofactocin system creatinine amidohydrolase family protein MftE | 999 | 991 ctx | neighborhood:813 cooccurence:695 coexpression:860 textmining:929 |
Rv0697 mftG |
dehydrogenase | 968 | 967 ctx | neighborhood:881 coexpression:731 |
Rv0693 mftC |
mycofactocin radical SAM maturase MftC | 993 | 932 ctx | neighborhood:702 cooccurence:770 textmining:907 |
Rv0692 mftB |
mycofactocin system protein MftB | 994 | 931 ctx | neighborhood:702 cooccurence:769 textmining:929 |
Rv0694 mftD |
mycofactocin system heme/flavin oxidoreductase MftD | 986 | 856 ctx | neighborhood:702 cooccurence:493 textmining:907 |
Rv0691c mftR |
mycofactocin biosynthesis transcriptional regulator MftR | 846 | 808 ctx | cooccurence:714 |
Rv3529c hyp |
hypothetical protein | 824 | 805 | experimental:788 |
Rv1691 hyp |
hypothetical protein | 823 | 805 | experimental:788 |
Rv2267c stf3 hyp |
hypothetical protein | 823 | 805 | experimental:788 |
Rv1096 |
glycosyl hydrolase | 815 | 759 | coexpression:739 |
Rv1639c hyp |
hypothetical protein | 760 | 752 | coexpression:746 |
Rv1354c hyp |
hypothetical protein | 887 | 720 ctx | neighborhood:544 coexpression:411 textmining:614 |
Rv1364c |
sigma factor regulatory protein | 787 | 719 ctx | neighborhood:544 coexpression:409 |
Rv0690c hyp |
hypothetical protein | 638 | 638 ctx | neighborhood:630 |
Rv1990A |
Rv1990A, len: 111 aa. Possible dehydrogenase (fragment), similar to N-terminal part of several dehydrogenases and hypothetical proteins, e.g | 649 | 634 ctx | cooccurence:590 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: mycofactocin biosynthesis glycosyltransferase MftF
- MTBC0 PGAP product: mycofactocin biosynthesis glycosyltransferase MftF
- Pfam (hmmscan --cut_ga): Glyco_tranf_2_3 PF13641.13 (E=5e-10), Glycos_transf_2 PF00535.33 (E=9e-26), Glyco_tranf_2_2 PF10111.15 (E=7e-13), Glyco_trans_2_3 PF13632.13 (E=1e-12)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_215210.1)
- Domains: Pfam-A via hmmscan --cut_ga — Glyco_tranf_2_3 (PF13641.13), Glycos_transf_2 (PF00535.33), Glyco_tranf_2_2 (PF10111.15), Glyco_trans_2_3 (PF13632.13)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG1216 - Curated reference: UniProt P9WMX1 (SwissProt, reviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
45 functional partner(s); context anchor
mftE - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_000737|Rv0696|mftF MTATRLPDGFAVQVDRRVRVLGDGSALLGGSPTRLLRLAPAARGLLCDGRLKVRDEVSAELARILLDATVAHPRPPSGPSHRDVTVVIPVRNNASGLRRLVTSLRGLRVIVVDDGSACPVESDDFVGAHCDIEVLHHPHSKGPAAARNTGLAACTTDFVAFLDSDVTPRRGWLESLLGHFCDPTVALVAPRIVSLVEGENPVARYEALHSSLDLGQREAPVLPHSTVSYVPSAAIVCRSSAIRDVGGFDETMHSGEDVDLCWRLIEAGARLRYEPIALVAHDHRTQLRDWIARKAFYGGSAAPLAVRHPDKTAPLVISGGALMAWILMSIGTGLGRLASLVIAVLTGRRIARAMRCAETSFLDVLAVATRGLWAAALQLASAICRHYWPLALLAAILSRRCRRVVLIAAVVDGVVDWLRRREGADDDAEPIGPLTYLVLKRVDDLAYGAGLWYGVVRERNIGALKPQIRT