Rv1683 Resolved · high auto-curated
H37Rv Rv1683 · MTBC0 mtbc0_001791 ·
999 aa · 1919609–1922608 (+) ·
RefSeq NP_216199.1
Annotation: from legacy to revised
| Legacy (H37Rv / Mycobrowser) | bifunctional long-chain acyl-CoA synthase/lipase |
|---|---|
| MTBC0 PGAP re-annotation | acyl-CoA synthetase |
| Revised (this work) | Acyl-CoA synthetase. Pfam: Abhydrolase_1 (PF00561.27), AMP-binding (PF00501.35). |
Auto-curated: this verdict and function were generated by rules from PGAP + Pfam + Foldseek and have not been hand-reviewed.
Curated reference (UniProt)
| UniProt |
O33185
TrEMBL · unreviewed
· Evidence at protein level
|
|---|---|
| UniProt name | Possible bifunctional enzyme long-chain acyl-CoA synthase and lipase |
Functional vocabulary (eggNOG-mapper, orthology transfer)
| COG category |
I Lipid transport and metabolismQ Secondary metabolites biosynthesis, transport and catabolism
|
|---|---|
| eggNOG description | synthetase |
| Orthologous group | COG0318 |
| KEGG orthology |
K03821, K03822
|
| KEGG pathways |
map00650
|
Orthology-based transfer (eggNOG 5.0.2, diamond). EC/KO/GO/CAZy are computed annotations, not manual curation; cross-check against the primary literature before treating a specific reaction as established.
Conservation & selection (intra-MTBC, 145 209 strains)
| pN/pS | 0.35 · purifying |
|---|---|
| Polymorphic sites (≥ 0.1% of strains) | 8 synonymous, 8 missense, 0 nonsense, 0 frameshift |
pN/pS from segregating SNPs (singletons removed) normalised by possible sites. Low pN/pS = purifying selection (a strong signal that a "hypothetical" is a real, constrained gene). A high pN/pS is ambiguous: relaxed constraint or positive selection (drug resistance, antigenic variation) inflate it; e.g. rpoB/katG/pncA score high here for resistance, not loss of function. A clonal disruption (one allele over a clade) suggests lineage pseudogenisation; a convergent one (many independent alleles) is typical of resistance loss-of-function.
Domains (Pfam, hmmscan --cut_ga)
| Pfam | Accession | i-Evalue | Residues | Description |
|---|---|---|---|---|
Abhydrolase_1 | PF00561.27 | 3.7e-07 | 100–342 | alpha/beta hydrolase fold |
AMP-binding | PF00501.35 | 4.4e-28 | 455–783 | AMP-binding enzyme |
Functional interaction network (STRING v12, guilt-by-association)
Closest characterised functional partner: Rv2876 (transmembrane protein), medium confidence from genomic context alone (score 639 excluding text-mining).
| Partner | Product | Score | No text-mining | Channels (≥400) |
|---|---|---|---|---|
Rv1684 hyp |
hypothetical protein | 918 | 918 ctx | neighborhood:881 |
Rv2933 ppsC |
phthiocerol synthesis polyketide synthase type I PpsC | 776 | 705 | |
Rv2940c mas |
multifunctional mycocerosic acid synthase | 776 | 705 | |
Rv1527c pks5 |
polyketide synthase | 775 | 704 | |
Rv2048c pks12 |
polyketide synthase | 775 | 704 | |
Rv3825c pks2 |
phthioceranic/hydroxyphthioceranic acid synthase | 775 | 704 | |
Rv0719 rplF |
50S ribosomal protein L6 | 696 | 696 | experimental:402 database:510 |
Rv1125 hyp |
hypothetical protein | 675 | 673 ctx | cooccurence:550 |
Rv0464c hyp |
hypothetical protein | 696 | 671 ctx | cooccurence:662 |
Rv3807c |
decaprenylphosphoryl-5-phosphoribose phosphatase | 672 | 659 | database:500 |
Rv3800c pks13 |
polyketide synthase | 680 | 640 | |
Rv2876 |
transmembrane protein | 639 | 639 ctx | cooccurence:636 |
Rv2946c pks1 |
polyketide synthase | 675 | 635 | |
Rv0756c hyp |
hypothetical protein | 621 | 621 ctx | cooccurence:621 |
Rv1181 pks4 |
polyketide beta-ketoacyl synthase | 637 | 614 |
STRING combines evidence channels (neighborhood, fusion, cooccurrence, coexpression, experimental, database, text-mining) into a 0–1000 score. The ctx badge marks edges carried by the genomic-context channels (conserved neighborhood, fusion, phylogenetic co-occurrence), which are independent of orthology and structure and the strongest signal for an unknown gene. The no text-mining column recomputes the score from data alone, so a link that does not depend on the literature is visible. Association is a function hypothesis, not proof: corroborate with the operon context and the primary literature before assigning a function.
Evidence
- Legacy H37Rv annotation: bifunctional long-chain acyl-CoA synthase/lipase
- MTBC0 PGAP product: acyl-CoA synthetase
- Pfam (hmmscan --cut_ga): Abhydrolase_1 PF00561.27 (E=4e-07), AMP-binding PF00501.35 (E=4e-28)
- (auto-curated by rules from PGAP + Pfam + Foldseek; not hand-reviewed)
Sources
- Ancestral sequence & coordinates: Harrison LB et al. (2024), An imputed ancestral reference genome for the MTBC, doi:10.1101/2023.09.07.556366
- Product annotation: NCBI PGAP on MTBC0; legacy from H37Rv NC_000962.3 (RefSeq NP_216199.1)
- Domains: Pfam-A via hmmscan --cut_ga — Abhydrolase_1 (PF00561.27), AMP-binding (PF00501.35)
- Sequence-level signal: ESM Atlas (EvolutionaryScale × BioHub) — exploratory
- Controlled vocabulary: eggNOG-mapper 2.1.12 (Cantalapiedra et al. 2021,
doi:10.1093/molbev/msab293), eggNOG 5.0 DB
(Huerta-Cepas et al. 2019) — OG
COG0318 - Curated reference: UniProt O33185 (TrEMBL, unreviewed; Evidence at protein level)
- Intra-MTBC selection: pN/pS and disruption from SPDI variants of 145 209 MTBC strains (this work, local collection vs H37Rv NC_000962.3)
- Interaction network: STRING v12.0 (Szklarczyk et al. 2023,
doi:10.1093/nar/gkac1000), taxon 83332, CC-BY 4.0 —
146 functional partner(s); context anchor
Rv2876 - Primary literature: none located yet; annotation rests on the domain/homology sources above.
Ancestral MTBC0 protein sequence
>mtbc0_001791|Rv1683| MVDLNFSMVTRPIERLVATAQNGLEVLRLGGLETGSVPSPSQIVESVPMYKLRRYFPPDNRPGQPPVGPPVLMVHPMMMSADMWDVTREDGAVGILHASGLDPWVIDFGSPDEVEGGMRRNLADHIVALSEAVDTVKDATGHDVHFVGYSQGGMFCYQAAAYRRSKDIASVVAFGSPVDTLAALPMGIPANMGAAVADFMADHVFNRLDIPSWMARMGFQMMDPLKTAKARVDFVRQLHDREALLPREQQRRFLESEGWIAWSGPAISELLKQFIAHNRMMTGGFAISGQMVTLTDITCPILAFVGEVDDIGQPASVRGIRRAAPNSEVYECLIRAGHFGLVVGSRAAQQSWPTVADWVRWISGDGTKPENIHLMADQPAEHTDSGVAFSSRVAHGIGEVSEAALALARGAADAVVAANRSVRTLAVETVRTLPRLARLGQLNDHTRISLGRIIDEQAHDAPKGEFLLFDGRVHTYEAVNRRINNVVRGLIAVGVRQGDRVGVLMETRPSALVAIAALSRLGAVAVVMRPDTDLSASVRLGRVTEILTDPTNLDAARQLPGQVLVLGGGESRDLDLPADALEQGQVIDMEKIDPDAVELPAWYRPNPGLARDLAFIAFSSADGDLVAKQITNYRWAVSAFGTASTAALGRRDTVYCLTPLHHESALLVSLGGAVVGGTRIALSRGLRPDRFVAEVRQYGVTVVSYTWAMLRDVVDDPAFVLHGNHPVRLFIGSGMPTGLWERVVEAFAPAHVVEFFATTDGQAVLANVAGAKIGSKGRPLPGAGRVELGAYDAEHDLILENDRGFVQVAGVNQVGVLLAQSRGPIDPTASVKRGVFAPADTWISTDYLFWRDDDGDYWLAGGRGSVVRTARGMVYTEPVTNALGLITGVDLAVTYGVLVRGRHVAVSAVTLLPGATITAADLTEAVASMPVGLGPDIVHVVPQLTLSGTYRPTVSALRANGIPKAGRQAWYFNSGGNEYRRLTPAVRTELTGQHRRGNA